AC   PRU01366;
DC   Domain;
TR   PROSITE; PS52022; PV_NS1_NUC; 1; level=0
XX
Names: Parvovirus (PV) NS1 nuclease (NS1-Nuc) domain profile
 Parvovirus (PV) NS1 origin of replication binding (OBD) domain
 Parvovirus (PV) NS1 DNA-specific binding domain
Function: The PV NS1-Nuc domain plays an important role in the "rolling
 hairpin" replication of the single-stranded parvoviral DNA genome,
 recognizing origin of replication sequences in double-stranded DNA, and
 cleaving (i.e., nicking) single-stranded DNA at a nearby site known as the
 terminal resolution site (trs). Since the DNA encountered by NS1 is double-
 stranded, it is assumed that binding of NS1 to its target sequences in dsDNA
 induces strand separation nearby, allowing for the endonuclease activity of
 NS1 to cleave at the trs site.
XX
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=CHEBI:60240;
end case
CC   -!- SUBCELLULAR LOCATION: Host nucleus.
XX
GO   GO:0006260; P:DNA replication
GO   GO:0003677; F:DNA binding
GO   GO:0042025; C:host cell nucleus
case <FTGroup:1>
GO   GO:0046872; F:metal ion binding
end case
case <FTTag:act_site>
GO   GO:0004519; F:endonuclease activity
end case
XX
KW   DNA-binding
KW   Host nucleus
KW   DNA replication
KW   Nucleotide-binding
case <FTTag:act_site>
KW   Endonuclease
KW   Hydrolase
KW   Nuclease
KW   Covalent protein-DNA linkage
end case
case <FTGroup:1>
KW   Metal-binding
end case
XX
FT   From: PS52022
FT   DOMAIN          from..to
FT                   /note="PV NS1-Nuc #"
FT   MOTIF           100..102
FT                   /note="RCR-2"
FT   Condition: H-[FWILMVACGTSYPH]-H
FT   MOTIF           175..179
FT                   /note="RCR-3"
FT   Condition: Y-x(3)-K
FT   ACT_SITE        175
FT                   /note="For nuclease activity"
FT   Tag: act_site; Condition: Y
FT   BINDING         93
FT                   /ligand="a divalent metal cation #1"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Group: 1; Condition: [ED]
FT   BINDING         100
FT                   /ligand="a divalent metal cation #1"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Group: 1; Condition: H
FT   BINDING         102
FT                   /ligand="a divalent metal cation #1"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Group: 1; Condition: H
XX
Chop: Nter=0; Cter=0;
Size: 69-253;
Related: None;
Repeats: 1;
Topology: Undefined;
Example: P07300;
Scope:
 Viruses
Comments: None;
XX
# Revision 1.1 2023/05/05
//