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	PROSITE documentation PDOC01025

Crossover junction endodeoxyribonuclease ruvC signature

RuvC (EC 3.1.22.4) [1] is a bacterial nuclease that resolves Holliday junction intermediates in genetic recombination. It cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group.

It is a small protein of about 20 Kd. It requires and binds a magnesium ion. As a signature pattern we selected a region located in the C-terminal part. This region contains two aspartate residues implicated in the binding of the magnesium ion.

Last update:

April 2006 / Pattern revised.

PROSITE method (with tools and information) covered by this documentation:

RUVC, PS01321; Crossover junction endodeoxyribonuclease ruvC signature  (PATTERN)

• Consensus pattern:
  G-x-[GA]-x-[AG]-x-K-x-[EQA]-[IVM]-x(16,19)-D-x-[SAVT]-D-[AG]-x-[AGS]-[LIVMCA]-[ACS]
  The 2 D's are magnesium ligands
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 546
  • detected by PS01321: 495 (true positives)
  • undetected by PS01321: 51 (51 false negatives and 0 'partial')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS01321:
  NONE.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01321
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01321
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS01321
• View ligand binding statistics of PS01321
• Matching PDB structures: 1HJR [ALL]

1	Authors	West S.C.
	Title	Processing of recombination intermediates by the RuvABC proteins.
	Source	Annu. Rev. Genet. 31:213-244(1997).
	PubMed ID	9442895
	DOI	10.1146/annurev.genet.31.1.213

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