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PROSITE documentation PDOC52008 |
Glycoside hydrolases (GHs, EC 3.2.1.-) form a widespread group of enzymes that hydrolyze the glycoside bond between monosaccharide units or between a carbohydrate and an aglycone moiety. GHs can act specifically as exo-cleaving enzymes to remove the sugar units from the ends of chains and release small sugar products, or endo-cleaving enzymes to act within the polysaccharide chain and produce oligosaccharides. The GH family 81 (GH81) includes proteins with endo-β-1,3-glucanase widely distributed in plants, fungi, bacteria, archaea and viruses. GH81 proteins show rather diverse physiological roles. These proteins share a common region of around 650 amino acids that is characteristic of the family [1,2,3,4].
The core catalytic domain of family 81 GHs consists of an N-terminal β-sandwich subdomain, a C-terminal (α/β)6 barrel subdomain and an additional α/β-subdomain between them (see <PDB:4K3A>). The β-sandwich subdomain compacts with (α/β)6 barrel subdomain and forms a long binding cleft. Along the catalytic cleft there are a number of aromatic residues that are conserved in GH family 81 glucanases and may be involved in stacking interactions with the rings of the glucosyl residues of the substrate. Three amino acid residues that are conserved in the family have been proposed as candidate catalytic residues: an aspartate (acid catalyst) and two glutamates (proton donor and basic catalyst respectively). These residues are located in the base of the catalytic cleft present near the center of the (α/β)6 barrel [2,3,4].
Some proteins known to contain a GH81 domain are listed below:
The profile we developed covers the whole GH81 domain.
Last update:October 2022 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
1 | Authors | Martin-Cuadrado A.-B. Fontaine T. Esteban P.-F. del Dedo J.E. de Medina-Redondo M. del Rey F. Latge J.P. Vazquez de Aldana C.R. |
Title | Characterization of the endo-beta-1,3-glucanase activity of S. cerevisiae Eng2 and other members of the GH81 family. | |
Source | Fungal. Genet. Biol. 45:542-553(2008). | |
PubMed ID | 17933563 | |
DOI | 10.1016/j.fgb.2007.09.001 |
2 | Authors | Zhou P. Chen Z. Yan Q. Yang S. Hilgenfeld R. Jiang Z. |
Title | The structure of a glycoside hydrolase family 81 endo-beta-1,3-glucanase. | |
Source | Acta Crystallogr. D. Biol. Crystallogr. 69:2027-2038(2013). | |
PubMed ID | 24100321 | |
DOI | 10.1107/S090744491301799X |
3 | Authors | Pluvinage B. Fillo A. Massel P. Boraston A.B. |
Title | Structural Analysis of a Family 81 Glycoside Hydrolase Implicates Its Recognition of beta-1,3-Glucan Quaternary Structure. | |
Source | Structure 25:1348-1359.e3(2017). | |
PubMed ID | 28781080 | |
DOI | 10.1016/j.str.2017.06.019 |
4 | Authors | Kumar K. Correia M.A.S. Pires V.M.R. Dhillon A. Sharma K. Rajulapati V. Fontes C.M.G.A. Carvalho A.L. Goyal A. |
Title | Novel insights into the degradation of beta-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase. | |
Source | Int. J. Biol. Macromol. 117:890-901(2018). | |
PubMed ID | 29870811 | |
DOI | 10.1016/j.ijbiomac.2018.06.003 |
E1 | Source | http://www.cazy.org/GH81.html |