Home  |  Contact
PROSITE documentation PDOC00412 [for PROSITE entry PS00447]

DNA polymerase family A signature





Description

Replicative DNA polymerases (EC 2.7.7.7) are the key enzymes catalyzing the accurate replication of DNA. They require either a small RNA molecule or a protein as a primer for the de novo synthesis of a DNA chain. On the basis of sequence similarities a number of DNA polymerases have been grouped together [1,2,3] under the designation of DNA polymerase family A. The polymerases that belong to this family are listed below.

  • Escherichia coli and various other bacterial polymerase I (gene polA).
  • Thermus aquaticus Taq polymerase.
  • Bacteriophage sp01 polymerase.
  • Bacteriophage sp02 polymerase.
  • Bacteriophage T5 polymerase.
  • Bacteriophage T7 polymerase.
  • Mycobacteriophage L5 polymerase.
  • Yeast mitochondrial polymerase γ (gene MIP1).

Five regions of similarity are found in all the above polymerases. One of these conserved regions, known as 'motif B' [1], is located in a domain which, in Escherichia coli polA, has been shown to bind deoxynucleotide triphosphate substrates; it contains a conserved tyrosine which has been shown, by photo-affinity labelling, to be in the active site; a conserved lysine, also part of this motif, can be chemically labelled, using pyridoxal phosphate. We use this conserved region as a signature for this family of DNA polymerases.

Last update:

November 1995 / Pattern and text revised.

-------------------------------------------------------------------------------

Technical section

PROSITE method (with tools and information) covered by this documentation:

DNA_POLYMERASE_A, PS00447; DNA polymerase family A signature  (PATTERN)


References

1AuthorsDelarue M. Poch O. Tordo N. Moras D. Argos P.
TitleAn attempt to unify the structure of polymerases.
SourceProtein Eng. 3:461-467(1990).
PubMed ID2196557

2AuthorsIto J. Braithwaite D.K.
TitleCompilation and alignment of DNA polymerase sequences.
SourceNucleic Acids Res. 19:4045-4057(1991).
PubMed ID1870963

3AuthorsBraithwaite D.K. Ito J.
TitleCompilation, alignment, and phylogenetic relationships of DNA polymerases.
SourceNucleic Acids Res. 21:787-802(1993).
PubMed ID8451181



PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

Miscellaneous

View entry in original PROSITE document format
View entry in raw text format (no links)