Home  |  Contact
PROSITE documentation PDOC00180 [for PROSITE entry PS00203]

Vertebrate metallothioneins signature


Metallothioneins (MT) [1,2,3] are small proteins which bind heavy metals such as zinc, copper, cadmium, nickel, etc., through clusters of thiolate bonds. MT's occur throughout the animal kingdom and are also found in higher plants, fungi and some prokaryotes. On the basis of structural relationships MT's have been subdivided into three classes. Class I includes mammalian MT's as well as MT's from crustacean and molluscs, but with clearly related primary structure. Class II groups together MT's from various species such as sea urchins, fungi, insects and cyanobacteria which display none or only very distant correspondence to class I MT's. Class III MT's are atypical polypeptides containing γ-glutamylcysteinyl units.

Vertebrate class I MT's are proteins of 60 to 68 amino acid residues, 20 of these residues are cysteines that bind to 7 bivalent metal ions. As a signature pattern we chose a region that spans 19 residues and which contains seven of the metal-binding cysteines, this region is located in the N-terminal section of class-I MT's.


This signature pattern is not meant to detect invertebrate class-I MT's whose sequence is highly divergent from that of vertebrate's.

Expert(s) to contact by email:

Binz P.-A.

Last update:

May 2004 / Text revised.

Technical section

PROSITE method (with tools and information) covered by this documentation:

METALLOTHIONEIN_VRT, PS00203; Vertebrate metallothioneins signature  (PATTERN)


1AuthorsHamer D.H.
SourceAnnu. Rev. Biochem. 55:913-951(1986).
PubMed ID3527054

2AuthorsKagi J.H.R. Schaffer A.
TitleBiochemistry of metallothionein.
SourceBiochemistry 27:8509-8515(1988).
PubMed ID3064814

3AuthorsBinz P.-A.
SourceThesis, 1996, University of Zurich.

PROSITE is copyright. It is produced by the SIB Swiss Institute Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified. Usage by and for commercial entities requires a license agreement. For information about the licensing scheme send an email to
Prosite License or see: prosite_license.html.


View entry in original PROSITE document format
View entry in raw text format (no links)