PROSITE documentation PDOC00164
Phosphoribosylglycinamide synthetase signature


Phosphoribosylglycinamide synthetase (EC (GARS) (phosphoribosylamine glycine ligase) [1] catalyzes the second step in the de novo biosynthesis of purine, the ATP-dependent addition of 5-phosphoribosylamine to glycine to form 5'phosphoribosylglycinamide.

In bacteria GARS is a monofunctional enzyme (encoded by the purD gene), in yeast it is part, with phosphoribosylformylglycinamidine cyclo-ligase (AIRS) of a bifunctional enzyme (encoded by the ADE5,7 gene), in higher eukaryotes it is part, with AIRS and with phosphoribosylglycinamide formyltransferase (GART) of a trifunctional enzyme (GARS-AIRS-GART).

The sequence of GARS is well conserved. As a signature pattern we selected a highly conserved octapeptide.

Last update:

December 2001 / Pattern and text revised.


Technical section

PROSITE method (with tools and information) covered by this documentation:

GARS, PS00184; Phosphoribosylglycinamide synthetase signature  (PATTERN)


1AuthorsAiba A. Mizobuchi K.
TitleNucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli.
SourceJ. Biol. Chem. 264:21239-21246(1989).
PubMed ID2687276

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