PROSITE logo
Black ribbon
We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE entry PS00191


View entry in original PROSITE format
View entry in raw text format (no links)
View entry auxiliary file in raw text format (no links)
PURL: https://purl.expasy.org/prosite/signature/PS00191

Direct ScanProsite submission
General information about the entry

Entry name [info] CYTOCHROME_B5_1
Accession [info] PS00191
Entry type [info] PATTERN
Date [info] 01-APR-1990 CREATED;
01-DEC-2004 DATA UPDATE;
29-MAY-2024 INFO UPDATE.
PROSITE Doc. [info] PDOC00170

Name and characterization of the entry

Description [info] Cytochrome b5 family, heme-binding domain signature.
Pattern [info] 
[FY]-[LIVMK]-{I}-{Q}-H-P-[GA]-G.

Numerical results [info]

Numerical results for UniProtKB/Swiss-Prot release 2025_04 which contains 573'661 sequence entries.


Total number of hits 126 in 126 different sequences
Number of true positive hits 105 in 105 different sequences
Number of 'unknown' hits 0
Number of false positive hits 21 in 21 different sequences
Number of false negative sequences 80
Number of 'partial' sequences 2
Precision (true positives / (true positives + false positives)) 83.33 %
Recall (true positives / (true positives + false negatives)) 56.76 %

Comments [info]

Taxonomic range [info] Eukaryotes, Prokaryotes (Bacteria), Eukaryotic viruses
Maximum number of repetitions [info] 1
Site [info] heme_iron at position 5
Version [info] 1

Cross-references [info]

UniProtKB/Swiss-Prot
True positive sequences		 105 sequences 

ACO1_AJECA  (Q12618), ACO1_YEAST  (P21147), CYB2_TALSN  (B8MKR3), 

CYB2_WICAO  (P09437), CYB2_YEAST  (P00175), CYB52_SCHPO (Q9USM6), 
CYB5A_ARATH (Q9FDW8), CYB5B_ARATH (O48845), CYB5B_HUMAN (O43169), 
CYB5B_MOUSE (Q9CQX2), CYB5B_PONAB (Q5RDJ5), CYB5B_RAT   (P04166), 
CYB5C_ARATH (Q9ZNV4), CYB5D_ARATH (Q9ZWT2), CYB5E_ARATH (Q42342), 
CYB5L_MIMIV (Q5UR80), CYB5L_NEUCR (Q8X0J4), CYB5R_DROME (P19967), 
CYB5R_DROVI (P50266), CYB5S_TOBAC (P49099), CYB5_ALOSE  (P00168), 
CYB5_BOROF  (O04354), CYB5_BOVIN  (P00171), CYB5_BRAOB  (P40934), 
CYB5_CANTR  (Q874I5), CYB5_CHICK  (P00174), CYB5_CUSRE  (P49097), 
CYB5_DROME  (Q9V4N3), CYB5_HORSE  (P00170), CYB5_HUMAN  (P00167), 
CYB5_MORAP  (Q9Y706), CYB5_MOUSE  (P56395), CYB5_MUSDO  (P49096), 
CYB5_NEUCR  (Q9P5L0), CYB5_ORYSJ  (P49100), CYB5_PHATC  (B7GCG7), 
CYB5_PIG(P00172), CYB5_RABIT  (P00169), CYB5_RAT(P00173), 
CYB5_RHIST  (Q9HFV1), CYB5_TOBAC  (P49098), CYB5_YEAST  (P40312), 
CYBL_RHOGR  (P32953), CYP5F_ARATH (O22704), D4FAD_CHLRE (I2CYZ4), 
D4FAD_EUGGR (Q6WNG7), D5FAD_MORAP (O74212), D5FAD_REBSA (A4KDP0), 
DESAT_THAPS (Q4G2T3), DES_CHLRE   (Q2HWK7), FA2H_HUMAN  (Q7L5A8), 
FA2H_MACFA  (Q4R4P4), FA2H_MOUSE  (Q5MPP0), FA2H_RAT(Q2LAM0), 
FAD5C_DICDI (Q1ZXQ5), IRC21_YEAST (Q04772), NB5R4_BOVIN (Q32LH7), 
NB5R4_DANRE (Q502I6), NB5R4_HUMAN (Q7L1T6), NB5R4_MOUSE (Q3TDX8), 
NB5R4_RAT   (Q68EJ0), NB5R4_XENTR (Q28CZ9), NIA1_ARATH  (P11832), 
NIA1_BRANA  (P39867), NIA1_HORVU  (P27967), NIA1_MAIZE  (P17571), 
NIA1_ORYSJ  (P16081), NIA1_PHAVU  (P39865), NIA1_SOYBN  (P54233), 
NIA1_TOBAC  (P11605), NIA2_ARATH  (P11035), NIA2_BRANA  (P39868), 
NIA2_HORVU  (P27969), NIA2_PHAVU  (P39866), NIA2_SOYBN  (P39870), 
NIA2_TOBAC  (P08509), NIA3_MAIZE  (P49102), NIA7_HORVU  (P27968), 
NIA_ASPNG   (P36858), NIA_BEABA   (P43100), NIA_BETPN   (P27783), 
NIA_CHLVU   (Q01170), NIA_CICIN   (P43101), NIA_CUCMA   (P17569), 
NIA_EMENI   (P22945), NIA_FUSOX   (P39863), NIA_LEPMC   (P36842), 
NIA_LOTJA   (P39869), NIA_MYCMD   (Q05531), NIA_NEUCR   (P08619), 
NIA_PETHY   (P36859), NIA_PHYIN   (P39864), NIA_PICAN   (P49050), 
NIA_SOLLC   (P17570), NIA_SPIOL   (P23312), NIA_ULVPR   (A0A286R227), 
NIA_VOLCA   (P36841), RLF_ARATH   (Q9LXD1), SCS7_YEAST  (Q03529), 
SUOX_CHICK  (P07850), SUOX_DROME  (Q9VWP4), SUOX_HUMAN  (P51687), 
SUOX_MOUSE  (Q8R086), SUOX_RAT(Q07116), YDAA_SCHPO  (Q10352)
» more

UniProtKB/Swiss-Prot
False negative sequences		 80 sequences 

ACET6_CERPU (Q9LEN0), ACO1_SCHPO  (O94523), APF12_GIBF5 (S0DLN7), 

CB5D1_DANRE (Q567I9), CB5D1_HUMAN (Q6P9G0), CB5D1_MOUSE (Q5NCY3), 
CB5D1_PONAB (Q5R4Q5), CB5D1_XENLA (Q5PPR6), CB5D1_XENTR (Q0IHR1), 
CHS5B_FUSO4 (A0A0J9UR47), CYB5_ECTSH  (P82291), D4FAD_DIALT (Q6VPV2), 
D4FAD_REBSA (A0PJ29), D4FAD_THRSP (Q8S3C0), D5FAD_PHYPA (A9SIZ6), 
D5FAD_THRSP (Q8S3C1), D8FAD_REBSA (A4KDP1), DAP1_SCHPO  (O13995), 
DAP1_YEAST  (Q12091), DES6_CERPU  (Q9LEM9), FAD5A_DICDI (Q9Y1W0), 
FAD5B_DICDI (O96099), FADS1_HUMAN (O60427), FADS1_MOUSE (Q920L1), 
FADS1_PAPAN (A4UVI1), FADS1_RAT   (Q920R3), FADS2_BOVIN (A4FV48), 
FADS2_DANRE (Q9DEX7), FADS2_HUMAN (O95864), FADS2_MACFA (Q4R749), 
FADS2_MOUSE (Q9Z0R9), FADS2_PONAB (Q5REA7), FADS2_RAT   (Q9Z122), 
FADS2_TACFU (A0A0C5PRW9), FADS3_HUMAN (Q9Y5Q0), FADS3_MOUSE (Q9JJE7), 
FADS3_RAT   (Q8K1P9), FAT3_CAEEL  (Q23221), FAT4_CAEEL  (G5EG11), 
HERC2_HUMAN (O95714), HERC2_MOUSE (Q4U2R1), MAPR4_ARATH (Q2HIW2), 
MSBP1_ARATH (Q9XFM6), MSBP1_ORYSJ (Q9FVZ7), MSBP2_ARATH (Q9M2Z4), 
MSBP2_ORYSJ (Q9FVZ9), NENF_BOVIN  (Q1JQA5), NENF_HUMAN  (Q9UMX5), 
NENF_MOUSE  (Q9CQ45), NENF_RAT(Q6IUR5), NEUFC_CAEBR (Q60YT6), 
NEUFC_CAEEL (Q9XXA7), NEUFC_DANRE (A2CES0), NEUFC_DROME (Q9W376), 
NEUFC_DROPS (Q29HF1), NEUFC_HUMAN (Q8WUJ1), NEUFC_MOUSE (Q5SSH8), 
NEUFC_RAT   (Q6AY62), NEUFC_XENTR (Q28FI8), PGRC1_BOVIN (Q17QC0), 
PGRC1_CHICK (Q5ZKN2), PGRC1_HUMAN (O00264), PGRC1_MOUSE (O55022), 
PGRC1_PIG   (Q95250), PGRC1_PONAB (Q5RED0), PGRC1_RAT   (P70580), 
PGRC2_HUMAN (O15173), PGRC2_MOUSE (Q80UU9), PGRC2_RAT   (Q5XIU9), 
SBP3_ARATH  (Q9SK39), SLD1_ARATH  (Q9ZRP7), SLD1_BOROF  (Q9FR82), 
SLD1_EUGGR  (Q9SWQ9), SLD1_HELAN  (Q43469), SLD1_KLULA  (Q6CMK7), 
SLD1_KLULC  (Q8NKG8), SLD1_LACK1  (Q8NKG9), SLD2_ARATH  (Q3EBF7), 
SUOX_MACFA  (Q60HD0), VEM1_CAEEL  (Q7YZW5)
» more

UniProtKB/Swiss-Prot
'Partial' sequences		 2 sequences 

NIA2_MAIZE  (P39871), NIA_LOTTE   (P39882)


				
	

	

		UniProtKB/Swiss-Prot
False positive sequences		
		
			21 sequences
			


			
CCD32_MOUSE (Q8BS39), CCD32_RAT   (Q561K4), CDK14_CALJA (B0VXE8), 

			
CDK14_DASNO (C0RW22), CDK14_HUMAN (O94921), CDK14_MOUSE (O35495), 
CDK14_PLEMO (B0VXL7), CDK14_RABIT (B6A7Q3), CDK14_XENLA (Q6DJM7), 
CDK14_XENTR (A4IIW7), HYAL3_PIG   (Q6RHW2), LAR1B_HUMAN (Q659C4), 
LARP1_HUMAN (Q6PKG0), LARP1_MOUSE (Q6ZQ58), MATK_WOLBI  (Q6JVN2), 
MSRAB_HELPJ (Q9ZMK8), PPCEL_XENLA (Q32N48), SFSA_METS4  (B0ULY8), 
VCLY_TEGPF  (P07447), VILI_BOVIN  (Q3SZP7), VILL_HUMAN  (O15195)


			
			» more
			


				
	

	

		PDB
[Detailed view]		
		
			70 PDB
			


			pdb_00001aqa; pdb_00001aw3; pdb_00001awp; pdb_00001axx; pdb_00001b5a; pdb_00001b5b; pdb_00001b5m; pdb_00001bfx; pdb_00001blv; pdb_00001cyo; pdb_00001do9; pdb_00001ehb; 
			
				pdb_00001es1; pdb_00001eue; pdb_00001f03; pdb_00001f04; pdb_00001fcb; pdb_00001hko; pdb_00001i5u; pdb_00001i87; pdb_00001i8c; pdb_00001ib7; pdb_00001icc; pdb_00001iet; 
				pdb_00001ieu; pdb_00001j0q; pdb_00001jex; pdb_00001kbi; pdb_00001lco; pdb_00001ldc; pdb_00001lj0; pdb_00001lqx; pdb_00001lr6; pdb_00001ltd; pdb_00001m20; pdb_00001m2i; 
				pdb_00001m2m; pdb_00001mj4; pdb_00001mny; pdb_00001nx7; pdb_00001sh4; pdb_00001sox; pdb_00001sze; pdb_00001szf; pdb_00001szg; pdb_00001u9m; pdb_00001u9u; pdb_00002axx; 
				pdb_00002i89; pdb_00002i96; pdb_00002ibj; pdb_00002m33; pdb_00002oz0; pdb_00003hbg; pdb_00003hbp; pdb_00003hbq; pdb_00003ks0; pdb_00003lf5; pdb_00003mus; pdb_00003ner; 
				pdb_00003ozz; pdb_00003r18; pdb_00003r19; pdb_00003x32; pdb_00003x33; pdb_00003x34; pdb_00003x35; pdb_00004hil; pdb_00004hin; pdb_00004zr0
			
			

			» more