|PROSITE documentation PDOC50025|
The Laminin G or LNS domain (for Laminin-α, Neurexin and Sex hormone-binding globulin) is an around 180 amino acid long domain found in a large and diverse set of extracellular proteins [1,2]. It often occures in multiple copies probably serving as general protein interaction domains that bind the target proteins and other macromolecules, such as carbohydrates. In most proteins, the precise function of the laminin G domain is unknown. A large number of ligands in the G domain of laminin has been reported, including heparin, sulfatides, integrins, dystroglycan, nidogen, and fibulin [3,4,5]. In neurexin the G domain is known to bind neurexophilins, α-latrotoxin and neuroligins.
The crystal structure of Laminin G domain of neurexin 1-β has been solved . It is composed of 14 β strands and one α helix. The 14 strands form two antiparallel seven stranded β sheets in a jelly roll fold. The α helix is located between β strand 12 and 13. The structure of the laminin G domain is similar to the lectin domain. Alignment of various LNS with neurexin 1-β reveal that only the central part (between strand 4 and 13) is conserved.
Some proteins known to contain a laminin G domain are listed below:
The profile we developed covers the whole domain, including the highly divergent ends. We artificially placed the threshold at 12.1 to remove matches against thrombospondin-like N-terminal domain, a structurally homologue module. Thus we also remove few real matches which have a score between 8.5 and 12.1.Last update:
December 2001 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Vuolteenaho R. Chow L.T. Tryggvason K.|
|Title||Structure of the human laminin B1 chain gene.|
|Source||J. Biol. Chem. 265:15611-15616(1990).|
|2||Authors||Beckmann G. Hanke J. Bork P. Reich J.G.|
|Title||Merging extracellular domains: fold prediction for laminin G-like and amino-terminal thrombospondin-like modules based on homology to pentraxins.|
|Source||J. Mol. Biol. 275:725-730(1998).|
|3||Authors||Yurchenco P.D. Sung U. Ward M.D. Yamada Y. O'Rear J.J.|
|Title||Recombinant laminin G domain mediates myoblast adhesion and heparin binding.|
|Source||J. Biol. Chem. 268:8356-8365(1993).|
|4||Authors||Sung U. O'Rear J.J. Yurchenco P.D.|
|Title||Localization of heparin binding activity in recombinant laminin G domain.|
|Source||Eur. J. Biochem. 250:138-143(1997).|
|5||Authors||Talts J.F. Andac Z. Gohring W. Brancaccio A. Timpl R.|
|Title||Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins.|
|Source||EMBO J. 18:863-870(1999).|
|6||Authors||Rudenko G. Nguyen T. Chelliah Y. Sudhof T.C. Deisenhofer J.|
|Title||The structure of the ligand-binding domain of neurexin Ibeta: regulation of LNS domain function by alternative splicing.|