|PROSITE documentation PDOC50007|
Phosphatidylinositol-specific phospholipase C (EC 126.96.36.199), an eukaryotic intracellular enzyme, plays an important role in signal transduction processes . It catalyzes the hydrolysis of 1-phosphatidyl-D-myo-inositol-4,5-diphosphate into the second messenger molecules diacylglycerol and inositol-1,4,5-triphosphate. This catalytic process is tightly regulated by reversible phosphorylation and binding of regulatory proteins [2,3,4].
In mammals, there are at least 6 different isoforms of PI-PLC, they differ in their domain structure, their regulation, and their tissue distribution. Lower eukaryotes also possess multiple isoforms of PI-PLC.
All eukaryotic PI-PLCs contain two regions of homology, sometimes referred to as 'X-box' and 'Y-box'. The order of these two regions is always the same (NH2-X-Y-COOH), but the spacing is variable. In most isoforms, the distance between these two regions is only 50-100 residues but in the γ isoforms one PH domain, two SH2 domains, and one SH3 domain are inserted between the two PLC-specific domains. The two conserved regions have been shown to be important for the catalytic activity. At the C-terminal of the Y-box, there is a C2 domain (see <PDOC00380>) possibly involved in Ca-dependent membrane attachment.
By profile analysis, we could show that sequences with significant similarity to the X-box domain occur also in prokaryotic and trypanosome PI-specific phospholipases C. Apart from this region, the prokaryotic enzymes show no similarity to their eukaryotic counterparts.
We developed two profiles, one covering the X-box, the other the Y-box.Last update:
July 2003 / Text revised.
PROSITE methods (with tools and information) covered by this documentation:
|1||Authors||Meldrum E. Parker P.J. Carozzi A.|
|Title||The PtdIns-PLC superfamily and signal transduction.|
|Source||Biochim. Biophys. Acta 1092:49-71(1991).|
|2||Authors||Rhee S.G. Choi K.D.|
|Title||Multiple forms of phospholipase C isozymes and their activation mechanisms.|
|Source||Adv. Second Messenger Phosphoprotein Res. 26:35-61(1992).|
|3||Authors||Rhee S.G. Choi K.D.|
|Title||Regulation of inositol phospholipid-specific phospholipase C isozymes.|
|Source||J. Biol. Chem. 267:12393-12396(1992).|
|4||Authors||Sternweis P.C. Smrcka A.V.|
|Title||Regulation of phospholipase C by G proteins.|
|Source||Trends Biochem. Sci. 17:502-506(1992).|