Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC51025PWI domain profile
View entry in original PROSITE document format
View entry in raw text format (no links)
PURL: https://purl.expasy.org/prosite/documentation/PDOC51025
The PWI domain, named after a highly conserved PWI tri-peptide located within its N-terminal region, is a ~80 amino acid module, which is found either at the N-terminus or at the C-terminus of eukaryotic proteins involved in pre-mRNA processing [1]. It is found in association with other domains such as RRM (see <PDOC00030>) and RS. The PWI domain is a RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids and is likely to have multiple important functions in pre-mRNA processing [2].
The PWI domain is a soluble, globular and independently folded domain which consists of a four-helix bundle, with structured N- and C-terminal elements (see <PDB:1MP1>) [2].
Some proteins known to contain a PWI domain are listed below:
- Metazoan proteins homologous to SR-related nuclear matrix protein of 160 kD (SRm160).
- Mammalian splicing factor PRP3.
The profile we developed covers the entire PWI domain.
Last update:February 2007 / First entry.
-------------------------------------------------------------------------------
PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Blencowe B.J. Ouzounis C.A. |
| Title | The PWI motif: a new protein domain in splicing factors. | |
| Source | Trends Biochem. Sci. 24:179-180(1999). | |
| PubMed ID | 10322432 |
| 2 | Authors | Szymczyna B.R. Bowman J. McCracken S. Pineda-Lucena A. Lu Y. Cox B. Lambermon M. Graveley B.R. Arrowsmith C.H. Blencowe B.J. |
| Title | Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing. | |
| Source | Genes Dev. 17:461-475(2003). | |
| PubMed ID | 12600940 | |
| DOI | 10.1101/gad.1060403 |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.