PROSITE documentation PDOC01027
Glycosyl hydrolases family 4 signature


It has been shown [1,2,E1] that the following glycosyl hydrolases can be, on the basis of sequence similarities, classified into a single family:

  • Escherichia coli and Bacillus subtilis α-galactosidase (EC (melibiase) (gene melA).
  • Thermotoga maritima α-glucosidase (EC (gene aglA).
  • Probable 6-phospho-β-glucosidases (EC (gene celF) from Escherichia coli and Bacillus subtilis.
  • Maltose-6'-phosphate glucosidases (EC (6-phospho-α-D- glucosidase) from Bacillus subtilis (gene glvG) and Fusobacterium mortiferum (gene malH).
  • Bacillus subtilis protein lplD.

These enzymes require NAD and a divalent ion for their activity. They are proteins of about 50 Kd. As a signature pattern we selected a conserved region located in the central section. This region does not contain residues directly shown to be important for the catalytic activity.

Expert(s) to contact by email:

Henrissat B.

Last update:

April 2006 / Pattern revised.


Technical section

PROSITE method (with tools and information) covered by this documentation:

GLYCOSYL_HYDROL_F4, PS01324; Glycosyl hydrolases family 4 signature  (PATTERN)


1AuthorsHenrissat B.
TitleA classification of glycosyl hydrolases based on amino acid sequence similarities.
SourceBiochem. J. 280:309-316(1991).
PubMed ID1747104

2AuthorsThompson J. Pikis A. Ruvinov S.B. Henrissat B. Yamamoto H. Sekiguchi J.
TitleThe gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily.
SourceJ. Biol. Chem. 273:27347-27356(1998).
PubMed ID9765262


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