Two dehydratases have been shown  to be evolutionary related:
Dihydroxy-acid dehydratase (EC 22.214.171.124) (gene ilvD or ILV3) which catalyzes
the fourth step in the biosynthesis of isoleucine and valine, the
dehydratation of 2,3-dihydroxy-isovaleic acid into α-ketoisovaleric
6-phosphogluconate dehydratase (EC 126.96.36.199) (gene edd) which catalyzes the
first step in the Entner-Doudoroff pathway, the dehydratation of 6-phospho-
D-gluconate into 6-phospho-2-dehydro-3-deoxy-D-gluconate.
Escherichia coli hypothetical protein yjhG.
Both enzymes are proteins of about 600 amino acid residues. We selected two
highly conserved regions as signature patterns. The first pattern is located
in the N-terminal part and contains a cysteine that could be involved in the
binding of a 2Fe-2S iron-sulfur cluster . The second pattern is located in
the C-terminal half.
December 2004 / Pattern and text revised.
PROSITE methods (with tools and information) covered by this documentation:
Egan S.E. Fliege R. Tong S. Shibata A. Wolf R.E. Jr. Conway T.
Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon.
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