We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC01027Glycosyl hydrolases family 4 signature
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PURL: https://purl.expasy.org/prosite/documentation/PDOC01027
Description
It has been shown [1,2,E1] that the following glycosyl hydrolases can be, on the basis of sequence similarities, classified into a single family:
- Escherichia coli and Bacillus subtilis α-galactosidase (EC 3.2.1.22) (melibiase) (gene melA).
- Thermotoga maritima α-glucosidase (EC 3.2.1.20) (gene aglA).
- Probable 6-phospho-β-glucosidases (EC 3.2.1.86) (gene celF) from Escherichia coli and Bacillus subtilis.
- Maltose-6'-phosphate glucosidases (EC 3.2.1.122) (6-phospho-α-D- glucosidase) from Bacillus subtilis (gene glvG) and Fusobacterium mortiferum (gene malH).
- Bacillus subtilis protein lplD.
These enzymes require NAD and a divalent ion for their activity. They are proteins of about 50 Kd. As a signature pattern we selected a conserved region located in the central section. This region does not contain residues directly shown to be important for the catalytic activity.
Expert(s) to contact by email: Last update:April 2006 / Pattern revised.
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Technical section
PROSITE method (with tools and information) covered by this documentation:
References
| 1 | Authors | Henrissat B. |
| Title | A classification of glycosyl hydrolases based on amino acid sequence similarities. | |
| Source | Biochem. J. 280:309-316(1991). | |
| PubMed ID | 1747104 |
| 2 | Authors | Thompson J. Pikis A. Ruvinov S.B. Henrissat B. Yamamoto H. Sekiguchi J. |
| Title | The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily. | |
| Source | J. Biol. Chem. 273:27347-27356(1998). | |
| PubMed ID | 9765262 |
| E1 | Title | https://www.uniprot.org/docs/glycosid |
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