PROSITE documentation PDOC51470FG-GAP repeat profile
Integrins are cell adhesion receptors that transmit bidirectional signals across the plasma membrane and link the extracellular environment to the actin cytoskeleton [1]. Integrins are formed from α and β subunits. The N-terminal region of α subunits contains seven 60 amino acids sequence repeats which fold into a β-propeller domain [2]. Integrins bind ligands at the interface between the α subunit β-propeller domain and β subunit I domain [3]. These domains associate over a large interface to form the integrin head. The seven FG-GAP repeat domain is also found in the enzyme phosphatidylinositol phospholipase D.
The crystal structure of the β-propeller FG-GAP domain of integrin α V shows that the domain consists of seven radially arranged "blades" each formed from a four-stranded antiparallel sheet (ABCD) (see <PDB:1JV2>) [4]. The inner strand (strand A) of each blade lines the channel at the center of the propeller, and strand D of the next repeat forming the outer edge of the blade. The β-propeller is circularized by juxtaposition of the C7 and D1 strands. Several Ca2+-binding sites are found in hairpin loops connecting β strands.
The profile we developed covers strand B,C,D and A of the FG-GAP repeat.
Last update:December 2009 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Springer T.A. |
| Title | Folding of the N-terminal, ligand-binding region of integrin alpha-subunits into a beta-propeller domain. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 94:65-72(1997). | |
| PubMed ID | 8990162 |
| 2 | Authors | Loftus J.C. Smith J.W. Ginsberg M.H. |
| Title | Integrin-mediated cell adhesion: the extracellular face. | |
| Source | J. Biol. Chem. 269:25235-25238(1994). | |
| PubMed ID | 7929213 |
| 3 | Authors | Xiong J.P. Stehle T. Zhang R. Joachimiak A. Frech M. Goodman S.L. Arnaout M.A. |
| Title | Crystal structure of the extracellular segment of integrin alpha Vbeta3 in complex with an Arg-Gly-Asp ligand. | |
| Source | Science 296:151-155(2002). | |
| PubMed ID | 11884718 | |
| DOI | 10.1126/science.1069040 |
| 4 | Authors | Xiong J.P. Stehle T. Diefenbach B. Zhang R. Dunker R. Scott D.L. Joachimiak A. Goodman S.L. Arnaout M.A. |
| Title | Crystal structure of the extracellular segment of integrin alpha Vbeta3. | |
| Source | Science 294:339-345(2001). | |
| PubMed ID | 11546839 | |
| DOI | 10.1126/science.1064535 |
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