We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.

PROSITE entry PS50844

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PURL: https://purl.expasy.org/prosite/signature/PS50844

Direct ScanProsite submission

General information about the entry

Entry name [info]	AFP_LIKE
Accession [info]	PS50844
Entry type [info]	MATRIX
Date [info]	01-APR-2002 CREATED; 10-MAY-2017 DATA UPDATE; 29-MAY-2024 INFO UPDATE.
PROSITE Doc. [info]	PDOC50844
Associated ProRule [info]	PRU00021

Name and characterization of the entry

Description [info]

Antifreeze protein-like domain profile.

Matrix / Profile [info]

/GENERAL_SPEC: ALPHABET='ABCDEFGHIKLMNPQRSTVWYZ'; LENGTH=60;
/DISJOINT: DEFINITION=PROTECT; N1=6; N2=55;
/NORMALIZATION: MODE=1; FUNCTION=LINEAR; R1=1.3552001; R2=0.0201396; TEXT='NScore';
/NORMALIZATION: MODE=-1; FUNCTION=LINEAR; R1=2922.5690918; R2=2.2025723; PRIORITY=1; TEXT='Heuristic 5.0%';
/CUT_OFF: LEVEL=0; SCORE=455; H_SCORE=3925; N_SCORE=10.5; MODE=1; TEXT='!';
/CUT_OFF: LEVEL=-1; SCORE=256; H_SCORE=3486; N_SCORE=6.5; MODE=1; TEXT='?';
/DEFAULT: M0=-8; D=-20; I=-20; B1=-60; E1=-60; MI=-105; MD=-105; IM=-105; DM=-105;

...

                A   B   C   D   E   F   G   H   I   K   L   M   N   P   Q   R   S   T   V   W   Y   Z
/I:         B1=0; BI=-105; BD=-105;
/M: SY='S';  M=  8, -2,  0, -3, -4,-21,  2,-12,-22,-12,-29,-20,  7,-13, -4,-12, 33, 15,-11,-40,-21, -4;
/M: SY='I';  M= -7,-30,-20,-33,-26,  4,-33,-26, 32,-27, 28, 17,-27,-27,-23,-23,-20, -7, 29,-23, -3,-26;
/M: SY='V';  M= -7,-24,-16,-26,-24, 11,-28,-10, 14,-18,  4,  5,-21,-27,-23,-16,-10,  0, 25,-17,  9,-24;
/M: SY='A';  M= 23,-15,-13,-22,-15, -3,-12,-17, -1,-14, -1, -4,-14,-17,-15,-18,  2,  2,  6,-17, -4,-15;
/M: SY='K';  M= -4, -5,-22,-12, -8,-13,-20,-13, -4,  3, -5, -2,  0,-18, -6,  2, -6,  0, -3,-25,-10, -8;
/M: SY='R';  M= -5, -6,-26, -7,  4,-26,-18,  4,-21, 18,-19, -5, -2,-14, 17, 23, -5, -9,-16,-21, -9,  9;
/M: SY='D';  M= -8,  9,-26, 11,  1,-16,-15, -9,-18, -1,-13,-13,  5, -4, -6, -7, -6, -8,-18,-28,-13, -3;
/M: SY='I';  M=-10,-29,-26,-34,-26,  6,-35,-23, 35,-28, 27, 18,-24,-25,-20,-24,-23, -9, 22,-16,  6,-26;
/M: SY='P';  M= -9, -6,-31, -4,  5,-29,-19, -9,-19, 13,-25,-10, -4, 18, 10,  4, -6, -8,-20,-25,-17,  6;
/M: SY='K';  M= -3, -8,-28, -9,  3,-23,-21,-14,-14, 20,-18, -7, -6, -3,  2, 11, -8, -9,-11,-22,-12,  0;
/M: SY='G';  M= -2, -2,-28, -5,-17,-28, 59,-15,-37,-17,-30,-20, 10,-20,-17,-17,  2,-17,-30,-23,-28,-17;
/M: SY='E';  M= -2,  7,-20,  9, 16,-19,-16, -7,-20, -2,-17,-16,  1, -8,  2, -8,  9, 12,-15,-27, -9,  8;
/M: SY='A';  M=  7,-19,-21,-23,-14, -9,-21,-16,  7,-12,  2,  2,-16,-12, -8,-12, -8, -6,  7,-18, -5,-13;
/M: SY='L';  M=-10,-29,-22,-32,-23, 13,-32,-20, 23,-28, 37, 17,-27,-28,-21,-21,-26, -9, 14,-16,  6,-23;
/M: SY='T';  M=  3,  0,-13, -4, -1,-19,-12,-11,-16, -7,-20,-13,  4,-10,  6, -7, 25, 28, -9,-33,-14,  2;
/M: SY='L';  M= -9,-17,-24,-18, -7, -8,-24,-14,  3, -4,  9,  9,-16,-13, -7, -3,-15, -7,  1,-23, -8, -8;
/M: SY='D';  M= -5, 20,-25, 29, 17,-29,-15, -8,-21, -3,-18,-18,  4,-10, -1,-11, -1, -8,-15,-33,-18,  8;
/M: SY='N';  M= -8, 25,-21, 14, -2,-19, -6,  4,-14, -3,-18, -3, 31,-18, -1, -5,  2, -4,-19,-34,-16, -1;
/M: SY='I';  M= -8,-25,-21,-31,-24,  1,-30,-20, 28,-22, 23, 25,-22,-23,-16,-20,-18, -3, 21,-22, -2,-21;
/M: SY='T';  M=  1,-10,-21,-14, -8,-15,-18,-17,-10,  5,-14, -7, -7,-15, -5,  5,  0,  6, -3,-15, -9, -8;
/M: SY='V';  M=  7,-22,-16,-27,-21,  7,-21,-23, 12,-21,  5,  3,-17,-21,-21,-20, -4, -1, 16,-20, -5,-21;
/M: SY='K';  M= -3,-12,-22,-11,  1,-14,-23,-16, -5,  7, -1, -1,-14,-17, -5,  0,-11, -7,  2,-24,-10, -2;
/M: SY='R';  M=-15,-14,-25,-14, -4, -8,-23, -8,-15, 12,-11, -6, -8,-21, -2, 37,-10, -8, -4,-20, -7, -6;
/M: SY='P';  M= -5,-18,-30,-12, -6,-24,-13,-20,-13,-12,-24,-15,-15, 48,-12,-18, -1, -4,-14,-31,-25,-12;
/M: SY='G';  M= -3, -2,-13, -2,  1,-26, 24,-14,-32,-11,-25,-19,  3,-16, -8,-14,  5, -7,-24,-29,-25, -4;
/I:         I=-5; MD=-25;
/M: SY='P';  M=  0, -9,-16, -6, -1,-13, -9, -8, -7, -5, -8, -6, -9, 24, -1, -8, -4, -4,-10,-13,-11, -3; D=-5;
/I:         I=-5; MI=0; MD=-25; IM=0; DM=-25;
/M: SY='K';  M= -3, -3,-22, -8, -4,-13,-10, -8,-16,  6,-14, -4,  3,-16,  0,  5, -1, -1,-13,-23,-10, -2;
/M: SY='G';  M=  0,-10,-30,-10,-20,-30, 70,-20,-40,-20,-30,-20,  0,-20,-20,-20,  0,-20,-30,-20,-30,-20;
/M: SY='I';  M=-10,-26,-25,-29,-18,  3,-33,-21, 28,-24, 24, 14,-22,-23,-17,-22,-21, -9, 17,-19,  3,-20;
/M: SY='P';  M=  6, -9,-25, -8,  1,-24,-13, -3,-19, -9,-22,-14, -7, 27, -4,-14,  4, -1,-18,-29,-18, -4;
/M: SY='P';  M=  9,-14,-26,-13, -5,-24,-14,-20,-15,-10,-21,-15,-14, 45,-10,-18,  1,  3,-16,-27,-24,-10;
/M: SY='E';  M= -4,  1,-25,  4, 25,-26,-16, -4,-24, 14,-21,-10,  0, -7, 15,  8,  3, -5,-20,-27,-15, 20;
/M: SY='E';  M=-16,  7,-28, 11, 13,  0,-20,  9,-21, -5,-14,-13,  1,-15, -2, -7, -7,-10,-21,-15,  9,  5;
/M: SY='F';  M= -9,-29,-27,-35,-26, 19,-29,-22, 16,-26, 15,  7,-24,-25,-22,-22,-22,-12,  6, 13, 13,-24;
/M: SY='D';  M=-10,  7,-26, 12,  7,-16,-15, -6,-21, -4,-18,-16,  3,  2, -3, -8,  0, -4,-20,-27,-10,  1;
/M: SY='N';  M=-12,  9,-25,  6,  1,-16,-17, -6,-11,  1, -6, -7, 11,-18, -3,  1, -8, -7,-14,-29,-12, -2;
/M: SY='I';  M= -9,-30,-21,-34,-26, 10,-33,-26, 31,-28, 28, 16,-26,-27,-24,-23,-21, -8, 25,-20,  0,-26;
/M: SY='V';  M= -5,-22,-15,-24,-22,  1,-27,-22, 19,-21, 21, 10,-20,-28,-22,-18,-14, -1, 25,-27, -7,-22;
/I:         I=-6; MI=0; MD=-32; IM=0; DM=-32;
/M: SY='G';  M=  3, -6,-28, -8,-18,-28, 59,-18,-36,-18,-29,-19,  4,-19,-18,-18,  2,-17,-28,-22,-28,-18;
/M: SY='K';  M= -3,-10,-26,-12, -3,-20,-11,-11,-18, 11,-18, -4, -7,-16,  5,  8, -5, -8,-13, -6, -9,  1;
/M: SY='K';  M=-11, -4,-28, -3, 15,-24,-22, -8,-18, 21,-18, -7, -4,-12, 10, 18, -8, -9,-13,-23,-12, 11;
/M: SY='L';  M= 11,-23,-16,-28,-19, -2,-21,-22, 14,-22, 22,  9,-23,-23,-18,-21,-13, -5, 14,-22, -8,-19;
/M: SY='Q';  M= -1, -4,-20, -8,  0,-20,-16, -7,-11,  0,-10, -5,  0,-16, 10,  2,  3,  1,-10,-26,-12,  5;
/M: SY='R';  M=-14,  7,-26,  5,  0,-21,-16, -2,-21, 16,-19, -7,  9,-18,  3, 27, -6, -8,-16,-27,-12,  0;
/M: SY='D';  M= -7, 25,-23, 28,  6,-22, -9, -4,-26,  0,-24,-20, 18,-13, -3, -6,  5,  0,-21,-32,-15,  2;
/M: SY='V';  M= -6,-28,-19,-30,-24,  5,-31,-25, 25,-19, 18, 12,-25,-26,-23,-18,-17, -6, 28,-22, -3,-24;
/M: SY='E';  M= -2, -2,-26,  2, 16,-27, -7,  2,-25,  0,-24,-15, -1,  6,  8, -5,  5, -5,-23,-29,-17, 11;
/M: SY='A';  M=  9, -6,-22, -9,  8,-17,-17,-12, -5, -6, -5, -6, -5,-12, -2, -8, -3, -6, -6,-24,-14,  2;
/M: SY='D';  M=-10, 23,-29, 28,  0,-33, 26, -7,-37, -9,-30,-24, 17,-16, -9,-13,  1,-13,-30,-31,-24, -4;
/M: SY='E';  M= -7, 12,-21, 14, 23,-23,-18, -9,-23,  0,-17,-17,  3, -6,  4, -6,  9, 17,-17,-32,-16, 13;
/M: SY='P';  M= -6,-10,-29,-10, -8,-22, -6,-17,-13,-12,-20,-12, -6, 24, -8,-16, -2, -1,-16,-28,-21,-10;
/M: SY='I';  M= -5,-28,-23,-35,-25,  8,-32,-25, 30,-27, 24, 15,-23,-24,-22,-25,-19, -8, 20,-18,  0,-25;
/M: SY='M';  M= -8,-10,-20,-11, -9, -9,-20, -9, -4, -1, -4,  4, -9,-20, -6,  4, -5,  0,  1,-22, -2, -9;
/M: SY='W';  M=-10,-17,-34,-16, -8, -1,-12,-15,-17,-12,-14, -9,-18,-12,-11,-15,-18,-16,-20, 35,  7, -8;
/M: SY='D';  M= -7, 21,-25, 28, 12,-31,  7, -5,-32, -5,-29,-24, 15,-11, -1,-10, 10, -4,-25,-35,-22,  5;
/M: SY='D';  M= -8, 17,-23, 23,  3,-23,-13, -6,-19, -4,-13, -8,  4,-14, -2, -6,  0, -1,-14,-32,-14,  0;
/M: SY='I';  M=-10,-29,-24,-35,-28, 14,-35,-23, 32,-26, 17, 13,-23,-25,-24,-24,-19, -8, 25,-14,  9,-28;
/M:         M= -4, -7,-25, -8,  0,-16,-16,-16, -5, -3, -3, -3, -8,-15, -5, -8, -8, -6, -5,-24,-11, -4;
/M: SY='N';  M= -9,  8,-23,  0, -2,-21, -5,  0,-15, -2,-16, -8, 18,-19,  9,  3, -1, -6,-18,-29,-15,  3;
/M: SY='K';  M= -8,  4,-28,  6, 11,-23,-18,  8,-25, 17,-22,-10,  0,-12, 10,  8, -6,-10,-21,-19,  0, 10;
/I:         E1=0; IE=-105; DE=-105;

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Numerical results [info]

Numerical results for UniProtKB/Swiss-Prot release 2025_04 which contains 573'661 sequence entries.

Total number of hits	31 in 30 different sequences
Number of true positive hits	31 in 30 different sequences
Number of 'unknown' hits	0
Number of false positive hits	0
Number of false negative sequences	1
Number of 'partial' sequences	0

Precision (true positives / (true positives + false positives))	100.00 %
Recall (true positives / (true positives + false negatives))	96.88 %

Comments [info]

Taxonomic range [info]	Archaea, Eukaryotes, Prokaryotes (Bacteria)
Maximum number of repetitions [info]	2
Matrix type [info]	protein_domain
Scaling database [info]	reversed
Author [info]	CJA_Sigrist
Feature key [info]	DOMAIN
Feature description [info]	AFP-like
Version [info]	5

Cross-references [info]

UniProtKB/Swiss-Prot
True positive sequences

30 sequences

ANP11_ZOAAM (P19613), ANP12_ZOAAM (P19614), ANP1C_ZOAAM (P07457),

ANP1_ANALU  (P12416), ANP1_LYCDA  (P35751), ANP1_LYCPO  (P24028), 
ANP1_PACBR  (P12100), ANP1_ZOAAM  (P19608), ANP2_ANALU  (P12417), 
ANP2_LYCDA  (P12102), ANP2_PACBR  (P12101), ANP3_LYCDA  (P35753), 
ANP3_ZOAAM  (P19606), ANP4_ZOAAM  (P19609), ANP5_ZOAAM  (P19607), 
ANP7_ZOAAM  (P19611), ANP9_ZOAAM  (P19612), ANPD_ZOAAM  (P19604), 
ANPE_ZOAAM  (P19605), NEUAS_CAMJ8 (P0DY06), NEUBH_CAMJE (Q0P8T1), 
NEUBH_LEGPH (Q5ZXH9), PSEI_CAMJE  (Q0P8U0), PSEI_CAMJJ  (Q939J8), 
PSEI_HELPY  (O24980), SIAS_DROME  (Q9VG74), SIAS_HUMAN  (Q9NR45), 
SIAS_MOUSE  (Q99J77), SPSE_BACSU  (P39625), Y1065_METJA (Q58465)

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Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
[Clustal format, color, condensed view] [Clustal format, color]
[Clustal format, plain text] [Fasta format]
Retrieve the sequence logo from the alignment

UniProtKB/Swiss-Prot
False negative sequences

1 sequence

SUYA_CHRSD  (Q1QWP1)

PDB
[Detailed view]

44 PDB

pdb_00001ame; pdb_00001b7i; pdb_00001b7j; pdb_00001b7k; pdb_00001c89; pdb_00001c8a; pdb_00001ekl; pdb_00001gzi; pdb_00001hg7; pdb_00001jab; pdb_00001kde; pdb_00001kdf; pdb_00001msi; pdb_00001msj; pdb_00001ops; pdb_00001ucs; pdb_00001vli; pdb_00001wvo; pdb_00002ame; pdb_00002jia; pdb_00002msi; pdb_00002msj; pdb_00002spg; pdb_00003ame; pdb_00003msi; pdb_00003nla; pdb_00003qf6; pdb_00003rdn; pdb_00004ame; pdb_00004msi; pdb_00004ny6; pdb_00005c7r; pdb_00005msi; pdb_00006ame; pdb_00006msi; pdb_00007ame; pdb_00007msi; pdb_00007q3v; pdb_00008ame; pdb_00008h2c; pdb_00008msi; pdb_00009ame; pdb_00009cbe; pdb_00009msi
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