PROSITE documentation PDOC00834Radical activating enzymes signature
In Escherichia coli and related bacteria, the pflA protein (or act) [1] is involved (EC 1.97.1.4) in the activation of pyruvate formate-lyase (gene pflB) under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine. A protein highly similar to pflA and termed pflC [2] is probably involved in the activation of a second pyruvate format lyase (gene pflD). The pflA/C proteins belong to a family that also includes phage T4 and Escherichia coli nrdG which are involved [3] in the generation of the free radical for the anaerobic ribonucleoside-triphosphate reductase (gene nrdD or sunY). This family also includes:
- Escherichia coli hypothetical protein yjjW.
- Haemophilus influenzae hypothetical protein HI0520.
- Methanococcus jannaschii hypothetical protein MJ0021.
All these proteins possess, in their N-terminal section, a highly conserved region which contains three clustered cysteines which are involved in the binding of a 4Fe4S iron-sulfur cluster [4]. We use this region as a signature pattern.
Last update:December 2004 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Roedel W. Plaga W. Frank R. Knappe J. |
| Source | Eur. J. Biochem. 177:153-158(1988). |
| 2 | Authors | Reizer J. Reizer A. Saier M.H. Jr. |
| Title | Novel phosphotransferase system genes revealed by bacterial genome analysis--a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system. | |
| Source | Microbiology 141:961-971(1995). | |
| PubMed ID | 7773398 |
| 3 | Authors | Sun X. Eliasson R. Pontis E. Andersson J. Buist G. Sjoeberg B.-M. Reichard P. |
| Title | Generation of the glycyl radical of the anaerobic Escherichia coli ribonucleotide reductase requires a specific activating enzyme. | |
| Source | J. Biol. Chem. 270:2443-2446(1995). | |
| PubMed ID | 7852304 |
| 4 | Authors | Tamarit J. Gerez C. Meier C. Mulliez E. Trautwein A. Fontecave M. |
| Title | The activating component of the anaerobic ribonucleotide reductase from Escherichia coli. An iron-sulfur center with only three cysteines. | |
| Source | J. Biol. Chem. 275:15669-15675(2000). | |
| PubMed ID | 10821845 |
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