PROSITE documentation PDOC51494SpoIVB peptidase domain profile
The protein SpoIVB is a serine peptidase that plays a central role in a regulatory checkpoint (the sigma-K checkpoint) which coordinates gene expression during the later stages of spore formation in Bacillus subtilis. SpoIVB signal by transiting a membrane, undergoing self cleavage, and then by an unknown mechanism activating a zinc metalloprotease, SpoIVFB, which cleaves pro-sigma-K to its active form, sigma-K, in the outer mother cell chamber of the developing cell [E1]. Homologues of SpoIVB proteins have been identified in a number of spore-forming organisms. SpoIVB carries a single PDZ domain (see <PDOC50106>) and a 160-amino-acid residue catalytic serine peptidase domain at the carboxyl terminus of the protein. His, Asp and Ser residues form the catalytic triad of the SpoIVB serine peptidase domain [1,2]. The SpoIVB peptidase domain forms peptidase family S55 of clan PA(S) [2] [E1].
For SpoIVB to cleave another SpoIVB molecule in trans it must target a specific region at the COOH terminus of the SpoIVB peptidase domain. This region carries a Thr-His-Val motif identical to the classical PDZ recognition motif ([ST]-X-V) normally found at the extreme COOH terminus of PDZ-recognized proteins [2].
The profile we developed covers the entire SpoIVB peptidase domain.
Last update:May 2011 / Text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Hoa N.T. Brannigan J.A. Cutting S.M. |
| Title | The Bacillus subtilis signaling protein SpoIVB defines a new family of serine peptidases. | |
| Source | J. Bacteriol. 184:191-199(2002). | |
| PubMed ID | 11741860 |
| 2 | Authors | Dong T.C. Cutting S.M. |
| Title | The PDZ domain of the SpoIVB transmembrane signaling protein enables cis-trans interactions involving multiple partners leading to the activation of the pro-sigmaK processing complex in Bacillus subtilis. | |
| Source | J. Biol. Chem. 279:43468-43478(2004). | |
| PubMed ID | 15292188 | |
| DOI | 10.1074/jbc.M407048200 |
| E1 | Title | https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=s55 |
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