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PROSITE documentation PDOC00640Glycosyl hydrolases family 8 signature
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The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [1,2]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family D [3] or as the glycosyl hydrolases family 8 [4,E1]. The enzymes which are currently known to belong to this family are listed below.
- Acetobacter xylinum endonuclease cmcAX.
- Bacillus strain KSM-330 acidic endonuclease K (Endo-K).
- Cellulomonas josui endoglucanase 2 (celB).
- Cellulomonas uda endoglucanase.
- Clostridium cellulolyticum endoglucanases C (celcCC).
- Clostridium thermocellum endoglucanases A (celA).
- Erwinia chrysanthemi minor endoglucanase y (celY).
- Bacillus circulans β-glucanase (EC 3.2.1.73).
- Escherichia coli hypothetical protein yhjM.
The most conserved region in these enzymes is a stretch of about 20 residues that contains two conserved aspartate. The first asparatate is thought [5] to act as the nucleophile in the catalytic mechanism. We have used this region as a signature pattern.
Expert(s) to contact by email: Last update:November 1997 / Text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Beguin P. |
| Title | Molecular biology of cellulose degradation. | |
| Source | Annu. Rev. Microbiol. 44:219-248(1990). | |
| PubMed ID | 2252383 | |
| DOI | 10.1146/annurev.mi.44.100190.001251 |
| 2 | Authors | Gilkes N.R. Henrissat B. Kilburn D.G. Miller R.C. Jr. Warren R.A.J. |
| Title | Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. | |
| Source | Microbiol. Rev. 55:303-315(1991). | |
| PubMed ID | 1886523 |
| 3 | Authors | Henrissat B. Claeyssens M. Tomme P. Lemesle L. Mornon J.-P. |
| Title | Cellulase families revealed by hydrophobic cluster analysis. | |
| Source | Gene 81:83-95(1989). | |
| PubMed ID | 2806912 |
| 4 | Authors | Henrissat B. |
| Title | A classification of glycosyl hydrolases based on amino acid sequence similarities. | |
| Source | Biochem. J. 280:309-316(1991). | |
| PubMed ID | 1747104 |
| 5 | Authors | Alzari P.M. Souchon H. Dominguez R. |
| Title | The crystal structure of endoglucanase CelA, a family 8 glycosyl hydrolase from Clostridium thermocellum. | |
| Source | Structure 4:265-275(1996). | |
| PubMed ID | 8805535 |
| E1 | Title | https://www.uniprot.org/docs/glycosid |
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