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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE entry PS50027


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PURL: https://purl.expasy.org/prosite/signature/PS50027

Direct ScanProsite submission
General information about the entry

Entry name [info] EGF_LAM_2
Accession [info] PS50027
Entry type [info] MATRIX
Date [info] 01-MAY-2005 CREATED;
01-OCT-2013 DATA UPDATE;
29-MAY-2024 INFO UPDATE.
PROSITE Doc. [info] PDOC00961
Associated ProRule [info] PRU00460

Name and characterization of the entry

Description [info] Laminin-type EGF-like (LE) domain profile.
Matrix / Profile [info] 
/GENERAL_SPEC: ALPHABET='ACDEFGHIKLMNPQRSTVWY'; LENGTH=50;
/DISJOINT: DEFINITION=PROTECT; N1=1; N2=50;
/NORMALIZATION: MODE=1; FUNCTION=LINEAR; R1=3.6474471; R2=0.0190416; TEXT='NScore';
/CUT_OFF: LEVEL=0; SCORE=439; N_SCORE=12.0; MODE=1; TEXT='R';
/CUT_OFF: LEVEL=-1; SCORE=124; N_SCORE=6.0; MODE=1; TEXT='R?';
/DEFAULT: B1=*; E1=*; MM=*; II=*;

...
 
                A   C   D   E   F   G   H   I   K   L   M   N   P   Q   R   S   T   V   W   Y
/I:         B1=-85; E0=*; BD=-50;
/M: SY='C';  M=-78, 59,-79,-83,-83,-68,-75,-93,-84,-88,-87,-81,-73,-83,-78,-83,-81,-89,-71,-83; M0=-79;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='N';  M=-23,-38, 17,  8, -5,-18,  6,-39, -4,-19,-29, 20, -8, 18, -7,  2, -7,-34,-40, -1; M0=-9;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='C';  M=-78, 59,-79,-83,-83,-68,-75,-93,-84,-88,-87,-81,-73,-83,-78,-83,-81,-89,-71,-83; M0=-79;
/I:         MM=0; MI=-102; MD=-50; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='N';  M=-34,-48, 20,-22, -7,-39, 28,-49,-27,-48,-40, 29,  2,-25,-33,  7,-33,-44,-46, -5; M0=-22;
/I:         MM=-2; MI=-101; MD=-28; IM=-9; II=-11; DM=-14; DD=-7;
/M: SY='P';  M=-21,-34,-20, -7,-37,  5,  6,-10,-14,-20,-25, -2, 31,  0,  1,  0,-21,  3,-36,-30; M0=-10;
/I:         MM=-1; MI=-48; MD=-109; IM=-10; II=-10; DM=-19; DD=-5;
/M: SY='H';  M=-22,-34,  9,-16, -7,-32, 22,  2,  2, -1,-25,  8, -4, 11, -9, -3, -5,  9,-36,  9; M0=-5;
/I:         MM=0; MI=-99; MD=-110; IM=-9; II=-11; DM=-1; DD=-34;
/M: SY='G';  M=-53,-56,-61,-64,-76, 38,-66,-81,-72,-80,-74, -6,-63,-68,-71,-56,-58,-70,-70,-76; M0=-57;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='S';  M=  8,-36, -6,-19,-39,-35, 25,-16,-19,-37,-28,  4,-36,-18,-23, 26,  5,-16,-39,-33; M0=-15;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='Y';  M=  3,-30,-29,  3,  3,-36,  8,  3, -9,  8,-22,-25, -8,  9, -7,  6, -1,  3,-34, 10; M0=-5;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='S';  M=-10,-38,  2,  5, -6,  5, 17,-39, -5,-38,-29,  2, -2, -5, -5, 23, -9,-34,-40,-33; M0=-10;
/I:         MM=-1; MI=-102; MD=-44; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='E';  M=  0,-37,  5, 12, -9,  6,-19,-12,  5, -7,-28, -1, -6, -1,  6, 10, -6,-11,-39,-33; M0=-4;
/I:         MM=-2; MI=-101; MD=-29; IM=-9; II=-11; DM=-17; DD=-5;
/M: SY='T';  M= -3,-36,  2, -3,-39,  2,-17, -4, -7, -7,-27, 11,-32,  5,  7, -4, 16,  5,-37, -1; M0=-5;
/I:         MM=-4; MI=-20; MD=-109; IM=-10; II=-10; DM=-1; DD=-36;
/M: SY='C';  M=-78, 59,-79,-83,-83,-68,-75,-93,-84,-88,-87,-81,-73,-83,-78,-83,-81,-89,-71,-83; M0=-79;
/I:         MM=0; MI=-102; MD=-53; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='D';  M= -4,-42, 27,  4, -9,-36, 19,-43,-20,-42,-33, 26,-37,-19,-25,-12,-27,-11,-44,-37; M0=-18;
/I:         MM=-3; MI=-40; MD=-29; IM=-26; II=-3; DM=-13; DD=-7;
/M: SY='P';  M=-21,-36,-20, -3,-39,-31, -3,-36,  7, -5, 16, -2, 22, 10,  6, -4,  4,  4,-38,-31; M0=-8;
/I:         MM=0; MI=-99; MD=-110; IM=-9; II=-11; DM=-3; DD=-23;
/M: SY='E';  M= -5,-38,  5, 15,-41,-10, 11,-17,  2, -8,-29, 11,-34, -1, -6,  2,  2,  6,-40,  5; M0=-5;
/I:         MM=0; MI=-101; MD=-112; IM=-9; II=-11; DM=-4; DD=-20;
/M: SY='T';  M=-26,-26,-46,-40,-26,  1,  4, -7,-37,-25,-19,-37,-42,-34,-37, 18, 29, -1,-31,  4; M0=-18;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='G';  M=-54,-56,-61,-65,-77, 38,-66,-81,-72,-80,-74, -8,-63,-68,-71,-56,-58,-70,-70,-76; M0=-58;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='Q';  M= -4,-38, -6,-17,  3,-33, 11,-38, -4, -6,-29, -5,-34, 37,  0,-11,-23,-34,-40, -6; M0=-13;
/I:         MM=-2; MI=-33; MD=-112; IM=-17; II=-5; DM=-7; DD=-14;
/M: SY='C';  M=-78, 59,-79,-83,-83,-68,-75,-93,-84,-88,-87,-81,-73,-83,-78,-83,-81,-89,-71,-83; M0=-79;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='Q';  M=-11,-37, -4, 13, -6,-33,  1,-11, -8, -8, 17,-20,  6, 17,  6,  3, -3,  2,  7,-33; M0=-5;
/I:         MM=-3; MI=-25; MD=-112; IM=0; II=-54; DM=-7; DD=-14;
/M: SY='C';  M=-78, 59,-79,-83,-83,-68,-75,-93,-84,-88,-87,-81,-73,-83,-78,-83,-81,-89,-71,-83; M0=-79;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='K';  M=-40,-50,-42,-11,-57,-47,-29,-52, 29,-16,-41,-34, -9, 18, 24,-12,-38,-48,-49,-46; M0=-25;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='P';  M=-10,-38,  9, 12,-41,-33, 20,-39, -5,-38,-29, -1, 29,-15,  2,-22, -6,-13,-40,-33; M0=-14;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='G';  M=-49,-53,-38,-41,-26, 30, 23,-57,-47,-18,-51, 26,-56,-44,-49,-48,-50,-55,-33, -5; M0=-33;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='V';  M=  0,-30,-55,-50, 10,-46,-35,-22,-46,-28,-23,-43,-47,-42,-44,-37, 20, 30,-33,  8; M0=-25;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='T';  M=-10,-37, -7, 12,-39,  4,-20,-12, -4,-19, -1,-20,-34, 11,-21,-23, 27, -2,-39,-32; M0=-10;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='G';  M=-78,-68,-78,-82,-84, 39,-75,-94,-84,-88,-87,-80,-73,-82,-78,-82,-81,-89,-71,-84; M0=-74;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='R';  M= -1,-38, -8, -4,  4,-33,-20,-22,  0,  7,-29,-20, 14, 15, 18,-10,  3,-34,-40,-33; M0=-8;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='H';  M= -3,-40,-25,-19,-44,-36, 24,-41, 12,-40,  0,  9,-37, 23, 22,-25,  5,-37,-42,-35; M0=-18;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='C';  M=-78, 59,-79,-83,-83,-68,-75,-93,-84,-88,-87,-81,-73,-83,-78,-83,-81,-89,-71,-83; M0=-79;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='D';  M=-47,-69, 32, 21,-69,-44,-39,-69,-43,-68,-62, 18,-50,  5,-54,-43,-48,-64,-70,-58; M0=-40;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='R';  M=-18,-38, -8,  1,-41,-33,-20,-19,  5, -2,-29,-20,-34, 21, 28,-11,  5,-23,-40,-33; M0=-12;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='C';  M=-78, 59,-79,-83,-83,-68,-75,-93,-84,-88,-87,-81,-73,-83,-78,-83,-81,-89,-71,-83; M0=-79;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='A';  M= 17,-38,  9,  3,-41,-33,-20,-12, 14,  6,-29,-20,-34, 15, -5, -5,-23,-34,-40,-33; M0=-11;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='P';  M=-10,-38,  9,  1,-41,-33,  8,-39, -4,-16,-29,  7, 31,-15,  1, -1,-12,-34,-40,-33; M0=-13;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='G';  M=-36,-41,-32,-11, -3, 34,  3,-39,-36,-41,-35,-34, -2,-34,-40,-37,-11,-38,-36,  5; M0=-24;
/I:         MM=-2; MI=-29; MD=-112; IM=-7; II=-15; DM=-7; DD=-14;
/M: SY='Y';  M=-67,-57,-71,-75, 36,-70, 11,-57,-70,-50,-51,-57,-69,-58,-64,-21, -4,-58,-25, 37; M0=-47;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='Y';  M= -5,-25,-43,-37, 21,-13, 17,-20,-34,-24,-17,-35,-41,  5,-12,-31, -6, -9, 27, 35; M0=-17;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='G';  M=-10,-38, -5, -1,-41, 28, -2,-39,-16, -8,  3, 11,-34,  7,  1,-22,-23,-34,-40,-33; M0=-13;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='F';  M=-26,  0, 17,-40, 20,-41,-30, -8,-37, 13,-16, 12,-41, -7,-36, -2,-15, -2,-29, 11; M0=-13;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='P';  M=  6,-38, -4, -5,-41,  6,-20,-39,-16,-38,-29, -4, 23,  7,  7,  1,  8,-15,-40, -3; M0=-9;
/I:         MM=-1; MI=-102; MD=-36; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='T';  M= -5,-37, -4, -6,-12,-11,-19,-10, -4, -8,-28, -3,  4, 11, 10, 14, 14,-33, 12, -8; M0=-5;
/I:         MM=-1; MI=-100; MD=-44; IM=-9; II=-11; DM=-22; DD=-4;
/M: SY='N';  M=  4,-36, -1,  1,-40,  3,  4,  3,  7,-16,-27, 15,-10,-14,  7,  5,  4,-33,-38, -2; M0=-5;
/I:         MM=-6; MI=-100; MD=-16; IM=-9; II=-11; DM=-26; DD=-3;
/M: SY='G';  M=  3,-23,-24,-19,  2, 18,  4, -4,-17,-11,-15, 15,  8,  3, -2,-20,-18,  7,-27,-22; M0=-6;
/I:         MM=-2; MI=-94; MD=-28; IM=-9; II=-11; DM=-40; DD=-1;
/M: SY='T';  M=  9,-31,-15, -9,-34, 16,  1,-31, 10,-31,-22,-12, 10,  6, -5,-15, 16,-27,-32,-26; M0=-9;
/I:         MM=-12; MI=-8; MD=-102; IM=-10; II=-10; DM=-3; DD=-24;
/M: SY='G';  M= -3,-37,  5,-15,-40, 26,-19,-37,  2,-16,-28, -2,  3,-14, -9, -1, -6, -6,-39,-32; M0=-9;
/I:         MM=0; MI=-100; MD=-111; IM=-9; II=-11; DM=-2; DD=-28;
/M: SY='C';  M=-78, 59,-79,-83,-83,-68,-75,-93,-84,-88,-87,-81,-73,-83,-78,-83,-81,-89,-71,-83; M0=-79;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='Q';  M= -9,-38,-11,  0, -6,-33,  6,  4, 11,-10,-29,-20,-34, 23, 11,-11, 13,-13,-40, -5; M0=-8;
/I:         MM=0; MI=-102; MD=-112; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='P';  M=  1,-38,-12,-17,-41,-33,-20,-38, 13,-18,-29, -9, 32, -6,  3, -9,-23,  1,-40,-33; M0=-13;
/I:         B0=*; E1=-10;
» more

Numerical results [info]

Numerical results for UniProtKB/Swiss-Prot release 2025_04 which contains 573'661 sequence entries.


Total number of hits 610 in 92 different sequences
Number of true positive hits 586 in 89 different sequences
Number of 'unknown' hits 0
Number of false positive hits 24 in 3 different sequences
Number of false negative sequences 6
Number of 'partial' sequences 1
Precision (true positives / (true positives + false positives)) 96.07 %
Recall (true positives / (true positives + false negatives)) 98.99 %

Comments [info]

Taxonomic range [info] Eukaryotes
Maximum number of repetitions [info] 22
Matrix type [info] protein_domain
Scaling database [info] window20
Author [info] N_Hulo
Feature key [info] DOMAIN
Feature description [info] Laminin EGF-like
Version [info] 3

Cross-references [info]

UniProtKB/Swiss-Prot
True positive sequences		 89 sequences 

AGRIN_CHICK (P31696), AGRIN_DIPOM (Q90404), AGRIN_HUMAN (O00468), 

AGRIN_MOUSE (A2ASQ1), AGRIN_RAT   (P25304), ATRN1_HUMAN (Q5VV63), 
ATRN1_MOUSE (Q6A051), ATRN_HUMAN  (O75882), ATRN_MOUSE  (Q9WU60), 
ATRN_RAT(Q99J86), CELR1_HUMAN (Q9NYQ6), CELR1_MOUSE (O35161), 
CELR2_HUMAN (Q9HCU4), CELR2_MOUSE (Q9R0M0), CELR2_RAT   (Q9QYP2), 
CELR3_HUMAN (Q9NYQ7), CELR3_MOUSE (Q91ZI0), CELR3_RAT   (O88278), 
EPI1_CAEEL  (Q21313), LAM2_CAEEL  (Q18823), LAM3_CAEEL  (G5ECE3), 
LAMA1_HUMAN (P25391), LAMA1_MOUSE (P19137), LAMA2_HUMAN (P24043), 
LAMA2_MOUSE (Q60675), LAMA3_HUMAN (Q16787), LAMA3_MOUSE (Q61789), 
LAMA4_HUMAN (Q16363), LAMA4_MOUSE (P97927), LAMA5_HUMAN (O15230), 
LAMA5_MOUSE (Q61001), LAMA_DROME  (Q00174), LAMB1_CHICK (Q01635), 
LAMB1_DROME (P11046), LAMB1_HUMAN (P07942), LAMB1_HYDVU (Q27262), 
LAMB1_MOUSE (P02469), LAMB2_HUMAN (P55268), LAMB2_MOUSE (Q61292), 
LAMB2_RAT   (P15800), LAMB3_HUMAN (Q13751), LAMB3_MOUSE (Q61087), 
LAMB4_DANRE (Q8JHV6), LAMB4_HUMAN (A4D0S4), LAMB_HIRME  (Q25092), 
LAMC1_DANRE (Q1LVF0), LAMC1_DROME (P15215), LAMC1_HUMAN (P11047), 
LAMC1_MOUSE (P02468), LAMC1_XENTR (A0JP86), LAMC2_HORSE (Q8HZI9), 
LAMC2_HUMAN (Q13753), LAMC2_MOUSE (Q61092), LAMC3_HUMAN (Q9Y6N6), 
LAMC3_MOUSE (Q9R0B6), MEGF8_HUMAN (Q7Z7M0), MEGF8_MOUSE (P60882), 
MEGF8_RAT   (Q9QYP0), MEGF9_HUMAN (Q9H1U4), MEGF9_MOUSE (Q8BH27), 
NET1_CHICK  (Q90922), NET1_HUMAN  (O95631), NET1_MOUSE  (O09118), 
NET1_PIG(Q2HXW4), NET1_RAT(Q924Z9), NET3_CHICK  (Q90923), 
NET3_HUMAN  (O00634), NET3_MOUSE  (Q9R1A3), NET4_HUMAN  (Q9HB63), 
NET4_MOUSE  (Q9JI33), NET4_PONAB  (Q5RB89), NET5_HUMAN  (Q8WTR8), 
NET5_MOUSE  (Q3UQ22), NET5_RAT(D3ZT86), NETA_DROME  (Q24567), 
NETB_DROME  (Q24568), NTNG1_HUMAN (Q9Y2I2), NTNG1_MOUSE (Q8R4G0), 
NTNG2_HUMAN (Q96CW9), NTNG2_MOUSE (Q8R4F1), PGBM_HUMAN  (P98160), 
PGBM_MOUSE  (Q05793), STAN_DROME  (Q9V5N8), TAG53_CAEEL (Q19981), 
UNC52_CAEEL (Q06561), UNC6_CAEEL  (P34710), USH2A_HUMAN (O75445), 
USH2A_MOUSE (Q2QI47), USH2A_RAT   (Q8K3K1)
» more

UniProtKB/Swiss-Prot
False negative sequences		 6 sequences 

SIGK_DICDI  (Q6TMJ6), STAB1_HUMAN (Q9NY15), STAB1_MOUSE (Q8R4Y4), 

STAB2_HUMAN (Q8WWQ8), STAB2_MOUSE (Q8R4U0), STAB2_RAT   (Q8CFM6)
» more

UniProtKB/Swiss-Prot
'Partial' sequences		 1 sequence 

LAMBV_CHICK (Q01636)


				
	

	

		UniProtKB/Swiss-Prot
False positive sequences		
		
			3 sequences
			


			
MEG10_DANRE (E9QJQ6), MEG11_HUMAN (A6BM72), MEG11_MOUSE (Q80T91)


				
	

	

		PDB
[Detailed view]		
		
			29 PDB
			


			pdb_00001klo; pdb_00001npe; pdb_00001tle; pdb_00002y38; pdb_00003tbd; pdb_00003zyg; pdb_00003zyi; pdb_00003zyj; pdb_00004aqs; pdb_00004aqt; pdb_00004ove; pdb_00004plm; 
			
				pdb_00004pln; pdb_00004plo; pdb_00004urt; pdb_00004wnx; pdb_00005lf2; pdb_00006fkq; pdb_00007ler; pdb_00007lrf; pdb_00007ndg; pdb_00007ne0; pdb_00007ne1; pdb_00008dmk; 
				pdb_00008edk; pdb_00008s9p; pdb_00008snp; pdb_00008tsm; pdb_00009d77
			
			

			» more