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PROSITE documentation PDOC50190SEC7 domain profile
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PURL: https://purl.expasy.org/prosite/documentation/PDOC50190
The SEC7 domain was named after the first protein found to contain such a region [1]. SEC7 family proteins are guanine nucleotide exchange factor (GEF) specific for the ADP-rybosylation factors (ARF), a Ras-like GTPases which is important for vesicular protein trafficking. Although SEC7 proteins are highly divergent in their overall sequence they share a common region of roughly 200 amino acids known as the SEC7 domain. Proteins containing a SEC7 domain are found in fungi, animals and plants.
SEC7 domains mediate interaction with ARF and catalyses GDP to GTP exchange [2,3,4]. Crystal structure of the human ARNO SEC7 domain has been solved [5,6] and consist of 10 α-helices. The helices are arranged in a right-handed superhelix reminiscent of that found in the armadillo-repeat (see <PDOC50176>). The C-terminal part of the domain mediates interaction with Arf-1 through hydrophobic and polar residues. The nucleotide exchange activity is dependent on a conserved glutamate residue located in the loop between helix f and g [5,6]. The corresponding residue in Arabidopsis Emb30 protein is mutated to a lysine in a deficient allele [7].
Some proteins known to contain a SEC7 domain are listed below.
- Yeast SEC7 protein. Associated with ER, essential for fusion of vesicles to the Golgi.
- Mammalian cytohesin 1.
- Mammalian cytohesin 2 (ARF nucleotide-binding site opener; ARNO).
- Mammalian cytohesin 3.
- Mammalian cytohesin 4.
- Mammalian brefeldin A-inhibited guanine nucleotide-exchange proteins 1 and 2 (ARFGEP1 and ARFGEP2).
- Mammalian golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1).
We developed a profile that spans the whole domain.
Last update:December 2001 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Achstetter T. Franzusoff A. Field C. Schekman R. |
| Title | SEC7 encodes an unusual, high molecular weight protein required for membrane traffic from the yeast Golgi apparatus. | |
| Source | J. Biol. Chem. 263:11711-11717(1988). | |
| PubMed ID | 3042778 |
| 2 | Authors | Klarlund J.K. Guilherme A. Holik J.J. Virbasius J.V. Chawla A. Czech M.P. |
| Title | Signaling by phosphoinositide-3,4,5-trisphosphate through proteins containing pleckstrin and Sec7 homology domains. | |
| Source | Science 275:1927-1930(1997). | |
| PubMed ID | 9072969 |
| 3 | Authors | Klarlund J.K. Rameh L.E. Cantley L.C. Buxton J.M. Holik J.J. Sakelis C. Patki V. Corvera S. Czech M.P. |
| Title | Regulation of GRP1-catalyzed ADP ribosylation factor guanine nucleotide exchange by phosphatidylinositol 3,4,5-trisphosphate. | |
| Source | J. Biol. Chem. 273:1859-1862(1998). | |
| PubMed ID | 9442017 |
| 4 | Authors | Abergel C. Chavrier P. Claverie J.-M. |
| Title | Triple association of CDC25-, Dbl- and Sec7-related domains in mammalian guanine-nucleotide-exchange factors. | |
| Source | Trends Biochem. Sci. 23:472-473(1998). | |
| PubMed ID | 9868368 |
| 5 | Authors | Cherfils J. Menetrey J. Mathieu M. Le Bras G. Robineau S. Beraud-Dufour S. Antonny B. Chardin P. |
| Title | Structure of the Sec7 domain of the Arf exchange factor ARNO. | |
| Source | Nature 392:101-105(1998). | |
| PubMed ID | 9510256 | |
| DOI | 10.1038/32210 |
| 6 | Authors | Mossessova E. Gulbis J.M. Goldberg J. |
| Title | Structure of the guanine nucleotide exchange factor Sec7 domain of human arno and analysis of the interaction with ARF GTPase. | |
| Source | Cell 92:415-423(1998). | |
| PubMed ID | 9476900 |
| 7 | Authors | Shevell D.E. Leu W.M. Gillmor C.S. Xia G. Feldmann K.A. Chua N.H. |
| Title | EMB30 is essential for normal cell division, cell expansion, and cell adhesion in Arabidopsis and encodes a protein that has similarity to Sec7. | |
| Source | Cell 77:1051-1062(1994). | |
| PubMed ID | 8020095 |
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