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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE entry PS00099


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PURL: https://purl.expasy.org/prosite/signature/PS00099

Direct ScanProsite submission
General information about the entry

Entry name [info] THIOLASE_3
Accession [info] PS00099
Entry type [info] PATTERN
Date [info] 01-APR-1990 CREATED;
01-APR-2006 DATA UPDATE;
29-MAY-2024 INFO UPDATE.
PROSITE Doc. [info] PDOC00092
Associated ProRule [info] PRU10020

Name and characterization of the entry

Description [info] Thiolases active site.
Pattern [info] 
[AG]-[LIVMA]-[STAGCLIVM]-[STAG]-[LIVMA]-C-{Q}-[AG]-x-[AG]-x-[AG]-x-[SAG].

Numerical results [info]

Numerical results for UniProtKB/Swiss-Prot release 2025_04 which contains 573'661 sequence entries.


Total number of hits 301 in 301 different sequences
Number of true positive hits 260 in 260 different sequences
Number of 'unknown' hits 0
Number of false positive hits 41 in 41 different sequences
Number of false negative sequences 50
Number of 'partial' sequences 3
Precision (true positives / (true positives + false positives)) 86.38 %
Recall (true positives / (true positives + false negatives)) 83.87 %

Comments [info]

Taxonomic range [info] Archaea, Eukaryotes, Prokaryotes (Bacteria)
Maximum number of repetitions [info] 1
Site [info] active_site at position 6
Version [info] 2

Cross-references [info]

UniProtKB/Swiss-Prot
True positive sequences		 260 sequences 

AACT1_ARATH (Q9FIK7), ACCT2_ARATH (Q8S4Y1), ATOB_ECOLI  (P76461), 

ATOB_HAEIN  (P44873), ATOB_PSEAE  (Q9I2A8), BKTB_CUPNH  (Q0KBP1), 
CATF_ACIAD  (Q43935), ECHB_BOVIN  (O46629), ECHB_HUMAN  (P55084), 
ECHB_MACFA  (Q8HXX4), ECHB_MOUSE  (Q99JY0), ECHB_PANTR  (Q5R1W7), 
ECHB_RAT(Q60587), ECHH_CAEEL  (P34255), ER10A_ASPFC (B0XMC1), 
ER10A_ASPFU (Q4WLA8), ER10A_GIBZE (I1RMA2), ER10B_GIBZE (I1RY81), 
ERG10_SACMO (P10551), ERG10_SCHPO (Q9UQW6), ERG10_YEAST (P41338), 
FADA4_MYCBO (P66927), FADA4_MYCLE (P46707), FADA4_MYCS2 (A0R1Y7), 
FADA4_MYCTO (P9WG68), FADA4_MYCTU (P9WG69), FADA_ACIAD  (Q6FF69), 
FADA_ACIB3  (B7H1I1), FADA_ACIB5  (B7I3P0), FADA_ACIBC  (B2I2J8), 
FADA_ACIBS  (B0VLX5), FADA_ACIBT  (A3M1H9), FADA_ACIBY  (B0VE46), 
FADA_AERHH  (A0KEL0), FADA_AERS4  (A4STF3), FADA_ALCBS  (Q0VNZ7), 
FADA_ALIF1  (Q5E8X7), FADA_ALIFM  (B5FEW7), FADA_ALISL  (B6EGU1), 
FADA_BACSU  (O32177), FADA_CHRSD  (Q1QUW9), FADA_CITK8  (A8ACZ3), 
FADA_COLP3  (Q489W4), FADA_CROS8  (A7MQM5), FADA_ECO24  (A7ZU50), 
FADA_ECO57  (Q8X8J4), FADA_ECOHS  (A8A6V0), FADA_ECOK1  (A1AI32), 
FADA_ECOL5  (Q0TAL1), FADA_ECOL6  (Q8FBI3), FADA_ECOLI  (P21151), 
FADA_ECOUT  (Q1R467), FADA_ENT38  (A4WFX5), FADA_ENTCL  (Q9F0Y6), 
FADA_ERWT9  (B2VFE0), FADA_ESCF3  (B7LTZ0), FADA_HAHCH  (Q2SD23), 
FADA_IDILO  (Q5QXH8), FADA_KLEP7  (A6TGM3), FADA_MARMS  (A6VVM8), 
FADA_MARN8  (A1TZR8), FADA_PECAS  (Q6DAP6), FADA_PECCP  (C6DI66), 
FADA_PHOLL  (Q7MZ91), FADA_PHOPR  (Q6LW07), FADA_PSE14  (Q48GW4), 
FADA_PSEA6  (Q15ZF4), FADA_PSEF5  (Q4KFC3), FADA_PSEFR  (P28790), 
FADA_PSEPF  (Q3K9D9), FADA_PSESM  (Q87ZB3), FADA_PSET1  (Q3IJ24), 
FADA_PSEU2  (Q4ZRA1), FADA_PSYA2  (Q4FQC7), FADA_PSYCK  (Q1Q8K0), 
FADA_PSYWF  (A5WH98), FADA_SALCH  (Q57HM7), FADA_SALPA  (Q5PKQ3), 
FADA_SALTI  (P0A2H8), FADA_SALTY  (P0A2H7), FADA_SERP5  (A8G8D0), 
FADA_SHEAM  (A1S1I7), FADA_SHEB5  (A3CYJ3), FADA_SHEB8  (A6WH99), 
FADA_SHEDO  (Q12TB5), FADA_SHEFN  (Q08A40), FADA_SHELP  (A3Q8U3), 
FADA_SHEON  (Q8EKS0), FADA_SHEPC  (A4Y1B5), FADA_SHESA  (A0KR49), 
FADA_SHESM  (Q0HPB8), FADA_SHESR  (Q0I0T4), FADA_SHESW  (A1RDW3), 
FADA_SHIBS  (Q31UE3), FADA_SHIDS  (Q32A20), FADA_SHIF8  (Q0SZ35), 
FADA_SHIFL  (Q83PG2), FADA_SHISS  (Q3YVC2), FADA_STUS1  (A4VKA4), 
FADA_VIBC3  (A5F464), FADA_VIBCH  (Q9KNI0), FADA_VIBPA  (Q87TP0), 
FADA_VIBVU  (Q8DDK5), FADA_VIBVY  (Q7MQI0), FADA_YERE8  (A1JIG3), 
FADA_YERP3  (A7FDF1), FADA_YERPA  (Q1C2C3), FADA_YERPE  (Q8ZAM9), 
FADA_YERPN  (Q1CNA0), FADA_YERPP  (A4TR28), FADA_YERPS  (Q66FR9), 
FADI_ALTMD  (B4RTU9), FADI_CITK8  (A8ADP1), FADI_COLP3  (Q47ZB6), 
FADI_CROS8  (A7MH80), FADI_ECO24  (A7ZPF9), FADI_ECO27  (B7UFZ9), 
FADI_ECO45  (B7MGV8), FADI_ECO55  (B7LBJ6), FADI_ECO57  (Q8XCN9), 
FADI_ECO5E  (B5YXY5), FADI_ECO7I  (B7NP25), FADI_ECO81  (B7MY17), 
FADI_ECO8A  (B7M6M3), FADI_ECOBW  (C4ZVN3), FADI_ECODH  (B1X9L5), 
FADI_ECOHS  (A8A2L1), FADI_ECOK1  (A1ADI9), FADI_ECOL5  (Q0TFA5), 
FADI_ECOL6  (Q8FFG3), FADI_ECOLC  (B1IXA4), FADI_ECOLI  (P76503), 
FADI_ECOLU  (B7N5V3), FADI_ECOSE  (B6I6Q5), FADI_ECOSM  (B1LME8), 
FADI_ECOUT  (Q1R971), FADI_ENT38  (A4WCW7), FADI_ERWT9  (B2VJ10), 
FADI_ESCF3  (B7LLC9), FADI_IDILO  (Q5QXN5), FADI_KLEP3  (B5XVW1), 
FADI_KLEP7  (A6TC20), FADI_PECAS  (Q6D2L6), FADI_PECCP  (C6DAL8), 
FADI_PHOLL  (Q7N287), FADI_PSEA6  (Q15VA3), FADI_PSET1  (Q3IEE3), 
FADI_SALA4  (B5EZS0), FADI_SALAR  (A9MJ36), FADI_SALCH  (Q57LW5), 
FADI_SALDC  (B5FPN2), FADI_SALEP  (B5R3S0), FADI_SALHS  (B4TCA9), 
FADI_SALNS  (B4SZR1), FADI_SALPA  (Q5PCX7), FADI_SALPB  (A9N452), 
FADI_SALPC  (C0PZX5), FADI_SALPK  (B5BBA0), FADI_SALSV  (B4TQC3), 
FADI_SALTI  (Q8Z4Y9), FADI_SALTY  (Q8ZNA6), FADI_SERP5  (A8GH87), 
FADI_SHEAM  (A1S7L7), FADI_SHEB2  (B8EE97), FADI_SHEB5  (A3D685), 
FADI_SHEB8  (A6WQ26), FADI_SHEB9  (A9KTW7), FADI_SHEDO  (Q12P12), 
FADI_SHEFN  (Q07ZP7), FADI_SHEHH  (B0TL22), FADI_SHELP  (A3QFP4), 
FADI_SHEON  (Q8ECP6), FADI_SHEPA  (A8H5T2), FADI_SHEPC  (A4Y898), 
FADI_SHEPW  (B8CPY7), FADI_SHESA  (A0KV75), FADI_SHESH  (A8FTR6), 
FADI_SHESM  (Q0HKD2), FADI_SHESR  (Q0HWN4), FADI_SHESW  (A1RI91), 
FADI_SHEWM  (B1KKT1), FADI_SHIB3  (B2TWV5), FADI_SHIBS  (Q31YB6), 
FADI_SHIDS  (Q32DJ3), FADI_SHIF8  (Q0T2E5), FADI_SHIFL  (Q83K95), 
FADI_SHISS  (Q3YZM1), FADI_YERE8  (A1JK23), FADI_YERP3  (A7FGK0), 
FADI_YERPA  (Q1C659), FADI_YERPB  (B2K8J6), FADI_YERPE  (Q8ZD46), 
FADI_YERPG  (A9R7W9), FADI_YERPN  (Q1CHK1), FADI_YERPP  (A4TM83), 
FADI_YERPS  (Q668V0), FADI_YERPY  (B1JGG1), KAT1_PETHY  (C8YNG6), 
PAAJ_ECOLI  (P0C7L2), PAAJ_ECOLX  (P0C7L3), PCAF_ACIAD  (Q43974), 
PCAF_PSEAE  (Q9I6R0), PCAF_PSEKB  (Q8VPF1), PCAF_PSEPU  (Q51956), 
THIA_CANTR  (Q12598), THIB_CANTR  (Q04677), THIC_HUMAN  (Q9BWD1), 
THIC_MOUSE  (Q8CAY6), THIC_RAT(Q5XI22), THIK1_ARATH (Q8LF48), 
THIK2_ARATH (Q56WD9), THIK5_ARATH (Q570C8), THIKA_CANTR (P33290), 
THIKA_MOUSE (Q921H8), THIKA_RAT   (P21775), THIKB_CANTR (P33291), 
THIKB_MOUSE (Q8VCH0), THIKB_RAT   (P07871), THIK_ENCCU  (Q8SVA6), 
THIK_HUMAN  (P09110), THIK_YARLI  (Q05493), THIK_YEAST  (P27796), 
THILA_XENLA (Q6NU46), THILB_XENLA (Q6GN02), THIL_ALLVD  (P45369), 
THIL_BOVIN  (Q29RZ0), THIL_CHRVO  (Q9ZHI1), THIL_CUPNH  (P14611), 
THIL_DANRE  (Q6AZA0), THIL_HUMAN  (P24752), THIL_MACFA  (Q8HXY6), 
THIL_MOUSE  (Q8QZT1), THIL_PARDE  (P54810), THIL_RAT(P17764), 
THIL_RHIME  (P50174), THIL_SHIZO  (P07097), THIL_SYNY3  (P73825), 
THIL_THIVI  (P45363), THIL_XENTR  (Q5BKN8), THIM_BOVIN  (Q3T0R7), 
THIM_HUMAN  (P42765), THIM_MOUSE  (Q8BWT1), THIM_PONAB  (Q5RES5), 
THIM_RAT(P13437), THLA_CLOAB  (P45359), THLA_STAA3  (Q2FJQ9), 
THLA_STAA8  (Q2G124), THLA_STAAB  (Q2YVF5), THLA_STAAC  (Q5HIU0), 
THLA_STAAM  (Q99WM3), THLA_STAAN  (Q7A7L2), THLA_STAAR  (Q6GJW4), 
THLA_STAAS  (Q6GCB8), THLA_STAAW  (Q8NY95), THLA_STAEQ  (Q5HS07), 
THLA_STAES  (Q8CQN7), THLA_SYNWW  (Q0AVM3), THL_BACSU   (P45855), 
Y0859_MYCTU (O53871), YQEF_ECOLI  (Q46939)
» more

UniProtKB/Swiss-Prot
False negative sequences		 50 sequences 

BBSB_GEOMG  (Q39VG1), BBSB_THAAR  (Q9KJF3), ER10B_ASPFC (B0YA65), 

ER10B_ASPFU (Q4WCL5), ERG10_CANAL (A0A1D8PH52), FADA5_MYCTU (I6XHI4), 
FADA6_MYCTU (I6XHJ3), FADA_ECTM1  (A4XSM9), FADA_ECTOL  (Q93Q11), 
FADA_PSEAB  (Q02PH7), FADA_PSEAE  (Q9HZJ3), FADA_PSEE4  (Q1I7D5), 
FADA_PSEP1  (A5W6G9), FADA_PSEP7  (A6V383), FADA_PSEPK  (Q88L01), 
FADA_PSEPU  (Q9R9W0), FADA_SACD2  (Q21KB1), FADI_AERHH  (A0KK76), 
FADI_AERS4  (A4SMT9), FADI_ALIF1  (Q5E3U0), FADI_ALIFM  (B5FGB5), 
FADI_PHOPR  (Q6LTK4), FADI_VIBC3  (A5F2P1), FADI_VIBCH  (Q9KT59), 
FADI_VIBPA  (Q87MM2), FADI_VIBVU  (Q8DB48), FADI_VIBVY  (Q7MIS4), 
SCP2_BOVIN  (P07857), SCP2_CHICK  (Q07598), SCP2_HUMAN  (P22307), 
SCP2_MOUSE  (P32020), SCP2_RABIT  (O62742), SCP2_RAT(P11915), 
THIL1_DICDI (Q86AD9), THILH_CAEEL (Q22100), THIL_YARLI  (Q6L8K7), 
THIOL_METJA (Q58944), THIOL_METTH (O26884), THIOL_METTL (A0A384E138), 
VRAB_STAA1  (Q9KWK4), VRAB_STAAC  (Q5HIA0), VRAB_STAAM  (Q7A2W9), 
VRAB_STAAN  (Q7A768), VRAB_STAAR  (Q6GJ93), VRAB_STAAS  (Q6GBR1), 
VRAB_STAAW  (Q7A1P9), VRAB_STAEQ  (Q5HRH3), VRAB_STAES  (Q8CTR0), 
VRAB_STAHJ  (Q4L3Q1), YHFS_BACSU  (O07618)
» more

UniProtKB/Swiss-Prot
'Partial' sequences		 3 sequences 

THIL_PIG(P14610), THLA_CLODI  (P45362), THLA_CLOPA  (P81347)


				
	

	

		UniProtKB/Swiss-Prot
False positive sequences		
		
			41 sequences
			


			
ACON_BOVIN  (P20004), ACON_CAEEL  (P34455), ACON_HUMAN  (Q99798), 

			
ACON_MOUSE  (Q99KI0), ACON_PIG(P16276), ACON_RAT(Q9ER34), 
ARGJ_OSTLU  (A4S1K1), FINC_CHICK  (P11722), J1I_HCMVA   (P09711), 
LUZ_ARMOS   (A0A3G9K3N1), MYCN_MOUSE  (P03966), MYST1_ORYSJ (Q8LI34), 
PER1_VOLCA  (P81131), SSTT_ALIF1  (Q5DZC3), SSTT_CAMFF  (A0RNW2), 
SSTT_DESPS  (Q6AS08), SSTT_ENTFA  (Q82ZN5), SSTT_PHOPR  (Q6LG82), 
SSTT_PSYIN  (A1SS94), SSTT_SHEAM  (A1S7P9), SSTT_SHELP  (A3QCX7), 
SSTT_SHEON  (Q8ECL5), SSTT_SHESA  (A0KV41), SSTT_SHESM  (Q0HKG6), 
SSTT_SHESR  (Q0HWR8), SSTT_SHESW  (A1RI59), SSTT_STRP1  (Q9A1E0), 
SSTT_STRP3  (P0DF78), SSTT_STRP6  (Q5XDS5), SSTT_STRP8  (Q8P2K3), 
SSTT_STRPB  (Q1JDG4), SSTT_STRPC  (Q1JND7), SSTT_STRPD  (Q1JII6), 
SSTT_STRPF  (Q1J8E1), SSTT_STRPG  (A2RGC2), SSTT_STRPM  (Q48V75), 
SSTT_STRPQ  (P0DF79), SSTT_VIBCH  (Q9KNC9), SSTT_VIBPA  (Q87FP8), 
SSTT_VIBVU  (Q8D4Q1), SSTT_VIBVY  (Q7MGA3)


			
			» more
			


				
	

	

		PDB
[Detailed view]		
		
			107 PDB
			


			pdb_00001afw; pdb_00001dlu; pdb_00001dlv; pdb_00001dm3; pdb_00001m1o; pdb_00001m1t; pdb_00001m3k; pdb_00001m3z; pdb_00001m4s; pdb_00001m4t; pdb_00001nl7; pdb_00001ou6; 
			
				pdb_00001pxt; pdb_00001qfl; pdb_00001wdk; pdb_00001wdl; pdb_00001wdm; pdb_00001wl4; pdb_00001wl5; pdb_00002c7y; pdb_00002c7z; pdb_00002d3t; pdb_00002f2s; pdb_00002ib7; 
				pdb_00002ib8; pdb_00002ib9; pdb_00002ibu; pdb_00002ibw; pdb_00002iby; pdb_00002iik; pdb_00002vtz; pdb_00002vu0; pdb_00002vu1; pdb_00002vu2; pdb_00002wkt; pdb_00002wku; 
				pdb_00002wkv; pdb_00002wl4; pdb_00002wl5; pdb_00002wl6; pdb_00002wu9; pdb_00003goa; pdb_00004b3h; pdb_00004b3i; pdb_00004b3j; pdb_00004c2j; pdb_00004c2k; pdb_00004nzs; 
				pdb_00004o99; pdb_00004o9a; pdb_00004o9c; pdb_00004w61; pdb_00004wyr; pdb_00004wys; pdb_00004xl2; pdb_00005f0v; pdb_00005f38; pdb_00005lp7; pdb_00005xyj; pdb_00005xz5; 
				pdb_00005zqz; pdb_00005zrv; pdb_00006dv2; pdb_00006l2c; pdb_00006l2g; pdb_00007lbz; pdb_00007lca; pdb_00007lcl; pdb_00007ld2; pdb_00007ldc; pdb_00007ldt; pdb_00007ldu; 
				pdb_00007ldv; pdb_00007ldw; pdb_00007o1g; pdb_00007o1i; pdb_00007o1j; pdb_00007o1k; pdb_00007o1l; pdb_00007o1m; pdb_00007o4q; pdb_00007o4r; pdb_00007o4s; pdb_00007o4t; 
				pdb_00007o4v; pdb_00008bnr; pdb_00008bnu; pdb_00008brj; pdb_00008gqh; pdb_00008gqj; pdb_00008opu; pdb_00008opv; pdb_00008opw; pdb_00008opx; pdb_00008opy; pdb_00008oql; 
				pdb_00008oqm; pdb_00008oqn; pdb_00008oqo; pdb_00008oqp; pdb_00008oqq; pdb_00008oqr; pdb_00008oqs; pdb_00008oqt; pdb_00008oqu; pdb_00008oqv; pdb_00008pf8
			
			

			» more