|PROSITE documentation PDOC00065 [for PROSITE entry PS00067]|
3-hydroxyacyl-CoA dehydrogenase (EC 18.104.22.168) (HCDH)  is an enzyme involved in fatty acid metabolism, it catalyzes the reduction of 3-hydroxyacyl-CoA to 3-oxoacyl-CoA. Most eukaryotic cells have 2 fatty-acid β-oxidation systems, one located in mitochondria and the other in peroxisomes. In peroxisomes 3-hydroxyacyl-CoA dehydrogenase forms, with enoyl-CoA hydratase (ECH) and 3,2-trans-enoyl-CoA isomerase (ECI) a multifunctional enzyme where the N-terminal domain bears the hydratase/isomerase activities and the C-terminal domain the dehydrogenase activity. There are two mitochondrial enzymes: one which is monofunctional and the other which is, like its peroxisomal counterpart, multifunctional.
In Escherichia coli (gene fadB) and Pseudomonas fragi (gene faoA) HCDH is part of a multifunctional enzyme which also contains an ECH/ECI domain as well as a 3-hydroxybutyryl-CoA epimerase domain .
The other proteins structurally related to HCDH are:
There are two major region of similarities in the sequences of proteins of the HCDH family, the first one located in the N-terminal, corresponds to the NAD-binding site, the second one is located in the center of the sequence. We have chosen to derive a signature pattern from this central region.Last update:
December 2004 / Pattern and text revised.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Birktoff J.J., Holden H.M., Hamlin R., Xuong N.-H., Banaszak L.J.|
|Source||Proc. Natl. Acad. Sci. U.S.A. 84:8262-8266(1987).|
|2||Authors||Nakahigashi K., Inokuchi H.|
|Title||Nucleotide sequence of the fadA and fadB genes from Escherichia coli.|
|Source||Nucleic Acids Res. 18:4937-4937(1990).|
|3||Authors||Mullany P., Clayton C.L., Pallen M.J., Slone R., al-Saleh A., Tabaqchali S.|
|Title||Genes encoding homologues of three consecutive enzymes in the butyrate/butanol-producing pathway of Clostridium acetobutylicum are clustered on the Clostridium difficile chromosome.|
|Source||FEMS Microbiol. Lett. 124:61-67(1994).|
|4||Authors||Mulders J.W.M., Hendriks W., Blankesteijn W.M., Bloemendal H., de Jong W.W.|
|Title||Lambda-crystallin, a major rabbit lens protein, is related to hydroxyacyl-coenzyme A dehydrogenases.|
|Source||J. Biol. Chem. 263:15462-15466(1988).|