AC   PRU01364;
DC   Domain;
TR   PROSITE; PS52020; CRESS_DNA_REP; 1; level=0
XX
Names: CRESS-DNA virus replication initiator protein (Rep) endonuclease domain
Function: The Rep endonuclease domain is characterized by rolling circle
 replication (RCR) motifs I through III, which are important for RCR
 initiation and termination. Motif I, UUTU (U denotes hydrophobic residues),
 is thought to be involved in the recognition of the origin of replication.
 Motif II, HU(H/Q), is involved in the coordination of divalent metal ions,
 Mg(2+) or Mn(2+), which are essential for endonuclease activity at the origin
 of replication. Motif III (YxxK, where x is any amino acid) is involved in
 dsDNA cleavage and subsequent covalent attachment of the Rep through the
 catalytic tyrosine residue to the 5' end of the cleaved product.
XX
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC       Note=Divalent metal cations, possibly Mg(2+) or Mn(2+).
end case
CC   -!- SUBCELLULAR LOCATION: Host nucleus.
XX
GO   GO:0006260; P:DNA replication
GO   GO:0003677; F:DNA binding
GO   GO:0042025; C:host cell nucleus
case <FTGroup:1>
GO   GO:0046872; F:metal ion binding
end case
case <FTTag:act_site>
GO   GO:0016888; F:endodeoxyribonuclease activity, producing 5'-phosphomonoesters
GO   GO:0016779; F:nucleotidyltransferase activity
end case
XX
KW   DNA-binding
KW   Host nucleus
KW   DNA replication
KW   Nucleotide-binding
case <FTTag:act_site>
KW   Endonuclease
KW   Hydrolase
KW   Nuclease
KW   Covalent protein-DNA linkage
KW   Nucleotidyltransferase
KW   Transferase
end case
case <FTGroup:1>
KW   Metal-binding
end case
XX
FT   From: PS52020
FT   DOMAIN          from..to
FT                   /note="CRESS-DNA virus Rep endonuclease #"
FT   MOTIF           8..11
FT                   /note="RCR-1"
FT   Condition: [FWILMVACGTSYPH](2)-T-[FWILMVACGTSYPH]
FT   MOTIF           47..49
FT                   /note="RCR-2"
FT   Condition: H-[FWILMVACGTSYPH]-[HQ]
FT   MOTIF           87..90
FT                   /note="RCR-3"
FT   Condition: Y-x(2)-K
FT   ACT_SITE        87
FT                   /note="For DNA cleavage activity"
FT   Tag: act_site; Condition: Y
FT   BINDING         39
FT                   /ligand="a divalent metal cation #1"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Group: 1; Condition: E
FT   BINDING         47
FT                   /ligand="a divalent metal cation #1"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Group: 1; Condition: H
FT   BINDING         49
FT                   /ligand="a divalent metal cation #1"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Group: 1; Condition: H
FT   BINDING         91
FT                   /ligand="a divalent metal cation #1"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Group: 1; Condition: [DEN]
XX
Chop: Nter=0; Cter=0;
Size: 94-130;
Related: None;
Repeats: 1;
Topology: Undefined;
Example: D4N3P2;
Scope:
 Viruses; Cressdnaviricota
 Bacteria; Candidatus Phytoplasma asteris
Comments: None;
XX
# Revision 1.1 2023/03/17
//