PROSITE documentation PDOC00871Ribosomal RNA adenine dimethylases signature
Description
A number of enzymes responsible for the dimethylation of adenosines in ribosomal RNAs (EC 2.1.1.48) have been found [1,2] to be evolutionary related. These enzymes are:
- Bacterial 16S rRNA dimethylase (gene ksgA), which acts in the biogenesis of ribosomes by catalyzing the dimethylation of two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA. Inactivation of ksgA leads to resistance to the aminoglycoside antibiotic kasugamycin.
- Yeast 18S rRNA dimethylase (gene DIM1), which is functionally similar to ksgA and that dimethylates twin adenosines in the 3'-end of 18S rRNA.
- Bacterial 'erm' methylases. These enzymes confer resistance to macrolide- lincosamide-streptogramin B (MLS) antibiotics - such as erythromycin - by dimethylating the adenine residue at position 2058 of 23S rRNA thus resulting in a reduced affinity between ribosomes and the MLS antibiotics.
- Caenorhabditis elegans hypothetical protein EO2H1.1.
The best conserved region in these enzymes is located in the N-terminal section and corresponds to a region that is probably involved in S-adenosyl methionine (SAM) binding.
Last update:April 2006 / Pattern revised.
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References
| 1 | Authors | van Gemen B. van Knippenberg P.H. |
| Source | (In) Nucleic acid methylation, Clawson G.A., Willis D.B., Weissbach A., Jones P.A., Eds., pp.19-36, Alan R. Liss Inc, New-York, (1990). |
| 2 | Authors | Lafontaine D. Delcour J. Glasser A.L. Desgres J. Vandenhaute J. |
| Title | The DIM1 gene responsible for the conserved m6(2)Am6(2)A dimethylation in the 3'-terminal loop of 18 S rRNA is essential in yeast. | |
| Source | J. Mol. Biol. 241:492-497(1994). | |
| PubMed ID | 8064863 |
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