PROSITE documentation PDOC51521
Herpesvirus tegument ubiquitin-specific protease (htUSP) domain profile


All herpesviruses [E1] contain a Ubiquitin (Ub)-specific cysteine protease (USP) domain embedded within their large tegument protein. The herpesvirus tegument ubiquitin (Ub)-specific protease (htUSP) domain of ~200 amino acids adopts an α-β-α sandwich fold that features a central catalytic cleft, ideally suited to accommodate the C-terminal stretch of Ub (see <PDB:2J7Q>). The catalytic triad Cys-His-Asp is strictly conserved, along with a putative oxyanion hole-forming Gln residue. The htUSP domain is a member of peptidase family C76 of clan CA [1,2,3,4,5,E2].

The profile we developed covers the entire htUSP domain.

Last update:

January 2011 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

HTUSP, PS51521; Herpesvirus tegument ubiquitin-specific protease (htUSP) domain profile  (MATRIX)


1AuthorsSchlieker C. Korbel G.A. Kattenhorn L.M. Ploegh H.L.
TitleA deubiquitinating activity is conserved in the large tegument protein of the herpesviridae.
SourceJ. Virol. 79:15582-15585(2005).
PubMed ID16306630

2AuthorsSchlieker C. Weihofen W.A. Frijns E. Kattenhorn L.M. Gaudet R. Ploegh H.L.
TitleStructure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes.
SourceMol. Cell 25:677-687(2007).
PubMed ID17349955

3AuthorsGredmark S. Schlieker C. Quesada V. Spooner E. Ploegh H.L.
TitleA functional ubiquitin-specific protease embedded in the large tegument protein (ORF64) of murine gammaherpesvirus 68 is active during the course of infection.
SourceJ. Virol. 81:10300-10309(2007).
PubMed ID17634221

4AuthorsGonzalez C.M. Wang L. Damania B.
TitleKaposi's sarcoma-associated herpesvirus encodes a viral deubiquitinase.
SourceJ. Virol. 83:10224-10233(2009).
PubMed ID19640989

5AuthorsKim E.T. Oh S.E. Lee Y.-O. Gibson W. Ahn J.-H.
TitleCleavage specificity of the UL48 deubiquitinating protease activity of human cytomegalovirus and the growth of an active-site mutant virus in cultured cells.
SourceJ. Virol. 83:12046-12056(2009).
PubMed ID19759126



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