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PROSITE documentation PDOC00196 |
Clathrin [1,2] is the major coat-forming protein that encloses vesicles such as coated pits and forms cell surface patches involved in membrane traffic within eukaryotic cells. The clathrin coats (called triskelions) are composed of three heavy chains (180 Kd) and three light chains (23 to 27 Kd).
The clathrin light chains [3], which may help to properly orient the assembly and disassembly of the clathrin coats, bind non-covalently to the heavy chain, they also bind calcium and interact with the hsc70 uncoating ATPase.
We developed two signature patterns for clathrin light chains. The first pattern is a heptapeptide from the center of the conserved N-terminal region of eukaryotic light chains; the second pattern is derived from a positively charged region located in the C-terminal extremity of all known clathrin light chains.
Last update:December 2004 / Patterns and text revised.
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PROSITE methods (with tools and information) covered by this documentation:
1 | Authors | Keen J.H. |
Title | Clathrin and associated assembly and disassembly proteins. | |
Source | Annu. Rev. Biochem. 59:415-438(1990). | |
PubMed ID | 1973890 |
2 | Authors | Brodsky F.M. |
Title | Living with clathrin: its role in intracellular membrane traffic. | |
Source | Science 242:1396-1402(1988). | |
PubMed ID | 2904698 |
3 | Authors | Brodsky F.M. Hill B.L. Acton S.L. Nathke I. Wong D.H. Ponnambalam S. Parham P. |
Title | Clathrin light chains: arrays of protein motifs that regulate coated-vesicle dynamics. | |
Source | Trends Biochem. Sci. 16:208-213(1991). | |
PubMed ID | 1909824 |