PROSITE documentation PDOC00532Glycosyl hydrolases family 32 active site
Description
It has been shown [1,2] that the following glycosyl hydrolases can be classified into a single family on the basis of sequence similarities:
- Inulinase (EC 3.2.1.7) (or inulase) from the fungi Kluyveromyces marxianus.
- β-fructofuranosidase (EC 3.2.1.26), commonly known as invertase in fungi and plants and as sucrase in bacteria (gene sacA or scrB).
- Raffinose invertase (EC 3.2.1.26) (gene rafD) from Escherichia coli plasmid pRSD2.
- Levanase (EC 3.2.1.65) (gene sacC) from Bacillus subtilis.
One of the conserved regions in these enzymes is located in the N-terminal section and contains an aspartic acid residue which has been shown [3], in yeast invertase to be important for the catalytic mechanism. We have used this region as a signature pattern.
Expert(s) to contact by email: Last update:April 2006 / Pattern revised.
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References
1 | Authors | Henrissat B. |
Title | A classification of glycosyl hydrolases based on amino acid sequence similarities. | |
Source | Biochem. J. 280:309-316(1991). | |
PubMed ID | 1747104 |
2 | Authors | Gunasekaran P. Karunakaran T. Cami B. Mukundan A.G. Preziosi L. Baratti J. |
Title | Cloning and sequencing of the sacA gene: characterization of a sucrase from Zymomonas mobilis. | |
Source | J. Bacteriol. 172:6727-6735(1990). | |
PubMed ID | 2254250 |
3 | Authors | Reddy V.A. Maley F. |
Title | Identification of an active-site residue in yeast invertase by affinity labeling and site-directed mutagenesis. | |
Source | J. Biol. Chem. 265:10817-10820(1990). | |
PubMed ID | 2113524 |
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