PROSITE documentation PDOC50911

CHAP domain profile

The CHAP (cysteine, histidine-dependent amidohydrolases/peptidases) domain is a region between 110 and 140 amino acids that is found in proteins from bacteria, bacteriophages, archaea and eukaryotes of the Trypanosomidae family. Many of these proteins are uncharacterized, but it has been proposed that they may function mainly in peptidoglycan hydrolysis. The CHAP domain is found in a wide range of protein architectures; it is commonly associated with bacterial type SH3 domains and with several families of amidase domains. It has been suggested that CHAP domain containing proteins utilize a catalytic cysteine residue in a nucleophilic-attack mechanism [1,2].

The CHAP domain contains two invariant residues, a cysteine and a histidine. These residues form part of the putative active site of CHAP domain containing proteins. Secondary structure predictions show that the CHAP domain belongs to the α + β structural class, with the N-terminal half largely containing predicted α helices and the C-terminal half principally composed of predicted β strands [1,2].

Some proteins known to contain a CHAP domain are listed below:

• Bacterial and trypanosomal glutathionylspermidine amidases.
• A variety of bacterial autolysins.
• A Nocardia aerocolonigene putative esterase.
• Streptococcus pneumoniae choline-binding protein D.
• Methanosarcina mazei protein MM2478, a putative chloride channel.
• Several phage-encoded peptidoglycan hydrolases.

The profile we developed covers the entire CHAP domain.