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PROSITE documentation PDOC00186 [for PROSITE entry PS00212] |
The following serum transport proteins are known to be evolutionary related [1,2,3]:
Structurally, these proteins consist of two to seven homologous domains of about 190 amino acids. Each domain, consisting of 10 α-helices, is formed by two smaller subdomains and contains five or six internal disulfide bonds as shown in the following schematic representation [4].
+---+ +----+ +-----+ | | | | | | xxCxxxxxxxxxxxxxxxxCCxxCxxxxCxxxxxCCxxxCxxxxxxxxxCxxxxxxxxxxxxxxCCxxxxCxxxx | | | | | ***|******** +-----------------+ +------+ +---------------+
'C': conserved cysteine involved in a disulfide bond. '*': position of the pattern.
The signature pattern we derived is based on three conserved cysteines at the end of the domain. We built it in such a way that it can detect all 3 repeats in albumin and human afamin, the first two in AFP and the first one in VDB and rat afamin. We also developed a profile, which covers the entire albumin domain.
Last update:March 2009 / Text revised; profile added.
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PROSITE methods (with tools and information) covered by this documentation:
1 | Authors | Haefliger D.N. Moskaitis J.E. Schoenberg D.R. Wahli W. |
Title | Amphibian albumins as members of the albumin, alpha-fetoprotein, vitamin D-binding protein multigene family. | |
Source | J. Mol. Evol. 29:344-354(1989). | |
PubMed ID | 2481749 |
2 | Authors | Schoentgen F. Metz-Boutigue M.-H. Jolles J. Constans J. Jolles P. |
Title | Complete amino acid sequence of human vitamin D-binding protein (group-specific component): evidence of a three-fold internal homology as in serum albumin and alpha-fetoprotein. | |
Source | Biochim. Biophys. Acta 871:189-198(1986). | |
PubMed ID | 2423133 |
3 | Authors | Lichenstein H.S. Lyons D.E. Wurfel M.M. Johnson D.A. McGinley M.D. Leidli J.C. Trollinger D.B. Mayer J.P. Wright S.D. Zukowski M.M. |
Title | Afamin is a new member of the albumin, alpha-fetoprotein, and vitamin D-binding protein gene family. | |
Source | J. Biol. Chem. 269:18149-18154(1994). | |
PubMed ID | 7517938 |
4 | Authors | He X.M. Carter D.C. |
Title | Atomic structure and chemistry of human serum albumin. | |
Source | Nature 358:209-215(1992). | |
PubMed ID | 1630489 | |
DOI | 10.1038/358209a0 |
5 | Authors | Verboven C. Rabijns A. De Maeyer M. Van Baelen H. Bouillon R. De Ranter C. |
Title | A structural basis for the unique binding features of the human vitamin D-binding protein. | |
Source | Nat. Struct. Biol. 9:131-136(2002). | |
PubMed ID | 11799400 | |
DOI | 10.1038/nsb754 |