The proteasome (or macropain) (EC 126.96.36.199) [1,2,3,4,5,6] is a multicatalytic
proteinase complex that seems to be involved in an ATP/ubiquitin-dependent
nonlysosomal proteolytic pathway. The core of this 2.5 MDa enzyme complex is
formed by the 20S proteasome, a barrel-shaped protease of about 700 KDa that
associates with one or two 19S regulatory complexes. The 20S proteasome is
ubiquitous in archaea and in eukaryotes. In bacteria, the 20S has been found
only in actinomycetes.
The main difference between prokaryotic and eukaryotic proteasomes is one of
complexity. Prokaryotic 20S proteasomes contain mostly only to different but
related subunits, α and β (see <PDOC00668>), while eukaryotic
proteasomes contain seven paralogous α-type and seven paralogous β-type
subunits. Subunits that belong to the α-type group are proteins of from
210 to 290 amino acids that share a number of conserved sequence regions.
Some subunits that are known to belong to this family are listed below:
Vertebrate subunits C2 (nu), C3, C8, C9, iota and zeta.
Drosophila PROS-25, PROS-28.1, PROS-29 and PROS-35.
The core of the α subunit is a sandwich of two five-stranded antiparallel
β-sheets. The β sandwich is flanked by the α helices H3, H4, and H5
on top and by H1 and H2 at the bottom (see <PDB:1PMA>) .
As a signature pattern for proteasome A-type subunits we selected the best
conserved region, which is located in the N-terminal part of these proteins.
We also have developed a profile which covers the whole conserved region.
These proteins belong to family T1 in the classification of peptidases
July 2013 / Text and profile revised.
PROSITE methods (with tools and information) covered by this documentation:
PROSITE is copyright. It is produced by the SIB Swiss Institute
Bioinformatics. There are no restrictions on its use by non-profit
institutions as long as its content is in no way modified. Usage by and
for commercial entities requires a license agreement. For information
about the licensing scheme send an email to
or see: prosite_license.html.