PROSITE documentation PDOC00690
Dihydroxy-acid and 6-phosphogluconate dehydratases signatures


Two dehydratases have been shown [1] to be evolutionary related:

  • Dihydroxy-acid dehydratase (EC (gene ilvD or ILV3) which catalyzes the fourth step in the biosynthesis of isoleucine and valine, the dehydratation of 2,3-dihydroxy-isovaleic acid into α-ketoisovaleric acid.
  • 6-phosphogluconate dehydratase (EC (gene edd) which catalyzes the first step in the Entner-Doudoroff pathway, the dehydratation of 6-phospho- D-gluconate into 6-phospho-2-dehydro-3-deoxy-D-gluconate.
  • Escherichia coli hypothetical protein yjhG.

Both enzymes are proteins of about 600 amino acid residues. We selected two highly conserved regions as signature patterns. The first pattern is located in the N-terminal part and contains a cysteine that could be involved in the binding of a 2Fe-2S iron-sulfur cluster [2]. The second pattern is located in the C-terminal half.

Last update:

December 2004 / Pattern and text revised.


Technical section

PROSITE methods (with tools and information) covered by this documentation:

ILVD_EDD_1, PS00886; Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1  (PATTERN)

ILVD_EDD_2, PS00887; Dihydroxy-acid and 6-phosphogluconate dehydratases signature 2  (PATTERN)


1AuthorsEgan S.E. Fliege R. Tong S. Shibata A. Wolf R.E. Jr. Conway T.
TitleMolecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon.
SourceJ. Bacteriol. 174:4638-4646(1992).
PubMed ID1624451

2AuthorsVelasco J.A. Cansado J. Pena M.C. Kawakami T. Laborda J. Notario V.
TitleCloning of the dihydroxyacid dehydratase-encoding gene (ILV3) from Saccharomyces cerevisiae.
SourceGene 137:179-185(1993).
PubMed ID8299945

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