PROSITE documentation PDOC00496Pyridine nucleotide-disulphide oxidoreductases class-II active site
Description
The pyridine nucleotide-disulphide oxidoreductases are FAD flavoproteins which contains a pair of redox-active cysteines involved in the transfer of reducing equivalents from the FAD cofactor to the substrate. On the basis of sequence and structural similarities [1] these enzymes can be classified into two categories. The second category groups together the following enzymes:
- Bacterial and fungal thioredoxin reductase (EC 1.8.1.9) (gene trxB) [2].
- Salmonella typhimurium alkyl hydroperoxide reductase protein F52a (gene aphF) [3], an enzyme that serves to protect the cell against damage to DNA by alkyl hydroperoxides.
- NADH dehydrogenase (EC 1.6.99.3) from Bacillus strain YN-1 (gene ndh) [4].
- A probable oxidoreductase which is encoded in the Clostridium pasteurianum rubredoxin operon [5].
- Yeast hypothetical protein YHR106w.
The sequence around the two cysteines involved in the redox-active disulfide bond is conserved and can be used as a signature pattern.
Last update:December 2004 / Pattern and text revised.
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Technical section
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References
| 1 | Authors | Kurlyan J. Krishna T.S.R. Wong L. Guenther B. Pahler A. Williams C.H. Jr. Model P. |
| Source | Nature 352:172-174(1991). |
| 2 | Authors | Russel M. Model P. |
| Title | Sequence of thioredoxin reductase from Escherichia coli. Relationship to other flavoprotein disulfide oxidoreductases. | |
| Source | J. Biol. Chem. 263:9015-9019(1988). | |
| PubMed ID | 3288628 |
| 3 | Authors | Tartaglia L.A. Storz G. Brodsky M.H. Lai A. Ames B.N. |
| Title | Alkyl hydroperoxide reductase from Salmonella typhimurium. Sequence and homology to thioredoxin reductase and other flavoprotein disulfide oxidoreductases. | |
| Source | J. Biol. Chem. 265:10535-10540(1990). | |
| PubMed ID | 2191951 |
| 4 | Authors | Xu X.M. Koyama N. Cui M. Yamagishi A. Nosoh Y. Oshima T. |
| Title | Nucleotide sequence of the gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp. strain YN-1. | |
| Source | J. Biochem. 109:678-683(1991). | |
| PubMed ID | 1917890 |
| 5 | Authors | Mathieu I. Meyer J. Moulis J.M. |
| Title | Cloning, sequencing and expression in Escherichia coli of the rubredoxin gene from Clostridium pasteurianum. | |
| Source | Biochem. J. 285:255-262(1992). | |
| PubMed ID | 1637309 |
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