PROSITE documentation PDOC00164Phosphoribosylglycinamide synthetase signature
Phosphoribosylglycinamide synthetase (EC 6.3.4.13) (GARS) (phosphoribosylamine glycine ligase) [1] catalyzes the second step in the de novo biosynthesis of purine, the ATP-dependent addition of 5-phosphoribosylamine to glycine to form 5'phosphoribosylglycinamide.
In bacteria GARS is a monofunctional enzyme (encoded by the purD gene), in yeast it is part, with phosphoribosylformylglycinamidine cyclo-ligase (AIRS) of a bifunctional enzyme (encoded by the ADE5,7 gene), in higher eukaryotes it is part, with AIRS and with phosphoribosylglycinamide formyltransferase (GART) of a trifunctional enzyme (GARS-AIRS-GART).
The sequence of GARS is well conserved. As a signature pattern we selected a highly conserved octapeptide.
Last update:December 2001 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Aiba A. Mizobuchi K. |
| Title | Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli. | |
| Source | J. Biol. Chem. 264:21239-21246(1989). | |
| PubMed ID | 2687276 |
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