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PROSITE documentation PDOC00152 [for PROSITE entry PS00168]

Tryptophan synthase beta chain pyridoxal-phosphate attachment site





Description

Tryptophan synthase (EC 4.2.1.20) catalyzes the last step in the biosynthesis of tryptophan: the conversion of indoleglycerol phosphate and serine, to tryptophan and glyceraldehyde 3-phosphate [1,2]. It has two functional domains: one for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate and the other for the synthesis of tryptophan from indole and serine. In bacteria and plants [3], each domain is found on a separate subunit (α and β chains), while in fungi the two domains are fused together on a single multifunctional protein.

The β chain of the enzyme requires pyridoxal-phosphate as a cofactor. The pyridoxal-phosphate group is attached to a lysine residue. The region around this lysine residue also contains two histidine residues which are part of the pyridoxal-phosphate binding site. The signature pattern for the tryptophan synthase β chain is derived from that conserved region.

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html --------------------------------------------------------------------------------.

Last update:

December 2004 / Pattern and text revised.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

TRP_SYNTHASE_BETA, PS00168; Tryptophan synthase beta chain pyridoxal-phosphate attachment site  (PATTERN)


References

1AuthorsCrawford I.P.
TitleEvolution of a biosynthetic pathway: the tryptophan paradigm.
SourceAnnu. Rev. Microbiol. 43:567-600(1989).
PubMed ID2679363
DOI10.1146/annurev.mi.43.100189.003031

2AuthorsHyde C.C. Miles E.W.
TitleThe tryptophan synthase multienzyme complex: exploring structure-function relationships with X-ray crystallography and mutagenesis.
SourceBiotechnology (N.Y.) 8:27-32(1990).
PubMed ID1366510

3AuthorsBerlyn M.B. Last R.L. Fink G.R.
TitleA gene encoding the tryptophan synthase beta subunit of Arabidopsis thaliana.
SourceProc. Natl. Acad. Sci. U.S.A. 86:4604-4608(1989).
PubMed ID2734310



PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

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