We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00871Ribosomal RNA adenine dimethylases signature
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00871
Description
A number of enzymes responsible for the dimethylation of adenosines in ribosomal RNAs (EC 2.1.1.48) have been found [1,2] to be evolutionary related. These enzymes are:
- Bacterial 16S rRNA dimethylase (gene ksgA), which acts in the biogenesis of ribosomes by catalyzing the dimethylation of two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA. Inactivation of ksgA leads to resistance to the aminoglycoside antibiotic kasugamycin.
- Yeast 18S rRNA dimethylase (gene DIM1), which is functionally similar to ksgA and that dimethylates twin adenosines in the 3'-end of 18S rRNA.
- Bacterial 'erm' methylases. These enzymes confer resistance to macrolide- lincosamide-streptogramin B (MLS) antibiotics - such as erythromycin - by dimethylating the adenine residue at position 2058 of 23S rRNA thus resulting in a reduced affinity between ribosomes and the MLS antibiotics.
- Caenorhabditis elegans hypothetical protein EO2H1.1.
The best conserved region in these enzymes is located in the N-terminal section and corresponds to a region that is probably involved in S-adenosyl methionine (SAM) binding.
Last update:April 2006 / Pattern revised.
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References
| 1 | Authors | van Gemen B. van Knippenberg P.H. |
| Source | (In) Nucleic acid methylation, Clawson G.A., Willis D.B., Weissbach A., Jones P.A., Eds., pp.19-36, Alan R. Liss Inc, New-York, (1990). |
| 2 | Authors | Lafontaine D. Delcour J. Glasser A.L. Desgres J. Vandenhaute J. |
| Title | The DIM1 gene responsible for the conserved m6(2)Am6(2)A dimethylation in the 3'-terminal loop of 18 S rRNA is essential in yeast. | |
| Source | J. Mol. Biol. 241:492-497(1994). | |
| PubMed ID | 8064863 |
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