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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE documentation PDOC52032
MarR-like, BRCT and chromo domains module profile


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PURL: https://purl.expasy.org/prosite/documentation/PDOC52032

Description

Mammalian SWI/SNF (mSWI/SNF) of BAF complexes are a subfamily of SWI/SNF ATP-dependent chromatin remodelers that dynamically modulate chromatin structure to regulate fundamental cellular processes including gene transcription, cell cycle control, and DNA damage response [1]. The BAF155/SMARCC1 subunit is a component of all complexes identified to date, incorporated either as a homodimer or as a heterodimer with its paralog BAF170/SMARCC2. The BAF155 subunit predominates in stem cells, while the BAF170 subunit is incorporated during differentiation. The N-terminus of the BAF155/SMARCC1 and BAF170/ SMARCC2 subunits contain a putative DNA-binding MarR-like domain, a chromo domain (see <PDOC00517>) and a BRCT domain that are interconnected to each other to form a distinct module. This N-terminal module could participate in an interaction involving both protein and nucleic acid components [2].

The MarR-like domain forms a four-helix-bundle that resembles the MarR-like helix-turn-helix domain (see <PDOC00861>). The BRCT domain contains an inserted chromo domain between the second and third strands of the canonical fold. The chromo domain consists of the curved antiparallel three-stranded β-sheet characteristic of this domain. The individual domains are linked via a network of hydrogen bonds to form a single structural unit (see <PDB:6YXO>. The architecture of the fold produces a cleft containing two deep pockets at the interface between the domains [2].

The profile we developed covers the entire MarR-like, BRCT and chromo domains module.

Last update:

July 2023 / First entry.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

MARR_BRCT_CHROMO, PS52032; MarR-like, BRCT and chromo domains module profile  (MATRIX)


References

1AuthorsYan L. Xie S. Du Y. Qian C.
TitleStructural Insights into BAF47 and BAF155 Complex Formation.
SourceJ. Mol. Biol. 429:1650-1660(2017).
PubMed ID28438634
DOI10.1016/j.jmb.2017.04.008

2AuthorsAllen M.D. Freund S.M.V. Bycroft M. Zinzalla G.
TitleSWI/SNF subunit BAF155 N-terminus structure informs the impact of cancer-associated mutations and reveals a potential drug binding site.
SourceCommun. Biol. 4:528-528(2021).
PubMed ID33953332
DOI10.1038/s42003-021-02050-z



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