PROSITE documentation PDOC50600Ubiquitin-like protease family profile
Deubiquitinating enzymes (DUB) form a large family of cysteine protease that can deconjugate ubiquitin or ubiquitin-like proteins from ubiquitin-conjugated proteins. They can be classified in 3 families according to sequence homology [1,2]: ubiquitin carboxyl-terminal hydrolases (UCH) (see <PDOC00127>), ubiquitin-specific processing proteases (UBP) (see <PDOC00750>), and ubiquitin-like proteases (ULP) (EC 3.4.22.68) specific for deconjugating ubiquitin-like proteins. In contrast to the UBP pathway, which is very redundant (16 UBP enzymes in yeast), there is few ubiquitin-like protease (only one in yeast, ULP1).
ULP1 catalyses two critical functions in the SUMO/Smt3 pathway via its cysteine protease activity. ULP1 processes the Smt3 C-terminal sequence (-GGATY) to its mature form (-GG), and it deconjugates Smt3 from the lysine epsilon-amino group of the target protein [3].
Crystal structure of yeast ULP1 bound to Smt3 [4] revealed that the catalytic and interaction interface is situated in a shallow and narrow cleft where conserved residues recognize the Gly-Gly motif at the C-terminal extremity of Smt3 protein. Ulp1 adopts a novel architecture despite some structural similarity with other cysteine protease. The secondary structure is composed of seven α helices and seven β strands. The catalytic domain includes the central α helix, β-strands 4 to 6, and the catalytic triad (Cys-His-Asp) (see <PDB:1EUV>).
We developed a profile directed against the C-terminal part of ULP proteins that displays full proteolytic activity [4].
Note:These proteins belong to family C48 in the classification of peptidases [E1].
Last update:May 2008 / Text revised.
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PROSITE method (with tools and information) covered by this documentation:
1 | Authors | Chung C.H. Baek S.H. |
Title | Deubiquitinating enzymes: their diversity and emerging roles. | |
Source | Biochem. Biophys. Res. Commun. 266:633-640(1999). | |
PubMed ID | 10603300 | |
DOI | 10.1006/bbrc.1999.1880 |
2 | Authors | Hochstrasser M. |
Title | Ubiquitin-dependent protein degradation. | |
Source | Annu. Rev. Genet. 30:405-439(1996). | |
PubMed ID | 8982460 | |
DOI | 10.1146/annurev.genet.30.1.405 |
3 | Authors | Li S.J. Hochstrasser M. |
Title | A new protease required for cell-cycle progression in yeast. | |
Source | Nature 398:246-251(1999). | |
PubMed ID | 10094048 | |
DOI | 10.1038/18457 |
4 | Authors | Mossessova E. Lima C.D. |
Title | Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast. | |
Source | Mol. Cell 5:865-876(2000). | |
PubMed ID | 10882122 |
E1 | Title | https://www.uniprot.org/docs/peptidas |
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