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PROSITE documentation PDOC50166 |
Nucleocytoplasmic transport is mediated by large supramolecular structures that span the nuclear envelope called nuclear pore complexes (NPC).
Classical import pathway involves proteins containing nuclear localization signal (NLS). This transport signal is recognized in the cytosol by importin α. Binding of the resulting complex to the cytoplasmic fibrils of the NPC is mediated by the cytosolic factor importin β. From there the substrate-α-β transport complex move to a central NPC region, where it pass through a gated channel [1,2]. This import as well as the export pathway are dependent on the GTPase cycle of Ran protein, a Ras-related GTPase.
Importin β proteins contain a conserved region in their N terminal part that is important for the binding of the Ran protein [3].
The eukaryotic proteins that contain such a domain are listed below:
The profile we developed covers the whole conserved region.
Last update:April 2002 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
1 | Authors | Mattaj I.W. Englmeier L. |
Title | Nucleocytoplasmic transport: the soluble phase. | |
Source | Annu. Rev. Biochem. 67:265-306(1998). | |
PubMed ID | 9759490 | |
DOI | 10.1146/annurev.biochem.67.1.265 |
2 | Authors | Melchior F. Gerace L. |
Title | Two-way trafficking with Ran. | |
Source | Trends Cell Biol. 8:175-179(1998). | |
PubMed ID | 9695834 |
3 | Authors | Vetter I.R. Arndt A. Kutay U. Gorlich D. Wittinghofer A. |
Title | Structural view of the Ran-Importin beta interaction at 2.3 A resolution. | |
Source | Cell 97:635-646(1999). | |
PubMed ID | 10367892 |