We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.

PROSITE entry PS50006

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PURL: https://purl.expasy.org/prosite/signature/PS50006

Direct ScanProsite submission

General information about the entry

Entry name [info]	FHA_DOMAIN
Accession [info]	PS50006
Entry type [info]	MATRIX
Date [info]	01-NOV-1995 CREATED; 26-FEB-2020 DATA UPDATE; 29-MAY-2024 INFO UPDATE.
PROSITE Doc. [info]	PDOC50006
Associated ProRule [info]	PRU00086

Name and characterization of the entry

Description [info]

Forkhead-associated (FHA) domain profile.

Matrix / Profile [info]

/GENERAL_SPEC: ALPHABET='ABCDEFGHIKLMNPQRSTVWYZ'; LENGTH=53; TOPOLOGY=LINEAR;
/DISJOINT: DEFINITION=PROTECT; N1=6; N2=48;
/NORMALIZATION: MODE=1; FUNCTION=LINEAR; R1=0.8473000; R2=0.0187000; TEXT='NScore';
/NORMALIZATION: MODE=-1; FUNCTION=LINEAR; R1=2393.4443359; R2=1.1111112; PRIORITY=1; TEXT='Heuristic 5.0%';
/CUT_OFF: LEVEL=1; SCORE=410; H_SCORE=2849; N_SCORE=8.5; MODE=1; TEXT='!!';
/CUT_OFF: LEVEL=0; SCORE=330; H_SCORE=2760; N_SCORE=7.0; MODE=1; TEXT='!';
/CUT_OFF: LEVEL=-1; SCORE=276; H_SCORE=2700; N_SCORE=6.0; MODE=1; TEXT='?';
/DEFAULT: B1=-50; E1=-50; MI=-105; MD=-105; IM=-105; DM=-105; I=-20; D=-20;

...

                A   B   C   D   E   F   G   H   I   K   L   M   N   P   Q   R   S   T   V   W   Y   Z
/I:         B1=0; BI=-105; BD=-105;
/M: SY='V';  M= -6,-26,-15,-30,-25,  5,-29,-24, 16,-19,  7,  5,-24,-26,-22,-19,-16, -6, 18, -6,  3,-24;
/M: SY='T';  M= -8,-12,-22,-16,-11, -6,-19,-10, -6, -2, -7, -3, -6,-18, -8,  3, -2,  5, -2,-21, -3,-11;
/M: SY='I';  M=-10,-29,-24,-35,-27, 16,-30,-25, 28,-29, 22, 13,-22,-25,-25,-24,-20,-10, 19,-15,  3,-27;
/M: SY='G';  M=  0,-10,-30,-10,-20,-30, 70,-20,-40,-20,-30,-20,  0,-20,-20,-20,  0,-20,-30,-20,-30,-20;
/M: SY='R';  M=-19, -8,-30, -8,  1,-21,-20,  5,-30, 29,-21, -9,  1,-19, 10, 62,-10,-11,-21,-21, -8,  1;
/M: SY='N';  M= -6,  4,-23, -2, -6,-16, -7,  1,-15, -3,-21,-12, 15,-10, -3,  2,  4, -3,-16,-27, -9, -7;
/M: SY='S';  M=  3, -1,-18, -4, -2,-18,-14,-14,-12, -3,-17,-12,  1, -8, -4, -5, 11, 11, -5,-30,-15, -4;
/M: SY='D';  M= -6, 16,-25, 24, 17,-29,-12, -7,-28,  3,-26,-21,  6,  1,  2, -6,  7,  1,-22,-34,-19,  9;
/I:         MI=-12; MD=-12; IM=-12; I=-4;
/M: SY='R'; D=-2;  M= -6,  1,-17,  0,  3,-16, -5, -2,-14,  4,-13, -8,  6, -6,  6,  7,  1, -4,-15,-19,-11,  4;
/I:         DM=-12;
/M: SY='C';  M= -4,-20, 45,-25,-23,-14,-22,-26, -9,-22,-10, -9,-18,-26,-23,-21, -6, -5,  5,-38,-21,-24;
/M: SY='D';  M=-18, 42,-29, 56, 20,-38,-10,  2,-36,  1,-29,-26, 21,-11,  7, -7,  1, -9,-30,-38,-19, 13;
/M: SY='V';  M= -5,-27,-10,-29,-25, -1,-26,-23, 20,-22, 15, 10,-25,-28,-21,-20,-15, -6, 25,-24, -5,-24;
/M: SY='A';  M=  2, -3,-19, -6, -7,-12,-14, -6,-10, -4,-11, -8,  0,-17, -7, -3,  2,  0, -5,-25, -7, -8;
/I:         MD=-10;
/M: SY='L'; D=-3;  M= -2,-11,-13,-11, -6, -3, -9, -5,  1,-12, 11,  6,-10,-14, -6, -9, -7, -4, -1,-15, -4, -6;
/I:         MI=0; IM=0; DM=-10; I=-5;
/M: SY='L';  M= -6,-21,-17,-25,-20,  3, -2,-19, -2,-23,  7,  4,-16,-24,-17,-19,-14,-12, -4,-17, -6,-19;
/M: SY='E';  M=-10,  9,-28, 12, 16,-27,-11, -5,-25,  6,-22,-16,  7,  1,  5,  0,  0, -5,-24,-30,-18, 10;
/M: SY='S';  M=  0,  5,-22,  6,  0,-23, -2, -5,-20,-10,-21,-16,  7, -3, -4,-12, 10, -1,-17,-33,-18, -3;
/M: SY='P';  M= -8, -5,-29, -4,  4,-26,-11, -9,-23, 10,-26,-12, -2, 15,  3,  5, -1, -6,-22,-27,-19,  2;
/M: SY='V';  M= -8,-11,-19,-12,-12,  1,-21,-15,  0,-10, -3, -3, -8,-22,-14, -4, -4, -3,  6,-24, -6,-13;
/M: SY='I';  M= -7,-30,-22,-35,-28,  2,-35,-28, 38,-27, 22, 17,-25,-25,-23,-25,-19, -7, 33,-23, -3,-28;
/M: SY='S';  M= 10,  0,-10,  0,  0,-20,  0,-10,-20,-10,-30,-20, 10,-10,  0,-10, 40, 20,-10,-40,-20,  0;
/M: SY='R';  M=-14, -4,-27, -6, -2,-21, -8, -3,-29, 20,-22,-12,  6,-19,  5, 46, -4, -6,-21,-23,-13, -2;
/M: SY='R';  M=-10, -9,-15,-14,-11, -6,-22,-10, -5,  2, -9, -2, -4,-22, -7,  4, -8, -4,  1,-22, -4,-10;
/M: SY='H';  M=-20,  0,-30,  0,  0,-20,-20,100,-30,-10,-20,  0, 10,-20, 10,  0,-10,-20,-30,-30, 20,  0;
/M: SY='A';  M= 23,-15, 21,-24,-18,-10, -9,-23,-14,-18, -9,-11,-14,-21,-19,-23,  0, -5, -3,-25,-18,-18;
/M: SY='Q';  M= -6, -7,-14, -8,  4,-17,-21, -9,-12, -2,-10, -6, -7,-16,  7,  0, -5, -7,-10,-24,-11,  5;
/M: SY='I';  M=-10,-30,-23,-36,-28, 13,-35,-26, 33,-29, 25, 16,-24,-25,-24,-25,-21, -9, 24,-17,  3,-28;
/M: SY='Y';  M=-11,-10,-23,-13, -5,  3,-20,  5,-16, -5,-11, -7, -5,-18, -4,  0, -3, -1,-14,-10,  6, -5;
/M: SY='Y';  M=-10,-21, -6,-24,-19,  8,-27, -5, -2,-15,  2,  0,-19,-27,-14,-14,-16,-10, -2, -3, 19,-18;
/M: SY='D';  M=-10, 11,-26, 12,  6,-24,  0, -1,-23, -3,-18,-14,  9,-15,  0, -6, -2, -7,-21,-30,-16,  3;
/I:         MD=-10;
/M: SY='P'; D=-3;  M=-10, -5,-22, -6, -2, -3,-15, -3,-12, -7,-10, -6, -2,  6, -5, -5, -6, -6,-15,-19, -6, -4;
/I:         MD=-10;
/M: SY='Q'; D=-3;  M= -8,  0,-16, -2,  0,-10,-10,  2,-12, -2, -7, -3,  1,-11,  4,  1, -2,  0,-11,-16, -4,  2;
/I:         MI=0; IM=0; DM=-10; I=-5;
/M: SY='D';  M= -7,  4,-23,  6,  5,-22, -9,-10,-18, -2,-18,-13,  3,-13,  0, -2,  5,  2,-13,-30,-16,  2;
/M: SY='G';  M= -7, -1,-30, -3, -4,-22, 10,-11,-27, -5,-26,-17,  4,  0, -5, -7, -4,-12,-27,-15,-18, -5;
/M: SY='R';  M= -4, -2,-24, -5,  4,-20, -8, -2,-20,  7,-16, -8,  2,-15,  1, 10, -2, -6,-15,-25,-13,  1;
/M: SY='W';  M=-10,-28,-27,-31,-24, 15,-26,-17,  4,-20,  1, -1,-25,-27,-19,-18,-20,-12,  4, 27, 14,-20;
/M: SY='Y';  M=-10,-10,-25,-10, -3,  3,-16,  3,-10, -9, -7, -6, -6,-19, -6, -9, -5, -6,-12,-10, 15, -6;
/M: SY='L';  M= -8,-30,-21,-33,-24,  6,-33,-24, 30,-28, 35, 18,-27,-27,-22,-23,-24, -8, 22,-22, -2,-24;
/M: SY='H';  M=-11,  1,-27,  2, 11,-19,-19, 14,-21,  5,-18, -9,  3,-14,  8,  8, -4, -9,-19,-26, -6,  8;
/M: SY='D';  M=-16, 27,-14, 37,  8,-30,-17, -7,-23, -7,-21,-20, 10,-15, -6,-14, -3, -9,-17,-38,-18,  0;
/I:         MI=0; IM=0; I=-8;
/M: SY='A';  M= -8,-14,-18,-17,-14, -8, -5, -7,-10,-10, -6, -3, -8,-20,-13,-10, -8, -8, -8,-22, -7,-14;
/M: SY='S';  M=  7, -3,-16, -3, -6,-23, 20,-13,-26,-13,-30,-20,  7,-13, -6,-13, 29,  9,-16,-34,-23, -6;
/M: SY='T';  M= -2, -4,-17, -7, -1,-16,-15,-11,-15,  2,-14, -8, -1,-13, -1,  5, 11, 14, -8,-29,-12, -1;
/M: SY='N';  M= -9, 32,-20, 16,  1,-21, -2, 13,-21, -1,-29,-18, 50,-19,  4,  0, 11,  0,-28,-38,-17,  2;
/M: SY='G';  M= -1,-12,-29,-11,-20,-28, 59,-21,-35,-19,-28,-18, -3,-15,-20,-20, -1,-18,-25,-21,-29,-20;
/M: SY='T';  M=  6, -5, -4,-13,-11,-11,-17,-20, -9,-12, -9, -9, -4,-13,-11,-13, 16, 32,  1,-31,-13,-11;
/M: SY='F';  M=-16,-22,-25,-26,-18, 29,-28, -9, -1,-13,  6,  1,-18,-26,-16, -8,-18, -8, -5,  7, 25,-17;
/M: SY='V';  M= -5,-29,-17,-32,-26,  3,-31,-25, 30,-24, 25, 18,-28,-28,-23,-21,-19, -5, 32,-25, -5,-26;
/M: SY='N';  M=-11, 38,-21, 25,  5,-23, -3,  7,-23,  0,-29,-21, 49,-17,  1, -2,  9,  1,-29,-39,-20,  3;
/M: SY='G';  M= -8,  2,-26,  0,-10,-12, 21, -1,-28,-11,-23,-16, 10,-20,-12,-11, -1,-12,-24,-21,-10,-11;
/M: SY='Q';  M= -5, -7,-21,-10, -3,-17,-20, -5, -6, -1, -7, -2, -3,-18,  4,  4, -3, -3, -3,-26,-10,  0;
/M: SY='R';  M=-15,-14,-27,-14, -5, -8,-22, -8,-18, 15,-11, -6, -7,-16, -1, 36,-11, -8,-11,-19, -7, -6;
/M: SY='L';  M= -9,-24,-22,-27,-18, -1,-30,-13, 20,-22, 24, 17,-21,-24,-10,-17,-19, -7, 13,-22, -2,-15;
/I:         E1=0; IE=-105; DE=-105;

» more

Numerical results [info]

Numerical results for UniProtKB/Swiss-Prot release 2025_04 which contains 573'661 sequence entries.

Total number of hits	159 in 157 different sequences
Number of true positive hits	154 in 152 different sequences
Number of 'unknown' hits	0
Number of false positive hits	5 in 5 different sequences
Number of false negative sequences	39
Number of 'partial' sequences	0

Precision (true positives / (true positives + false positives))	96.86 %
Recall (true positives / (true positives + false negatives))	79.79 %

Comments [info]

Taxonomic range [info]	Eukaryotes, Prokaryotes (Bacteria)
Maximum number of repetitions [info]	2
Matrix type [info]	protein_domain
Scaling database [info]	reversed
Author [info]	K_Hofmann
Feature key [info]	DOMAIN
Feature description [info]	FHA
Version [info]	7

Cross-references [info]

UniProtKB/Swiss-Prot True positive sequences	152 sequences ABA2_NICPL (Q40412), ABA2_PRUAR (O81360), ABA2_SOLLC (P93236), ABA2_SPIOL (A0A0K9RDW0), ABC1_MYCTU (O65934), AGGF1_HUMAN (Q8N302), AGGF1_MOUSE (Q7TN31), C170B_HUMAN (Q9Y4F5), C170B_MOUSE (Q80U49), C170B_XENLA (Q498L0), C170B_XENTR (A0JM08), CE170_HUMAN (Q5SW79), CE170_MOUSE (Q6A065), CHFR_DANRE (A5WW08), CHFR_HUMAN (Q96EP1), CHFR_MOUSE (Q810L3), CHFR_PONAB (Q5RF77), CHFR_XENLA (Q5FWP4), CHFR_XENTR (Q6P256), CHK2_CAEEL (Q9U1Y5), CHK2_HUMAN (O96017), CHK2_MOUSE (Q9Z265), DDL_ARATH (Q8W4D8), DMA1_SCHPO (Q10322), DMA1_YEAST (P38823), DMA2_YEAS7 (A6ZRW7), DMA2_YEAST (P53924), DUN1_YEAST (P39009), EMBR_MYCBO (P66800), EMBR_MYCTO (P9WGJ8), EMBR_MYCTU (P9WGJ9), FAR10_YEAST (Q06001), FHA1_ARATH (Q9SFV6), FHA1_TOBAC (Q945P0), FHA2_ARATH (Q9SFV2), FHAA_MYCTU (P71590), FHAB_MYCS2 (A0QNG6), FHAB_MYCTO (P9WJB4), FHAB_MYCTU (P9WJB5), FHAD1_HUMAN (B1AJZ9), FHAD1_MOUSE (A6PWD2), FHKA_DICDI (Q54BF0), FHKB_DICDI (Q1ZXH2), FHKC_DICDI (P34101), FHKD_DICDI (Q54MH0), FHKE_DICDI (Q54VI1), FKH1_YEAST (P40466), FKH2_CANAL (Q5A7S7), FKH2_SCHPO (O60129), FKH2_YEAST (P41813), FKHL1_CANAL (Q59ZC8), FKHL1_SCHPO (O14270), FKHL1_YEAST (P39521), FORJ_DICDI (Q54ER5), FOXK1_HUMAN (P85037), FOXK1_MOUSE (P42128), FOXK2_HUMAN (Q01167), FOXK2_MOUSE (Q3UCQ1), FOXK2_XENLA (Q7ZX03), FRAH_NOSS1 (P46017), GARA_MYCS2 (A0QYG2), GARA_MYCTO (P9WJA8), GARA_MYCTU (P9WJA9), KI67_HUMAN (P46013), KI67_MOUSE (E9PVX6), KIF14_MOUSE (L0N7N1), KIF1A_HUMAN (Q12756), KIF1A_MOUSE (P33173), KIF1A_RAT (F1M4A4), KIF1B_HUMAN (O60333), KIF1B_MOUSE (Q60575), KIF1B_RAT (O88658), LOK_DROME (O61267), MCRS1_HUMAN (Q96EZ8), MCRS1_MOUSE (Q99L90), MDC1_HUMAN (Q14676), MDC1_MACMU (Q5TM68), MDC1_MOUSE (Q5PSV9), MDC1_PANTR (Q7YR40), MDC1_PIG(Q767L8), MDC1_RAT(Q5U2M8), MEK1_SCHPO (Q10292), MEK1_YEAST (P24719), NADAP_HUMAN (Q9BWU0), NBN_CHICK (Q9DE07), NBN_DANRE (Q5I2W8), NBN_HUMAN (O60934), NBN_MOUSE (Q9R207), NBN_PONAB (Q5RCV3), NBN_RAT (Q9JIL9), NBN_XENLA (Q6XV80), NBS1_ARATH (Q0H8D7), NBS1_CHATD (G0SAV1), NBS1_ORYSI (B8BHK8), NBS1_ORYSJ (Q7XD82), NBS1_SCHPO (O43070), ODHI_CORDI (Q6NHD4), ODHI_COREF (Q8FTJ5), ODHI_CORGL (Q8NQJ3), ODHI_CORJK (Q4JVU0), P2C70_ARATH (P46014), PHF12_HUMAN (Q96QT6), PHF12_MOUSE (Q5SPL2), PLM2_YEAST (Q04383), PML1_YEAST (Q07930), PP1R8_ARATH (Q9FIK2), PP1R8_BOVIN (Q28147), PP1R8_HUMAN (Q12972), PP1R8_MOUSE (Q8R3G1), PS1_ARATH (B7SY83), RAD53_SCHPO (Q09170), RAD53_YEAST (P22216), RNF8A_XENLA (Q7ZX20), RNF8B_XENLA (Q6NRG0), RNF8_BOVIN (Q2HJ46), RNF8_DANRE (Q803C1), RNF8_HUMAN (O76064), RNF8_MOUSE (Q8VC56), RNF8_PONAB (Q5R4I2), RNF8_RAT(Q4KLN8), SLMAP_HUMAN (Q14BN4), SLMAP_MOUSE (Q3URD3), SLMAP_RABIT (Q28623), SLMAP_RAT (P0C219), SNIP1_HUMAN (Q8TAD8), SNIP1_MOUSE (Q8BIZ6), SNIP1_RAT (Q5M9G6), STAR9_MOUSE (Q80TF6), TCF19_HUMAN (Q9Y242), TCF19_MACMU (Q5TM48), TCF19_MOUSE (Q99KJ5), TCF19_PANTR (Q7YR48), TCF19_PIG (Q9TSV4), TIFA_BOVIN (A2VDM0), TIFA_HUMAN (Q96CG3), TIFA_MOUSE (Q793I8), TIFA_RAT(Q5XIB9), TIFA_XENLA (Q2MHQ9), TIFA_XENTR (B5DE70), TOS4_YEAST (Q06266), VPS64_YEAST (Q03944), Y1858_MYCBO (P64898), Y1895_SYNY3 (P74101), Y4449_ARATH (O23305), Y8013_DICDI (Q54VU4), YGI1_YEAST (P53156), YK42_SCHPO (Q9P7G6), YKI5_CAEEL (P46012), YNVD_SCHPO (O74388), YOT2_CAEEL (P34648), ZEP_ARATH (Q9FGC7), ZEP_ORYSJ (Q0JCU7) » more Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits: [Clustal format, color, condensed view] [Clustal format, color] [Clustal format, plain text] [Fasta format] Retrieve the sequence logo from the alignment
UniProtKB/Swiss-Prot False negative sequences	39 sequences ABA2_CAPAN (Q96375), AFAD_HUMAN (P55196), AFAD_MOUSE (Q9QZQ1), AFAD_RAT(O35889), KI13A_HUMAN (Q9H1H9), KI13A_MOUSE (Q9EQW7), KI13B_HUMAN (Q9NQT8), KI16B_HUMAN (Q96L93), KI16B_MOUSE (B1AVY7), KIF14_HUMAN (Q15058), KIF1A_AEDAE (Q17BU3), KIF1A_ANOGA (Q7PHR1), KIF1A_DROME (A1ZAJ2), KIF1A_DROPS (Q28WQ1), KIF1C_HUMAN (O43896), KIF1C_MOUSE (O35071), KIF1C_RAT (O35787), KIF1_DICDI (Q9NGQ2), KIF28_HUMAN (B7ZC32), KIF28_MOUSE (D3YXS5), KIF28_RAT (F8WLE0), PELI1_HUMAN (Q96FA3), PELI1_MOUSE (Q8C669), PELI2_HUMAN (Q9HAT8), PELI2_MOUSE (Q8BST6), PHLB1_HUMAN (Q86UU1), PHLB1_MOUSE (Q6PDH0), PNKP_HUMAN (Q96T60), PNKP_MOUSE (Q9JLV6), RADIL_DANRE (A7UA95), RADIL_HUMAN (Q96JH8), RADIL_MOUSE (Q69Z89), STAR9_HUMAN (Q9P2P6), TIFAB_HUMAN (Q6ZNK6), TIFAB_MOUSE (Q8JZM6), TIFAB_RAT (Q5BK67), XRS2_YEAST (P33301), Y7958_DICDI (Q55FT8), ZEP_ONCHC (C3VEQ2) » more
UniProtKB/Swiss-Prot False positive sequences	5 sequences CHSAD_CHOCO (Q0VZ70), SYA_FINM2 (B0S104), SYA_RUTMC (A1AXE0), SYA_STAHJ (Q4L6W5), SYY_RALN1 (Q8Y240) » more
PDB [Detailed view]	107 PDB pdb_00001dmz; pdb_00001fhq; pdb_00001fhr; pdb_00001g3g; pdb_00001g6g; pdb_00001gxc; pdb_00001j4k; pdb_00001j4l; pdb_00001j4o; pdb_00001j4p; pdb_00001j4q; pdb_00001k2m; pdb_00001k2n; pdb_00001k3j; pdb_00001k3n; pdb_00001k3q; pdb_00001lgp; pdb_00001lgq; pdb_00001mzk; pdb_00001qu5; pdb_00001r21; pdb_00001uht; pdb_00002a0t; pdb_00002aff; pdb_00002csw; pdb_00002eh0; pdb_00002fez; pdb_00002ff4; pdb_00002jkd; pdb_00002jpe; pdb_00002jqi; pdb_00002jqj; pdb_00002jql; pdb_00002kb3; pdb_00002kb4; pdb_00002kfu; pdb_00002kkl; pdb_00002lc1; pdb_00002pie; pdb_00003els; pdb_00003elv; pdb_00003hue; pdb_00003huf; pdb_00003i0m; pdb_00003i0n; pdb_00003i6u; pdb_00003i6w; pdb_00003oun; pdb_00003po8; pdb_00003poa; pdb_00003umz; pdb_00003un0; pdb_00003unm; pdb_00003unn; pdb_00003uot; pdb_00003va1; pdb_00003va4; pdb_00003vpy; pdb_00004egx; pdb_00004ejq; pdb_00004h87; pdb_00004jon; pdb_00004qcj; pdb_00004ym4; pdb_00004zgi; pdb_00005gm6; pdb_00005lqw; pdb_00005t2f; pdb_00005t2s; pdb_00005xzv; pdb_00005xzw; pdb_00005yyx; pdb_00005yyz; pdb_00005z56; pdb_00005z57; pdb_00005z58; pdb_00005zwm; pdb_00005zwo; pdb_00006a8w; pdb_00006ar0; pdb_00006ar2; pdb_00006c4u; pdb_00006cah; pdb_00006ccd; pdb_00006ff7; pdb_00006i2p; pdb_00006i2q; pdb_00006i2r; pdb_00006i2s; pdb_00006l9u; pdb_00006l9v; pdb_00006l9w; pdb_00007abg; pdb_00007abh; pdb_00007abi; pdb_00007dco; pdb_00007dvq; pdb_00007rj3; pdb_00007zr1; pdb_00008bah; pdb_00008bpa; pdb_00008c60; pdb_00008i0p; pdb_00008i0r; pdb_00008p5r; pdb_00008p5x; pdb_00008wwy » more