|PROSITE documentation PDOC00023 [for PROSITE entry PS00024]|
Hemopexin is a serum glycoprotein that binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Structurally hemopexin consists of two similar halves of approximately two hundred amino acid residues connected by a histidine-rich hinge region. Each half is itself formed by the repetition of a basic unit of some 35 to 45 residues. Hemopexin-like domains have been found [1,2] in two other types of proteins:
It is suggested that the hemopexin domain facilitates binding to a variety of molecules and proteins.
The hemopexin domain exhibits the shape of an oblate ellipsoidal disk. The polypeptide chain is organized in four β-sheet (blades) I to IV, which are almost symmetrically arranged around a central axis in consecutive order, giving rise to the formation of a four-bladed propeller (see <PDB:1PEX>). Each propeller blade or repeat is made up of four antiparallel β-strands connected in a W-like strand topology, and is strongly twisted [5,6].
We developed both a pattern and a profile. The signature pattern for the hemopexin domain has been derived from the best conserved region which is located at the beginning of the second repeat. The profile covers the entire hemopexin repeat.Last update:
May 2013 / Text revised; profile added.
PROSITE methods (with tools and information) covered by this documentation:
|1||Authors||Hunt L.T. Barker W.C. Chen H.R.|
|Title||A domain structure common to hemopexin, vitronectin, interstitial collagenase, and a collagenase homolog.|
|Source||Protein Seq. Data Anal. 1:21-26(1987).|
|Title||Homology with hemopexin suggests a possible scavenging function for S-protein/vitronectin.|
|Source||FEBS Lett. 199:249-253(1986).|
|3||Authors||Marioli D.J. Zarkadis I.K.|
|Title||The vitronectin gene in rainbow trout: cloning, expression and phylogenetic analysis.|
|Source||Fish Shellfish Immunol. 24:18-25(2008).|
|4||Authors||Piccard H. Van den Steen P.E. Opdenakker G.|
|Title||Hemopexin domains as multifunctional liganding modules in matrix metalloproteinases and other proteins.|
|Source||J. Leukoc. Biol. 81:870-892(2007).|
|5||Authors||Gomis-Rueth F.X. Gohlke U. Betz M. Knaeuper V. Murphy G. Lopez-Otin C. Bode W.|
|Title||The helping hand of collagenase-3 (MMP-13): 2.7 A crystal structure of its C-terminal haemopexin-like domain.|
|Source||J. Mol. Biol. 264:556-566(1996).|
|6||Authors||Gaur V. Qureshi I.A. Singh A. Chanana V. Salunke D.M.|
|Title||Crystal structure and functional insights of hemopexin fold protein from grass pea.|
|Source||Plant Physiol. 152:1842-1850(2010).|