AC   PRU01449;
DC   Domain;
TR   PROSITE; PS52105; HV_L_ENDO; 1; level=0
XX
Names: Hantaviridae family large (L) protein endonuclease (endoN) domain
Function: The endonuclease and cap-binding functions are required for viral
 transcription initiation via cap-snatching to provide short, capped RNA
 primers derived from host cell mRNAs.
XX
DE   Includes:
DE     RecName: Full=cap-snatching endonuclease;
DE              EC=3.1.-.-;
XX
case <FTGroup:1> and <FTGroup:2> and <FTTag:act_site>
CC   -!- COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC       Note=For endonuclease activity. Binds 2 Mn(2+) ions in the active site.
CC       The divalent metal ions are crucial for catalytic activity.
end case
CC   -!- DOMAIN: The N-terminus contains the endonuclease activity (endoN)
CC       (By similarity).
CC   -!- MISCELLANEOUS: Classified as His(+) endonuclease since it has a
CC       histidine upstream of the active site that coordinates the first
CC       cation.
CC   -!- SIMILARITY: Belongs to the Bunyavirales RNA polymerase family.
XX
case <FTGroup:1> and <FTGroup:2> and <FTTag:act_site>
GO   GO:0016787; F:hydrolase activity
GO   GO:0004519; F:endonuclease activity
end case
case <FTGroup:1> or <FTGroup:2>
GO   GO:0046872; F:metal ion binding
end case
GO   GO:0075526; P:cap snatching
GO   GO:0006351; P:DNA-templated transcription
XX
case <FTGroup:1> and <FTGroup:2> and <FTTag:act_site>
KW   Hydrolase
KW   Nuclease
KW   Endonuclease
end case
case <FTGroup:1> or <FTGroup:2>
KW   Manganese
KW   Metal-binding
end case
KW   Cap snatching
KW   Viral RNA replication
XX
FT   From: PS52105
FT   DOMAIN          from..to
FT                   /note="Cap-snatching endoN Hantaviridae-type #"
FT   ACT_SITE        124
FT                   /note="For endonuclease activity"
FT   Tag: act_site; Condition: K
FT   BINDING         36
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="#1"
FT   Group: 1; Condition: H
FT   BINDING         54
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="#2"
FT   Group: 2; Condition: E
FT   BINDING         97
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="#1"
FT   Group: 1; Condition: D
FT   BINDING         97
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="#2"
FT   Group: 2; Condition: D
FT   BINDING         110
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="#1"
FT   Group: 1; Condition: E
FT   BINDING         111
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="#1"
FT   Group: 1; Condition: V
XX
Chop: Nter=0; Cter=0;
Size: 217-217;
Related: None;
Repeats: 1;
Topology: Undefined;
Example: P23456;
Scope:
 Viruses; Orthohantavirus
Comments: None;
XX
# Version: 1
# Last updated date: 2026-05-21
# Created date: 2026-05-21
//