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	PROSITE documentation PDOC00163 [for PROSITE entry PS00183]

Ubiquitin-conjugating enzymes signature and profile

Ubiquitin-conjugating enzymes (EC 6.3.2.19) (UBC or E2 enzymes) [1,2,3] catalyze the covalent attachment of ubiquitin to target proteins. An activated ubiquitin moiety is transferred from an ubiquitin-activating enzyme (E1) to E2 which later ligates ubiquitin directly to substrate proteins with or without the assistance of 'N-end' recognizing proteins (E3).

In most species there are many forms of UBC (at least 9 in yeast) which are implicated in diverse cellular functions.

A cysteine residue is required for ubiquitin-thiolester formation. There is a single conserved cysteine in UBC's and the region around that residue is conserved in the sequence of known UBC isozymes. We have used that region as a signature pattern. We also developed a profile that spans the complete catalytical domain.

Expert(s) to contact by email:

Jentsch S.

Last update:

April 2006 / Pattern revised.

PROSITE methods (with tools and information) covered by this documentation:

UBIQUITIN_CONJUGAT_1, PS00183; Ubiquitin-conjugating enzymes active site  (PATTERN)

• Consensus pattern:
  [FYWLSP]-H-[PC]-[NHL]-[LIV]-x(3,4)-G-x-[LIVP]-C-[LIV]-x(1,2)-[LIVR]
  C is the active site residue
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 329
  • detected by PS00183: 240 (true positives)
  • undetected by PS00183: 89 (89 false negatives and 0 'partial')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS00183:
  NONE.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00183
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00183
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS00183
• View ligand binding statistics of PS00183
• Matching PDB structures: 1A3S 1AYZ 1C4Z 1FBV ... [ALL]

UBIQUITIN_CONJUGAT_2, PS50127; Ubiquitin-conjugating enzymes family profile  (MATRIX)

• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 375
  • detected by PS50127: 366 (true positives)
  • undetected by PS50127: 9 (9 false negatives and 0 'partial')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS50127:
  NONE.
• Domain architecture view of Swiss-Prot proteins matching PS50127
  
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS50127
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS50127
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS50127
• View ligand binding statistics of PS50127
• Matching PDB structures: 1A3S 1AYZ 1C4Z 1FBV ... [ALL]

1	Authors	Jentsch S., Seufert W., Sommer T., Reins H.-A.
	Title	Ubiquitin-conjugating enzymes: novel regulators of eukaryotic cells.
	Source	Trends Biochem. Sci. 15:195-198(1990).
	PubMed ID	2193438

2	Authors	Jentsch S., Seufert W., Hauser H.-P.
	Title	Genetic analysis of the ubiquitin system.
	Source	Biochim. Biophys. Acta 1089:127-139(1991).
	PubMed ID	1647207

3	Authors	Hershko A.
	Title	The ubiquitin pathway for protein degradation.
	Source	Trends Biochem. Sci. 16:265-268(1991).
	PubMed ID	1656558

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