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Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00496Pyridine nucleotide-disulphide oxidoreductases class-II active site
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00496
Description
The pyridine nucleotide-disulphide oxidoreductases are FAD flavoproteins which contains a pair of redox-active cysteines involved in the transfer of reducing equivalents from the FAD cofactor to the substrate. On the basis of sequence and structural similarities [1] these enzymes can be classified into two categories. The second category groups together the following enzymes:
- Bacterial and fungal thioredoxin reductase (EC 1.8.1.9) (gene trxB) [2].
- Salmonella typhimurium alkyl hydroperoxide reductase protein F52a (gene aphF) [3], an enzyme that serves to protect the cell against damage to DNA by alkyl hydroperoxides.
- NADH dehydrogenase (EC 1.6.99.3) from Bacillus strain YN-1 (gene ndh) [4].
- A probable oxidoreductase which is encoded in the Clostridium pasteurianum rubredoxin operon [5].
- Yeast hypothetical protein YHR106w.
The sequence around the two cysteines involved in the redox-active disulfide bond is conserved and can be used as a signature pattern.
Last update:December 2004 / Pattern and text revised.
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Technical section
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References
| 1 | Authors | Kurlyan J. Krishna T.S.R. Wong L. Guenther B. Pahler A. Williams C.H. Jr. Model P. |
| Source | Nature 352:172-174(1991). |
| 2 | Authors | Russel M. Model P. |
| Title | Sequence of thioredoxin reductase from Escherichia coli. Relationship to other flavoprotein disulfide oxidoreductases. | |
| Source | J. Biol. Chem. 263:9015-9019(1988). | |
| PubMed ID | 3288628 |
| 3 | Authors | Tartaglia L.A. Storz G. Brodsky M.H. Lai A. Ames B.N. |
| Title | Alkyl hydroperoxide reductase from Salmonella typhimurium. Sequence and homology to thioredoxin reductase and other flavoprotein disulfide oxidoreductases. | |
| Source | J. Biol. Chem. 265:10535-10540(1990). | |
| PubMed ID | 2191951 |
| 4 | Authors | Xu X.M. Koyama N. Cui M. Yamagishi A. Nosoh Y. Oshima T. |
| Title | Nucleotide sequence of the gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp. strain YN-1. | |
| Source | J. Biochem. 109:678-683(1991). | |
| PubMed ID | 1917890 |
| 5 | Authors | Mathieu I. Meyer J. Moulis J.M. |
| Title | Cloning, sequencing and expression in Escherichia coli of the rubredoxin gene from Clostridium pasteurianum. | |
| Source | Biochem. J. 285:255-262(1992). | |
| PubMed ID | 1637309 |
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