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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE documentation PDOC51820
PA14 domain profile


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PURL: https://purl.expasy.org/prosite/documentation/PDOC51820

Description

The ~150-amino acid PA14 domain, named after its location in the protective antigen of anthrax toxin, is found in a variety of bacterial and eukaryotic proteins such as glycosidases, glycosyltransferases, proteases, amidases, toxins, adhesins and signaling molecules. The PA14 domain is combined in a mosaic manner with various catalytic or non-catalytic domains directly or indirectly implicated in binding carbohydrate or peptidoglycan. It could be a carbohydrate-binding module [1,2,3].

The PA14 domain is a β-barrel structure comprising two β-sheets (see <PDB:1ACC>). The N and C termini of the domain are close together in space, presumably, thereby facilitating the insertion of the PA14 domain into other recognized domains without structural disruption [1,2,3].

The profile we developed covers the entire PA14 domain.

Last update:

November 2016 / First entry.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

PA14, PS51820; PA14 domain profile  (MATRIX)


References

1AuthorsRigden D.J. Mello L.V. Galperin M.Y.
TitleThe PA14 domain, a conserved all-beta domain in bacterial toxins, enzymes, adhesins and signaling molecules.
SourceTrends Biochem. Sci. 29:335-339(2004).
PubMed ID15236739
DOI10.1016/j.tibs.2004.05.002

2AuthorsYoshida E. Hidaka M. Fushinobu S. Koyanagi T. Minami H. Tamaki H. Kitaoka M. Katayama T. Kumagai H.
TitleRole of a PA14 domain in determining substrate specificity of a glycoside hydrolase family 3 beta-glucosidase from Kluyveromyces marxianus.
SourceBiochem. J. 431:39-49(2010).
PubMed ID20662765
DOI10.1042/BJ20100351

3AuthorsPetosa C. Collier R.J. Klimpel K.R. Leppla S.H. Liddington R.C.
TitleCrystal structure of the anthrax toxin protective antigen.
SourceNature 385:833-838(1997).
PubMed ID9039918
DOI10.1038/385833a0



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