|PROSITE documentation PDOC51210|
The PLA2c domain is the catalytic lipase domain in cytosolic phospholipase A2 (cPLA2) (EC 18.104.22.168) and lysophospholipase or phospholipase B (PLB) (EC 22.214.171.124) of vertebrates and fungi. It catalyzes the carboxylic ester hydrolysis of glycerophospholipids or lysophospholipids. The mammalian cPLA2 group IVA enzymes cleave intracellular phospholipid membranes to produce lipid mediators, which also play a role in inflammatory diseases such as asthma and arthritis. This enzyme contains a N-terminal calcium-binding C2 domain (see <PDOC00380>) that presents the catalytic domain to the membrane . Fungal secreted lysophospholipase/PLB can possess three different enzymatic activities, the hydrolase activity of phospholipase, lysophospholipase and a lysophospholipase transacylase activity.
The 3D structure of the PLA2c domain forms a central core of a 10-stranded mixed β sheet surrounded by 9 α helices (see <PDB:1CJY>). This central α/β core resembles the α/β hydrolase fold, but an additional 180-residue patch between strands 9 and 10 forms a catalytic domain 'cap' . Ser 228 and Asp 549 of human cPLA2 α form the potential catalytic dyad. Parts of the cap-region are poorly conserved and are distinct among isoforms [3,4].
Some proteins known to contain a PLA2c domain:
The profile we developed covers the entire PLA2c domain.Last update:
May 2006 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Nalefski E.A. Sultzman L.A. Martin D.M. Kriz R.W. Towler P.S. Knopf J.L. Clark J.D.|
|Title||Delineation of two functionally distinct domains of cytosolic phospholipase A2, a regulatory Ca(2+)-dependent lipid-binding domain and a Ca(2+)-independent catalytic domain.|
|Source||J. Biol. Chem. 269:18239-18249(1994)|
|2||Authors||Dessen A. Tang J. Schmidt H. Stahl M. Clark J.D. Seehra J. Somers W.S.|
|Title||Crystal structure of human cytosolic phospholipase A2 reveals a novel topology and catalytic mechanism.|
|3||Authors||Song C. Chang X.J. Bean K.M. Proia M.S. Knopf J.L. Kriz R.W.|
|Title||Molecular characterization of cytosolic phospholipase A2-beta.|
|Source||J. Biol. Chem. 274:17063-17067(1999).|
|4||Authors||Ohto T. Uozumi N. Hirabayashi T. Shimizu T.|
|Title||Identification of novel cytosolic phospholipase A(2)s, murine cPLA(2)delta, epsilon, and zeta, which form a gene cluster with cPLA(2)beta.|
|Source||J. Biol. Chem. 280:24576-24583(2005).|