Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC51483B5 domain profile
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PURL: https://purl.expasy.org/prosite/documentation/PDOC51483
Aminoacyl-tRNA synthetases (aaRSs) play a crucial role in the translation of the genetic code by means of covalent attachment of amino acids to their cognate tRNAs. Phenylalanine-tRNA synthetase (PheRS) is known to be among the most complex enzymes of the aaRS family. It is a heterodimeric class II enzyme of the (αβ)2 type. The αβ heterodimer of Thermus thermophilus is composed of ten structural domains: two of them A1-A2 belong to the α subunit and eight B1-B8 to the β subunit. The B5 domain is formed by three α-helices and three antiparallel β-strands and comprises a surface-exposed "winged" helix-turn-helix (HTH) motif (see <PDB:1PYS>) [1,2,3]. The B5 domain has been shown to bind DNA [4].
The profile we developed covers the entire B5 domain.
Last update:March 2010 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Mosyak L. Reshetnikova L. Goldgur Y. Delarue M. Safro M.G. |
| Title | Structure of phenylalanyl-tRNA synthetase from Thermus thermophilus. | |
| Source | Nat. Struct. Biol. 2:537-547(1995). | |
| PubMed ID | 7664121 |
| 2 | Authors | Rodova M. Ankilova V. Safro M.G. |
| Title | Human phenylalanyl-tRNA synthetase: cloning, characterization of the deduced amino acid sequences in terms of the structural domains and coordinately regulated expression of the alpha and beta subunits in chronic myeloid leukemia cells. | |
| Source | Biochem. Biophys. Res. Commun. 255:765-773(1999). | |
| PubMed ID | 10049785 | |
| DOI | 10.1006/bbrc.1999.0141 |
| 3 | Authors | Moor N. Lavrik O. Favre A. Safro M. |
| Title | Prokaryotic and eukaryotic tetrameric phenylalanyl-tRNA synthetases display conservation of the binding mode of the tRNA(Phe) CCA end. | |
| Source | Biochemistry 42:10697-10708(2003). | |
| PubMed ID | 12962494 | |
| DOI | 10.1021/bi034732q |
| 4 | Authors | Dou X. Limmer S. Kreutzer R. |
| Title | DNA-binding of phenylalanyl-tRNA synthetase is accompanied by loop formation of the double-stranded DNA. | |
| Source | J. Mol. Biol. 305:451-458(2001). | |
| PubMed ID | 11152603 | |
| DOI | 10.1006/jmbi.2000.4312 |
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