|PROSITE documentation PDOC51526|
The RFX family of transcription factors is characterized by a unique ~75-residue DNA-binding domain. RFX genes have been isolated in yeasts, nematode and vertebrates. The characteristic RFX-type HTH DNA binding domain has been recruited into otherwise very divergent regulatory factors functioning in a diverse spectrum of unrelated systems, including regulation of the mitotic cell cycle in fission yeast, the control of the immune response in mammals, and infection by human hepatitis B virus [1,2].
The RFX-type DNA-binding domain is an unusual member of the winged-helix subfamily of helix-turn-helix domains because it uses a β-hairpin (or wing) to recognize DNA instead of the recognition helix typical of helix-tun-helix domains. It consists of three α-helices (H), three β-strands (S) and three connecting loops (L), arranged in the order H1-S1-H2-L1-H3-L2-S2-W1-S3. The third loop, connecting β-strands S2 and S3, forms wing W1 of the winged-helix motif (see <PDB:1DP7>). In contrast to previously described winged-helix DBDs (typically 110 residues long with two wings, W1 and W2), the shorter RFX-type winged-helix DNA-binding domain has only one wing .
Some proteins known to contain a RFX-type winged-helix DNA-binding domain are listed below:
The profile we developed covers the entire RFX-type winged-helix DNA-binding domain.Last update:
March 2011 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Emery P. Durand B. Mach B. Reith W.|
|Title||RFX proteins, a novel family of DNA binding proteins conserved in the eukaryotic kingdom.|
|Source||Nucleic Acids Res. 24:803-807(1996).|
|2||Authors||Durand B. Vandaele C. Spencer D. Pantalacci S. Couble P.|
|Title||Cloning and characterization of dRFX, the Drosophila member of the RFX family of transcription factors.|
|3||Authors||Gajiwala K.S. Chen H. Cornille F. Roques B.P. Reith W. Mach B. Burley S.K.|
|Title||Structure of the winged-helix protein hRFX1 reveals a new mode of DNA binding.|
|4||Authors||Patsialou A. Wilsker D. Moran E.|
|Title||DNA-binding properties of ARID family proteins.|
|Source||Nucleic Acids Res. 33:66-80(2005).|