{PDOC51988}
{PS51988; HERPESVIRUS_UL32}
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* Herpesviridae UL32 packaging protein family profile *
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Herpesviruses are  large,  double-stranded  DNA  viruses  with  complex virion
assembly and  maturation  strategies.  Herpesviral  DNA packaging into nascent
capsids requires  multiple  conserved  viral  proteins  that coordinate genome
encapsidation. Proteins   of   the   UL32   family,  required  for  viral  DNA
encapsidation, form  homopentameric  rings  with  a positively charged central
channel that  binds double-stranded DNA. The central channel of ORF68 is lined
with positively  charged  residues that are necessary for nucleic acid binding
and production of infectious virions [1,2].

Each monomer  contains three zinc fingers and several bundles of alpha-helices
connected by  loops (see <PDB:6XF9>). UL32 contains several motifs with highly
conserved cysteines  and histidines that form three zinc fingers. The residues
that compose  the  first  two  zinc  fingers  are  perfectly  conserved in all
identified homologs  of  UL32. The third zinc finger is generally conserved in
alpha-and gamma-herpesviruses,  but is missing in the beta-herpesviruses. This
third zinc  finger  is  also  atypical  in  that two coordinating residues are
adjacent to each other [2].

Some proteins belonging to the UL32 packaging protein family are listed below:

 - Herpes simplex virus type 1 (HSV-1) UL32 protein [3].
 - Human cytomegalovirus (HCMV) UL52 protein [5].
 - Kaposi's sarcoma-associated virus (KSHV) ORF68 protein [1,2].
 - Epstein-Barr virus (EBV) BFLF1 protein [2].
 - Pseudorabies virus (PrV) UL32 protein [4].

The profile we developed covers the entire Herpesviridae UL32 packaging
protein family.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: October 2021 / First entry.

[ 1] Gardner M.R., Glaunsinger B.A.
     "Kaposi's Sarcoma-Associated Herpesvirus ORF68 Is a DNA Binding
     Protein Required for Viral Genome Cleavage and Packaging."
     J. Virol. 92:0-0(2018).
     PubMed=29875246; DOI=10.1128/JVI.00840-18
[ 2] Didychuk A.L., Gates S.N., Gardner M.R., Strong L.M., Martin A.,
     Glaunsinger B.A.
     "A pentameric protein ring with novel architecture is required for
     herpesviral packaging."
     Elife 10:0-0(2021).
     PubMed=33554858; DOI=10.7554/eLife.62261
[ 3] Heming J.D., Conway J.F., Homa F.L.
     "Herpesvirus Capsid Assembly and DNA Packaging."
     Adv. Anat. Embryol. Cell. Biol. 223:119-142(2017).
     PubMed=28528442; DOI=10.1007/978-3-319-53168-7_6
[ 4] Fuchs W., Klupp B.G., Granzow H., Leege T., Mettenleiter T.C.
     "Characterization of pseudorabies virus (PrV) cleavage-encapsidation
     proteins and  functional complementation of PrV pUL32 by the
     homologous protein of herpes simplex virus type 1."
     J. Virol. 83:3930-3943(2009).
     PubMed=19193798; DOI=10.1128/JVI.02636-08
[ 5] Muller C., Alain S., Gourin C., Baumert T.F., Ligat G., Hantz S.
     "New Insights into Human Cytomegalovirus pUL52 Structure."
     Viruses 13:0-0(2021).
     PubMed=34452502; DOI=10.3390/v13081638

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