PROSITE documentation PDOC60000Chitosanases families 46 and 80 active sites signature
Chitosanases (EC 3.2.1.132) are enzymes that catalyze the hydrolysis of chitosan, but not chitin. They are defined as carbohydrolases that hydrolyze partially N-acetylated chitosan, splitting the β-1,4-glycosidic linkages except the N-acetyl-glucosamine homo-linkages (GlcNAc-GlcNAc) [1,E1]. From the view point of sequence and folding similarities chitosanases belong to families 46, 75 or 80 in the classification of glycosyl hydrolases [2,E2]. Chitosanases of families 46 and 80 are enzymes from bacteria and Chlorella viruses that share a conserved active site-related module [3]. This module includes several amino acids essential for enzyme activity and (or) stability as shown by site-directed mutagenesis studies.
Expert(s) to contact by email: Last update:May 2004 / Text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Fukamizo T. Brzezinski R. |
| Title | Chitosanase from Streptomyces sp. strain N174: a comparative review of its structure and function. | |
| Source | Biochem. Cell Biol. 75:687-696(1997). | |
| PubMed ID | 9599657 |
| 2 | Authors | Henrissat B. Bairoch A. |
| Title | Updating the sequence-based classification of glycosyl hydrolases. | |
| Source | Biochem. J. 316:695-696(1996). | |
| PubMed ID | 8687420 |
| 3 | Authors | Tremblay H. Blanchard J. Brzezinski R. |
| Title | A common molecular signature unifies the chitosanases belonging to families 46 and 80 of glycoside hydrolases. | |
| Source | Can. J. Microbiol. 46:952-955(2000). | |
| PubMed ID | 11068683 |
| E1 | Source | http://rbrzezinski.recherche.usherbrooke.ca |
| E2 | Source | http://www.cazy.org/fam/acc_GH.html |
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