We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00532Glycosyl hydrolases family 32 active site
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00532
Description
It has been shown [1,2] that the following glycosyl hydrolases can be classified into a single family on the basis of sequence similarities:
- Inulinase (EC 3.2.1.7) (or inulase) from the fungi Kluyveromyces marxianus.
- β-fructofuranosidase (EC 3.2.1.26), commonly known as invertase in fungi and plants and as sucrase in bacteria (gene sacA or scrB).
- Raffinose invertase (EC 3.2.1.26) (gene rafD) from Escherichia coli plasmid pRSD2.
- Levanase (EC 3.2.1.65) (gene sacC) from Bacillus subtilis.
One of the conserved regions in these enzymes is located in the N-terminal section and contains an aspartic acid residue which has been shown [3], in yeast invertase to be important for the catalytic mechanism. We have used this region as a signature pattern.
Expert(s) to contact by email: Last update:April 2006 / Pattern revised.
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Technical section
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References
| 1 | Authors | Henrissat B. |
| Title | A classification of glycosyl hydrolases based on amino acid sequence similarities. | |
| Source | Biochem. J. 280:309-316(1991). | |
| PubMed ID | 1747104 |
| 2 | Authors | Gunasekaran P. Karunakaran T. Cami B. Mukundan A.G. Preziosi L. Baratti J. |
| Title | Cloning and sequencing of the sacA gene: characterization of a sucrase from Zymomonas mobilis. | |
| Source | J. Bacteriol. 172:6727-6735(1990). | |
| PubMed ID | 2254250 |
| 3 | Authors | Reddy V.A. Maley F. |
| Title | Identification of an active-site residue in yeast invertase by affinity labeling and site-directed mutagenesis. | |
| Source | J. Biol. Chem. 265:10817-10820(1990). | |
| PubMed ID | 2113524 |
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