{PDOC51917}
{PS51917; PRU}
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* Pru (pleckstrin-like receptor for the Ub) domain profile *
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In eukaryotes,  selective  protein  degradation  is performed primarily by the
ubiquitin-proteasome pathway,  in  which  substrate  proteins  are  marked  by
covalently attached   ubiquitin   chains   that  mediate  recognition  by  the
proteasome. Rpn13  (also  called  ADRM1)  is  a subunit of the proteasome that
serves as  a  receptor  for  both ubiquitin and Uch37, one of the proteasome's
three deubiquitinating  enzymes.  Rpn13  binds  ubiquitin  though  a conserved
amino-terminal region  termed  the  pleckstrin-like  receptor for the Ub (Pru)
domain (also  known as the ubiquitin-binding domain, or UBD), which binds K48-
linked diubiquitin. Its carboxy-terminal DEUBAD domain (see <PDOC51916>) binds
deubiquinating enzyme  Uch37/UCHL5  and  enhances  its  isopeptidase  activity
[1,2,3].

The Pru   domain   forms  two  contiguous,  antiparallel  beta-sheets  with  a
configuration similar  to  the  pleckstrin-homology structural domain: a four-
stranded twisted   antiparallel   beta-sheet   (beta   1,  4-6)  packs  almost
orthogonally against   a   second   triple  stranded  beta-sheet  (beta  7-8).
Juxtaposed to  the  three-stranded  sheet are two beta-strands (beta 2-3). The
Pru domain forms a hydrophobic core containing conserved hydrophobic residues,
which are  located  within beta-sheets. One end of the beta-sandwich is capped
by a long C-terminal amphipatic alpha-helix (see <PDB:2R2Y>) [1,2,3].

The profile we developed covers the entire Pru domain.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: March 2020 / First entry.

[ 1] Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y.,
     Hofmann K., Walters K.J., Finley D., Dikic I.
     "Proteasome subunit Rpn13 is a novel ubiquitin receptor."
     Nature 453:481-488(2008).
     PubMed=18497817; DOI=10.1038/nature06926
[ 2] Schreiner P., Chen X., Husnjak K., Randles L., Zhang N., Elsasser S.,
     Finley D., Dikic I., Walters K.J., Groll M.
     "Ubiquitin docking at the proteasome through a novel
     pleckstrin-homology domain interaction."
     Nature 453:548-552(2008).
     PubMed=18497827; DOI=10.1038/nature06924
[ 3] Chen X., Lee B.-H., Finley D., Walters K.J.
     "Structure of proteasome ubiquitin receptor hRpn13 and its activation
     by the scaffolding protein hRpn2."
     Mol. Cell. 38:404-415(2010).
     PubMed=20471946; DOI=10.1016/j.molcel.2010.04.019

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