PROSITE documentation PDOC60000Chitosanases families 46 and 80 active sites signature
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PURL: https://purl.expasy.org/prosite/documentation/PDOC60000
Chitosanases (EC 3.2.1.132) are enzymes that catalyze the hydrolysis of chitosan, but not chitin. They are defined as carbohydrolases that hydrolyze partially N-acetylated chitosan, splitting the β-1,4-glycosidic linkages except the N-acetyl-glucosamine homo-linkages (GlcNAc-GlcNAc) [1]. From the view point of sequence and folding similarities chitosanases belong to families 46, 75 or 80 in the classification of glycosyl hydrolases [2,E1]. Chitosanases of families 46 and 80 are enzymes from bacteria and Chlorella viruses that share a conserved active site-related module [3]. This module includes several amino acids essential for enzyme activity and (or) stability as shown by site-directed mutagenesis studies.
Last update:May 2004 / Text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Fukamizo T. Brzezinski R. |
| Title | Chitosanase from Streptomyces sp. strain N174: a comparative review of its structure and function. | |
| Source | Biochem. Cell Biol. 75:687-696(1997). | |
| PubMed ID | 9599657 |
| 2 | Authors | Henrissat B. Bairoch A. |
| Title | Updating the sequence-based classification of glycosyl hydrolases. | |
| Source | Biochem. J. 316:695-696(1996). | |
| PubMed ID | 8687420 |
| 3 | Authors | Tremblay H. Blanchard J. Brzezinski R. |
| Title | A common molecular signature unifies the chitosanases belonging to families 46 and 80 of glycoside hydrolases. | |
| Source | Can. J. Microbiol. 46:952-955(2000). | |
| PubMed ID | 11068683 |
| E1 | Title | https://www.cazy.org/Glycoside-Hydrolases.html |
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