PROSITE documentation PDOC00772
CRISP family signature


A variety of extracellular proteins from eukaryotes have been found to be evolutionary related. They have been termed cysteine-rich secretory proteins (CRISP). Some relevant members of this family are:

  • Rodent sperm-coating glycoprotein (SCP), also known as acidic epididymal glycoprotein (AEG) . This protein is thought to be involved in sperm maturation [1]. It is a protein of about 220 residues and probably contains eight disulfide bonds.
  • Mammalian testis-specific protein Tpx-1 [2]. Tpx-1 is highly related to SCP's.
  • Mammalian glioma pathogenesis-related protein (GliPR).
  • Lizard helothermine, a toxin that blocks ryanodine receptors.
  • Venom allergen 5 (Ag5) from vespid wasps and venom allergen 3 (Ag3) from fire ants. These proteins are potent allergens and are the main cause of allergic reactions to stings from insects of the hymenoptera family [3]. Ag5/3 are proteins of about 200 residues and contain four disulfide bonds.
  • Plant pathogenesis proteins of the PR-1 family [4]. These proteins are synthesized during pathogen infection or other stress-related responses. They are proteins of about 130 to 140 residues and probably contain three disulfide bonds.
  • Proteins Sc7 and Sc14 from the basidomycete fungus Schizophyllum commune. These extracellular proteins are loosely associated with fruit body hyphal walls [5]. Sc7/14 are proteins of about 180 residues and probably contain two disulfide bonds.
  • Ancylostoma secreted protein from dog hookworm.
  • Yeast hypothetical proteins YJL078c, YJL079c and YKR013w.

The exact function of these proteins is not yet known. As signature patterns, we selected two conserved regions located in their C-terminal half. The second signature contains a cysteine which is known to be involved in a disulfide bond in Ag5.

Last update:

April 2006 / Pattern revised.


Technical section

PROSITE methods (with tools and information) covered by this documentation:

CRISP_1, PS01009; CRISP family signature 1  (PATTERN)

CRISP_2, PS01010; CRISP family signature 2  (PATTERN)


1AuthorsMizuki N. Kasahara M.
TitleMouse submandibular glands express an androgen-regulated transcript encoding an acidic epididymal glycoprotein-like molecule.
SourceMol. Cell. Endocrinol. 89:25-32(1992).
PubMed ID1301383

2AuthorsKasahara M. Gutknecht J. Brew K. Spurr N. Goodfellow P.N.
TitleCloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene.
SourceGenomics 5:527-534(1989).
PubMed ID2613236

3AuthorsLu G. Villalba M. Coscia M.R. Hoffman D.R. King T.P.
TitleSequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornets, wasps, and yellow jackets.
SourceJ. Immunol. 150:2823-2830(1993).
PubMed ID8454859

4AuthorsDixon D.C. Cutt J.R. Klessig D.F.
TitleDifferential targeting of the tobacco PR-1 pathogenesis-related proteins to the extracellular space and vacuoles of crystal idioblasts.
SourceEMBO J. 10:1317-1324(1991).
PubMed ID2026137

5AuthorsSchuren F.H.J. Asgeirsdottir S.A. Kothe E.M. Scheer J.M.J. Wessels J.G.
TitleThe Sc7/Sc14 gene family of Schizophyllum commune codes for extracellular proteins specifically expressed during fruit-body formation.
SourceJ. Gen. Microbiol. 139:2083-2090(1993).
PubMed ID8245835

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