|PROSITE documentation PDOC00407 [for PROSITE entry PS00444]|
A variety of isoprenoid compounds are synthesized by various organisms. For example in eukaryotes the isoprenoid biosynthetic pathway is responsible for the synthesis of a variety of end products including cholesterol, dolichol, ubiquinone or coenzyme Q. In bacteria this pathway leads to the synthesis of isopentenyl tRNA, isoprenoid quinones, and sugar carrier lipids. Among the enzymes that participate in that pathway, are a number of polyprenyl synthase enzymes which catalyze a 1'4-condensation between 5 carbon isoprene units.
Currently the sequence of some of these enzymes is known:
It has been shown [1,2,3,4,5] that all the above enzymes share some regions of sequence similarity. Two of these regions are rich in aspartic-acid residues and could be involved in the catalytic mechanism and/or the binding of the substrates. We have developed signature patterns for both regions.
Possible additional members of this family of proteins are:
We believe  that both proteins are most probably also enzymes involved in isoprenoid metabolism.Last update:
November 1997 / Patterns and text revised.
PROSITE methods (with tools and information) covered by this documentation:
|1||Authors||Ashby M.N. Edwards P.A.|
|Title||Elucidation of the deficiency in two yeast coenzyme Q mutants. Characterization of the structural gene encoding hexaprenyl pyrophosphate synthetase.|
|Source||J. Biol. Chem. 265:13157-13164(1990).|
|2||Authors||Fujisaki S. Hara H. Nishimura Y. Horiuchi K. Nishino T.|
|Title||Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli.|
|Source||J. Biochem. 108:995-1000(1990).|
|3||Authors||Carattoli A. Romano N. Ballario P. Morelli G. Macino G.|
|Title||The Neurospora crassa carotenoid biosynthetic gene (albino 3) reveals highly conserved regions among prenyltransferases.|
|Source||J. Biol. Chem. 266:5854-5859(1991).|
|4||Authors||Kuntz M. Romer S. Suire C. Hugueney P. Weil J.H. Schantz R. Camara B.|
|Title||Identification of a cDNA for the plastid-located geranylgeranyl pyrophosphate synthase from Capsicum annuum: correlative increase in enzyme activity and transcript level during fruit ripening.|
|Source||Plant J. 2:25-34(1992).|
|5||Authors||Math S.K. Hearst J.E. Poulter C.D.|
|Title||The crtE gene in Erwinia herbicola encodes geranylgeranyl diphosphate synthase.|
|Source||Proc. Natl. Acad. Sci. U.S.A. 89:6761-6764(1992).|
|Source||Unpublished observations (1993).|