PROSITE documentation PDOC00746Phosphomannose isomerase type I signatures
Phosphomannose isomerase (EC 5.3.1.8) (PMI) [1,2] is the enzyme that catalyzes the interconversion of mannose-6-phosphate and fructose-6-phosphate. In eukaryotes, it is involved in the synthesis of GDP-mannose which is a constituent of N- and O-linked glycans as well as GPI anchors. In prokaryotes, it is involved in a variety of pathways including capsular polysaccharide biosynthesis and D-mannose metabolism.
Three classes of PMI have been defined on the basis of sequence similarities [1]. The first class comprises all known eukaryotic PMI as well as the enzyme encoded by the manA gene in enterobacteria such as Escherichia coli. Class I PMI's are proteins of about 42 to 50 Kd which bind a zinc ion essential for their activity.
As signature patterns for class I PMI, we selected two conserved regions. The first one is located in the N-terminal section of these proteins, the second in the C-terminal half. Both patterns contain a residue involved [3] in the binding of the zinc ion.
Expert(s) to contact by email: Last update:November 1997 / Text revised.
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PROSITE methods (with tools and information) covered by this documentation:
| 1 | Authors | Proudfoot A.E.I. Turcatti G. Wells T.N. Payton M.A. Smith D.J. |
| Title | Purification, cDNA cloning and heterologous expression of human phosphomannose isomerase. | |
| Source | Eur. J. Biochem. 219:415-423(1994). | |
| PubMed ID | 8307007 |
| 2 | Authors | Coulin F. Magnenat E. Proudfoot A.E.I. Payton M.A. Scully P. Wells T.N.C. |
| Title | Identification of Cys-150 in the active site of phosphomannose isomerase from Candida albicans. | |
| Source | Biochemistry 32:14139-14144(1993). | |
| PubMed ID | 8260497 |
| 3 | Authors | Cleasby A. Wonacott A. Skarzynski T. Hubbard R.E. Davies G.J. Proudfoot A.E.I. Bernard A.R. Payton M.A. Wells T.N.C. |
| Title | The x-ray crystal structure of phosphomannose isomerase from Candida albicans at 1.7 angstrom resolution. | |
| Source | Nat. Struct. Biol. 3:470-479(1996). | |
| PubMed ID | 8612079 |
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