PROSITE documentation PDOC51204

HSA domain profile

The helicase/SANT-associated (HSA) domain is a predicted DNA-binding domain of ~75 amino acids [1], which is found in the eukaryotic SRCAP/p400/DOM and SNF2/brahma families [2]. While each family has the core sequences that define the HSA domain, they each also have additional sequences that distinguish these families from one another. For example, the sequence HWDY(L/C)EEEM(Q/V) is found in the SRCAP/p400/DOM family, whereas the sequence HQE(Y/F)LNSILQ is found in the SNF2 /brahma family [2]. In addition to the SANT and helicase (see <PDOC51192>) domains, the HSA domain is also found in association with bromo (see <PDOC00550>) [1].

Some proteins known to contain a HSA domain are listed below [2]:

• Yeast transcription regulatory protein SWI2/SNF2.
• Drosophila brahma.
• Mammalian SNF2-α (BRM).
• Mammalian SNF2-β (BRG).
• Yeast SNF2-related CBP-activating protein (SRCAP), a coactivator for CREB- and nuclear hormone receptor-mediated transcriptional activation.
• Drosophila domino (DOM), the homolog of SCRAP. It is essential for organismal viability and has been implicated in several aspects of fly development, including hematopoeisis, wing development, and female fertility.
• Mammalian SRCAP.
• Mammalian SWI2/SNF2-like ATPase-containing p400/Domino.

The profile we developed covers the entire HSA domain.