{PDOC51891}
{PS51891; CENP_V_GFA}
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* CENP-V/GFA domain profile *
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Centrome protein (CENP)-V is a kinetochore protein that drives the progression
of mitosis  by  coordinating  chromatin  condensation,  positioning  of sister
chromatid centromeres  and targeting of the chromosome passager complex (CPC),
a machinery  that  regulates  the  attachment of spindle microtubules (MTs) to
kinetochores. CENP-V  homologues  are  found  in all vertebrates as well as in
plants and nematodes. At the C-terminal end, CENP-V possesses an evolutionally
conserved domain  that  comprises  an  array of seven cysteines and shows high
structural similarity  to glutathione-dependent formaldehyde-activating enzyme
(Gfa), an  enzyme  responsible for formaldehyde detoxification in prokaryotes.
CENP-V could  be an enzyme that scavenges the formaldehyde produced in histone
demethylation reactions.  The  three  central  cysteines and the flanking four
cysteines separately  coordinate  to  zinc,  forming  a catalytic center and a
structural fold for a tertiary structure, respectively [1,2,3].

The main  topological feature of the CENP-V/GFA domain is an extremely twisted
mixed eight-stranded  beta-sheet  (see <PDB:1X6M>. Within this beta-sheet, the
strands beta8,  beta9,  and beta4 form a sandwich with another triple stranded
mixed beta-sheet (beta5, beta2, beta1). The interconnection between the sheets
is mediated  by  four  3(10)  helices  and  two  alpha  helices.  The sandwich
arrangement of  the  beta-strands  is  further buttressed by a terahedral zinc
coordinated by  the  side  chains of four cysteines. It appears that this zinc
atom has  only a structural role. A second zinc ion stabilizes a large hairpin
loop that connects beta3 and beta4 via residues of three cysteines [3].

The profile we developed covers the entire CENP-V/GFA domain.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: April 2019 / First entry.

[ 1] Tadeu A.M.B., Ribeiro S., Johnston J., Goldberg I., Gerloff D.,
     Earnshaw W.C.
     "CENP-V is required for centromere organization, chromosome alignment
     and cytokinesis."
     EMBO. J. 27:2510-2522(2008).
     PubMed=18772885; DOI=10.1038/emboj.2008.175
[ 2] Honda Z., Suzuki T., Honda H.
     "Identification of CENP-V as a novel microtubule-associating molecule
     that activates Src family kinases through SH3 domain interaction."
     Genes. Cells. 14:1383-1394(2009).
     PubMed=19930468; DOI=10.1111/j.1365-2443.2009.01355.x
[ 3] Neculai A.M., Neculai D., Griesinger C., Vorholt J.A., Becker S.
     "A dynamic zinc redox switch."
     J. Biol. Chem. 280:2826-2830(2005).
     PubMed=15548539; DOI=10.1074/jbc.C400517200

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